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3,3',5,5'-Tetramethylbenzidine soln., Ready-to-Use, high sensitivity, Thermo Scientific Chemicals

3,3’,5,5’-Tetramethylbenzidine (TMB, CAS # 54827-17-7) is a blue chromogenic substance used in staining techniques such as immunohistochemistry.

Supplier:  Thermo Scientific Chemicals J61325AU

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Catalog No. AAJ61325AU



Description

Description

TMB is a substrate for horseradish peroxidase. Recommended for ELISA (microwell) procedures,3,3?,5,5?-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3?,5,5?-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

• TMB is an aromatic amine that acts as a hydrogen donor in horseradish peroxidase-catalyzed reactions
• The transfer of hydrogen results in a blue stain associated with the target of interest
• This pH-dependent color change can be confirmed using spectrophotometric methods
• TMB is commonly used in immunohistochemistry; TMB is light-sensitive, and all applications should be performed in the dark

Application

• TMB is suitable for immunohistochemistry and immunocytochemistry applications including ELISA and cell and tissue staining

Specifications

Specifications

3,3',5,5'-Tetramethylbenzidine soln.
54827-17-7
C16H20N2
1000 mL
2808541
Insoluble in water. Soluble in organic solvents such as DMSO,96 % Ethanol,Acetone,Chloroform,Toluene.
CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C
240.35
240.35
Ready-to-Use, high sensitivity
Yellow
MFCD00007748
UN2810
3,3',5,5'-tetramethylbenzidine, 3,3',5,5'-tetramethyl-1,1'-biphenyl-4,4'-diamine, 3,5,3',5'-tetramethylbenzidine, tmb, 3,3',5,5'-tetramethyl benzidine, tmb substrate, bm blue, ccris 4727, tmb plus, tmb, insoluble
UAIUNKRWKOVEES-UHFFFAOYSA-N
4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline
41206
Liquid

Chemical Identifiers

54827-17-7
240.35
UAIUNKRWKOVEES-UHFFFAOYSA-N
41206
CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C
C16H20N2
MFCD00007748
3,3',5,5'-tetramethylbenzidine, 3,3',5,5'-tetramethyl-1,1'-biphenyl-4,4'-diamine, 3,5,3',5'-tetramethylbenzidine, tmb, 3,3',5,5'-tetramethyl benzidine, tmb substrate, bm blue, ccris 4727, tmb plus, tmb, insoluble
4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline
Safety and Handling

Safety and Handling

GHS H Statement
H370-H312-H332
Causes damage to organs.
Harmful in contact with skin.
Harmful if inhaled.

P210-P233-P235-P240-P241-P242-P243-P260-P264b-P270-P271-P280-P301+P312-P303+P361+P353-P304+P340-P307+P311-P311-P312-P330-P363-P370+P378q-P501c

H226-H302+H312-H331-H336-H370

DOTInformation : Transport Hazard Class: 6.1; Packing Group: III; Proper Shipping Name: TOXIC, LIQUIDS, ORGANIC, N.O.S.

EINECSNumber : 259-364-6

TSCA : Yes

Recommended Storage : Keep cold

SDS
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RUO – Research Use Only