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X-Gal, 5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside, Fisher BioReagents™

For blue/white colony screening, immunoblotting and immunocytochemical assays

$43.80 - $160.31

Chemical Identifiers

Chemical Name or Material 5-Bromo-4-chloro-3-indolyl- β-D-galactopyranoside
CAS 7240-90-6
Molecular Formula C14H15BrClNO6
Formula Weight 408.63
Synonym X-Gal
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Catalog Number Mfr. No. Packaging Quantity Price Quantity    


fisher scientific company
Glass Bottle 1g Each for $160.31
Case of 6 Each for $961.86


fisher scientific company
Glass Bottle 100mg Each for $43.80
Case of 6 Each for $262.80
Description & Specifications


Formula Weight 408.63
Melting Point 230°C
Physical Form Powder/Solid
Quantity 1g
Molecular Formula C14H15BrClNO6
Alpha Vector Bromo-4-chloro-3-indolyl-[beta]-D-galactopyranoside (X-Gal)
Color White
Merck Index 15, 10272
Packaging Glass Bottle
CAS 7240-90-6
Specific Rotation -56 to -64° (+ or -)
ChemAlert Storage Symbol Gray
TLC Single spot
Molar Absorption Coefficient 4000 /g-cm. min.
Identification Pass Test
Synonym X-Gal
Recommended Storage -20°C
Chemical Name or Material 5-Bromo-4-chloro-3-indolyl- β-D-galactopyranoside

  • Chromogenic substrate used for blue-white selection of recombinant bacterial colonies with the lac+ genotype
  • Forms blue precipitate when hydrolyzed by β-galactosidase
  • Soluble in DMF and Methanol

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