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Alfa Aesar™ 3,3'-Diaminobenzidine tetrahydrochloride hydrate

Widely used peroxidase substrate for immunoblotting and immunohistochemical staining techniques

$166.55 - $289.92

Chemical Identifiers

CAS 868272-85-9
Molecular Formula C12H16N4O
Molecular Weight (g/mol) 232.287
MDL Number MFCD08273058
InChI Key WWIHRTPUMJFLTN-UHFFFAOYSA-N
Synonym 3,3'-diaminobenzidine tetrahydrochloride hydrate, 1,1'-biphenyl-3,3',4,4'-tetraamine tetrahydrochloride hydrate, acmc-209qbm, 3,3'-diaminobenzidine hydrate tetrahydrochloride, 3,3'-diaminobenzidine tetrahydrochloride hydrate, biphenyl-3,3',4,4'-tetramine tetrahydrochloride hydrate, 3,3'-diaminobenzidine tetrahydrochloride hydrate, isopac r, 1,1'-biphenyl-3,3',4,4'-tetraamine tetrahydrochloride xhydrate, 3,3 inverted exclamation marka-diaminobenzidine tetrahydrochloride hydrate, 3,3'-diaminobenzidine tetrahydrochloride hydrate, for spectrophotometric det. of se
PubChem CID 23191111
IUPAC Name 4-(3,4-diaminophenyl)benzene-1,2-diamine;hydrate
SMILES C1=CC(=C(C=C1C2=CC(=C(C=C2)N)N)N)N.O
View More Specs

Products 2
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
AAJ6221606 View Documents Alfa Aesar™
J6221606
5g Each for $166.55
 
AAJ6221609 View Documents Alfa Aesar™
J6221609
10g Each for $289.92
 
Description

Description

Applications
3,3′-diaminobenzidine-tetrahydrochloride-dihydrate (DAB) was used as a chromogen, for detecting the antigen-antibody complex. It is one of the most potent marker being used in immunochemistry, mostly at the electron and light microscope levels. It is used as a histological stain for peroxidase activity. Reaction between 3,3′-Diaminobenzidine and peroxidase forms a visible insoluble product. This approach has been used to assay activity of glutamate decarboxylase in rat cerebellar sections and to detect horseradish peroxidase presence on cultured cell surfaces.

Solubility
Soluble at 5%(w/v) in water

Notes
Store at room temperature. The powder has a shelf-life of 3 years. Store in cool place. Keep container tightly closed in a dry and well-ventilated place. Light sensitive. hygroscopic Store with desiccant. Handle under nitrogen, protect from moisture. Store under nitrogen
Specifications

Chemical Identifiers

868272-85-9
232.287
WWIHRTPUMJFLTN-UHFFFAOYSA-N
23191111
C1=CC(=C(C=C1C2=CC(=C(C=C2)N)N)N)N.O
C12H16N4O
MFCD08273058
3,3'-diaminobenzidine tetrahydrochloride hydrate, 1,1'-biphenyl-3,3',4,4'-tetraamine tetrahydrochloride hydrate, acmc-209qbm, 3,3'-diaminobenzidine hydrate tetrahydrochloride, 3,3'-diaminobenzidine tetrahydrochloride hydrate, biphenyl-3,3',4,4'-tetramine tetrahydrochloride hydrate, 3,3'-diaminobenzidine tetrahydrochloride hydrate, isopac r, 1,1'-biphenyl-3,3',4,4'-tetraamine tetrahydrochloride xhydrate, 3,3 inverted exclamation marka-diaminobenzidine tetrahydrochloride hydrate, 3,3'-diaminobenzidine tetrahydrochloride hydrate, for spectrophotometric det. of se
4-(3,4-diaminophenyl)benzene-1,2-diamine;hydrate

Specifications

Gray
868272-85-9
MFCD08273058
Soluble at 5%(w/v) in water
C1=CC(=C(C=C1C2=CC(=C(C=C2)N)N)N)N.O
232.287
360.11 (Anhydrous)
3,3'-Diaminobenzidine tetrahydrochloride hydrate
5g
C12H16N4O
3,3'-diaminobenzidine tetrahydrochloride hydrate, 1,1'-biphenyl-3,3',4,4'-tetraamine tetrahydrochloride hydrate, acmc-209qbm, 3,3'-diaminobenzidine hydrate tetrahydrochloride, 3,3'-diaminobenzidine tetrahydrochloride hydrate, biphenyl-3,3',4,4'-tetramine tetrahydrochloride hydrate, 3,3'-diaminobenzidine tetrahydrochloride hydrate, isopac r, 1,1'-biphenyl-3,3',4,4'-tetraamine tetrahydrochloride xhydrate, 3,3 inverted exclamation marka-diaminobenzidine tetrahydrochloride hydrate, 3,3'-diaminobenzidine tetrahydrochloride hydrate, for spectrophotometric det. of se
WWIHRTPUMJFLTN-UHFFFAOYSA-N
4-(3,4-diaminophenyl)benzene-1,2-diamine;hydrate
23191111
Powder
Safety and Handling

Safety and Handling

GHS H Statement
H351-H315-H319-H335
Suspected of causing cancer.
Causes skin irritation.
Causes serious eye irritation.
May cause respiratory irritation.

GHS P Statement
P261-P280-P281-P305+P351+P338-P405-P501a
Avoid breathing dust/fume/gas/mist/vapors/spray.
Wear protective gloves/protective clothing/eye protection/face protection.
Use personal protective equipment as required.
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Store locked up.
Dispose of contents/container in accordance with local/regional/national/international regulations.

Warning

TSCA : TSCA

Recommended Storage : −30° to −10°C