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Thermo Scientific 1-Step Human High-Yield In Vitro Expression Kits

Express functional proteins with 10 to 100 times greater yield per mL than other mammalian IVT systems with Thermo Scientific™ 1-Step Human High-Yield In Vitro Translation (IVT) Kits.

Manufacturer: thermo scientific  88892

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Catalog No. PI88892

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Description & Specifications


Kit Contents 5X Dialysis Buffer, 23mL
Quantity 2 x 2mL kit
Sufficient For 2 reactions of 2mL each
Formulation Multi-component kit
Description 1-Step Human High-Yield Maxi IVT Kit

The Thermo Scientific™ 1-Step Human High-Yield In Vitro Translation (IVT) Kits with continuous-feed device enables the expression of functional proteins with 10 to 100 times greater yield per mL than other eukaryotic IVT translation systems.

The 1-Step High-Yield IVT System uses modified HeLa cell extracts to take advantage of the robust human transcription and translation machinery and generate functional full-length proteins. In this system, protein expression is performed in a proprietary mini dialysis device that allows a continuous supply of nucleotides, amino acids and energy generating substrates into the reaction while removing inhibitors of proteins synthesis. This continuous-exchange cell-free (CECF) system enables protein expression for up to 24 hours and produces protein yields ranging from 250 to 750μg/mL. The complete kits come with all components required for proper transcription and translation of recombinant protein, including an optimized expression vector.


  • High expression – up to 750μg/mL of expressed protein

  • Reproducibility – low variability between experiments

  • Fast – express high levels of protein with an overnight incubation

  • Functional – obtain more active protein than with bacterial protein expression systems

1-Step Protein Expression:

  • Streamlined reaction – requires only five components

  • Easy-to-use – transcription and translation is performed in one reaction step

  • Adapt for labeling – amenable to incorporation of heavy or unnatural amino acids


Complete kits contain cell lysate, reaction mix, accessory proteins, 5X dialysis buffer, control DNA, cloning vector, mini dialysis devices and centrifuge tubes


30°C incubator or water bath

Recommended for:

Production of recombinant proteins for structural analysis; Generate active proteins for protein interaction studies; Perform rapid expression and characterization of mutant proteins; Small-scale production of virual particles; Incorporation of unnatural amino acids or toxic protein production