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Alfa Aesar™ 3,3',5,5'-Tetramethylbenzidine aq. soln.

Chemical Identifiers

Chemical Name or Material 3,3',5,5'-Tetramethylbenzidine
CAS 54827-17-7
Molecular Formula C16H20N2
Formula Weight 240.35g/mol
MDL Number MFCD00007748
Beilstein 2808541
Synonym TMB soluble reagent, aq. soln.
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Products
Catalog Number Mfr. No. Quantity Price Quantity    

AAJ60808EQE

 
alfa aesar™
J60808-EQE
125mL CALL N/A N/A
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Description & Specifications

Specifications

Quantity 125mL
Color Pale Yellow
Quantity 125mL
Chemical Name or Material 3,3',5,5'-Tetramethylbenzidine
CAS 54827-17-7
Physical Form Liquid
Molecular Formula C16H20N2
Formula Weight 240.35g/mol
MDL Number MFCD00007748
Beilstein 2808541
Synonym TMB soluble reagent, aq. soln.
Recommended Storage 2° to 8°C
TSCA TSCA

Applications
3,3’,5,5’-Tetramethylbenzidine or TMB is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays. 3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood assay of hemoglobin assay of peroxidases.

Solubility
Slightly soluble. <0.1 g/100 mL at 20°C.

Notes
Contains TMB in an aqueous buffer, pH 3.6 ± 0.2, with 0.03% hydrogen peroxide. The TMB product is soluble after conversion

Safety and Handling

2° to 8°C