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Alfa Aesar™ 5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside, 98+%

$45.55 - $298.62

Chemical Identifiers

Chemical Name or Material 5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside
CAS 7240-90-6
Molecular Formula C14H15BrClNO6
Formula Weight 408.64
MDL Number MFCD00005666
Beilstein 1402009
Synonym X-gal
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Products
Catalog Number Mfr. No. Quantity Price Quantity    

AAB21034MA

  SDS
alfa aesar™
B21034MA
10mg Each for $45.55

AAB21034MD

  SDS
alfa aesar™
B21034MD
250mg Each for $298.62

AAB21034MF

  SDS
alfa aesar™
B21034MF
50mg Each for $92.66
Description & Specifications

Specifications

Formula Weight 408.64
Chemical Name or Material 5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside
Assay ≥98%
Molecular Formula C14H15BrClNO6
MDL Number MFCD00005666
CAS 7240-90-6
Melting Point ≈236°C (dec.)
Beilstein 1402009
Synonym X-gal
Quantity 10mg
Optical Rotation −62° (c=1 in 50% DMF)
Recommended Storage 2° to 8°C

Applications
It is a substrate for beta-galactosidase, which cleaves the glycosidic bond to give 5-bromo-4-chloro-3-hydroxy-1H-indole, which immediately dimerises to give an intensely blue product. It is also used to detect activity of this enzyme in histochemistry and bacteriology. It is employed as a colorless, endogenous or exogenous pigment precursors that may be transformed by biological mechanisms into colored compounds. They are used in biochemical assays and in diagnosis as indicators, particularly in the form of enzyme substrates. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.

Solubility
Soluble in 100 mM in DMSO.

Notes
Moisture & Light sensitive. Store away from oxidizing agents, heat, water/moisture and light. Store under inert gas. Keep the container tightly closed and place it in a cool, dry and well ventilated condition.

Safety and Handling

2° to 8°C