Thermo Scientific™ ABsolute™ qPCR Mix, SYBR Green, ROX
Optimized for SYBR Green chemistry and contains all the components necessary to perform quantitative PCR, with the exception of template and primers.
Manufacturer: Thermo Scientific™ AB1162B
The 2X qPCR SYBR Green Mix contains optimal levels of active SYBR Green I dye and Thermo-Start DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.
ABsolute qPCR SYBR Green Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, Fluorescein or no reference dye. A master mix optimized for capillary instruments is also available.
- High Sensitivity & Specificity - The master mixes contain the patented hot start enzyme Thermo-Start DNA Polymerase
- Optimized buffer containing SYBR Green
- Reproducibility - All master mixes undergo extensive QC to ensure batch to batch consistency
- Broad dynamic range
|heated to 95°C for 15 minutes|
|ABsolute qPCR Buffer (Thermo-Start DNA polymerase, dNTPs, MgCl2, SYBR Green I, ROX™ passive reference dye (only in ROX mixes), Fluorescein (only in Fluorescein mix)); Separate MgCl2; Separate ROX vial (only in kits indicated)|
|-20°C Avoid freeze/thaw cycles|
|7000 System, 7300 System, 7700 System, 7900HT System, StepOne™ System, StepOnePlus™ System|
|ABsolute QPCR SYBR Green ROX (500nM) Mix|
|800 x 50µL rxns|
|Real time PCR (qPCR), gene expression analysis, siRNA validation, genotyping|
|Detection Method: SYBR; PCR Method: qPCR|