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A dual hot-start mechanism for excellent specificity<\/li>
Highly reproducible CTs over a broad dynamic range<\/li>
Inclusion of UDG to help prevent carryover contamination<\/li>
Stability of pre-assembled reactions for up to 72 hours<\/li>
Compatibility with most real-time qPCR instruments<\/li><\/ul>
Exceptional specificity with dual hot-start mechanism:<\/p>
- Specificity is paramount in obtaining high-quality data in SYBR™ Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding<\/li>
- PowerUp™ SYBR™ Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures<\/li>
- In an experiment evaluating 24 different primer sets, PowerUp™ SYBR™ Green Master Mix generated single melt curves 100% of the time, consistent with highly specific amplification<\/li><\/ul>
  Tight reproducibility in CTs over a wide dynamic range:<\/p>
  - PowerUp™ SYBR™ Green Master Mix demonstrates excellent reproducibility over a wide dynamic range for a variety of targets tested<\/li>
  - In addition, this master mix provides efficient amplification over 6 logs of sample input<\/li>
  - Unlike competitor mixes that can exhibit inhibition at high cDNA inputs and low correlation coefficients over a dynamic range experiment, PowerUp™ SYBR™ Green Master Mix efficiency is maintained over the full dynamic range to help deliver maximal confidence in data quality<\/li><\/ul>
    UDG included for carryover contamination control:<\/p>
    - The inclusion of uracil-DNA glycosylase (UDG) and dUTP in the PowerUp™ SYBR™ Green Master Mix enables the degradation of any previously amplified PCR products, helping prevent contamination of subsequent qPCR reactions and possible false positives<\/li><\/ul>
      Stability of pre-assembled reactions:<\/p>
      - The tight control of the Dual-Lock™ Taq DNA polymerase in PowerUp™ SYBR™ Green Master Mix enables reactions to be assembled up to 72 hours prior to cycling without impacting data<\/li>
      - This consistency allows users to have confidence and flexibility in the use of PowerUp™ SYBR™ Green Master Mix across numerous workflow scenarios1<\/li><\/ul>
        Broad instrument compatibility:<\/p>
        PowerUp™ SYBR™ Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems™ real-time PCR instruments2<\/li>
        It is also compatible with the Bio-Rad IQ™5 and CFX96™\/CFX384™ systems, Roche LightCycler™ LC480, and Stratagene™ MX3005P™ systems<\/li>
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        Highly reproducible CTs over a broad dynamic range<\/li>
        Inclusion of UDG to help prevent carryover contamination<\/li>
        Stability of pre-assembled reactions for up to 72 hours<\/li>
        Compatibility with most real-time qPCR instruments<\/li><\/ul>
        Exceptional specificity with dual hot-start mechanism:<\/p>
        Specificity is paramount in obtaining high-quality data in SYBR™ Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding<\/li>
        PowerUp™ SYBR™ Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures<\/li>
        In an experiment evaluating 24 different primer sets, PowerUp™ SYBR™ Green Master Mix generated single melt curves 100% of the time, consistent with highly specific amplification<\/li><\/ul>
        Tight reproducibility in CTs over a wide dynamic range:<\/p>
        PowerUp™ SYBR™ Green Master Mix demonstrates excellent reproducibility over a wide dynamic range for a variety of targets tested<\/li>
        In addition, this master mix provides efficient amplification over 6 logs of sample input<\/li>
        Unlike competitor mixes that can exhibit inhibition at high cDNA inputs and low correlation coefficients over a dynamic range experiment, PowerUp™ SYBR™ Green Master Mix efficiency is maintained over the full dynamic range to help deliver maximal confidence in data quality<\/li><\/ul>
        UDG included for carryover contamination control:<\/p>
        The inclusion of uracil-DNA glycosylase (UDG) and dUTP in the PowerUp™ SYBR™ Green Master Mix enables the degradation of any previously amplified PCR products, helping prevent contamination of subsequent qPCR reactions and possible false positives<\/li><\/ul>
        Stability of pre-assembled reactions:<\/p>
        The tight control of the Dual-Lock™ Taq DNA polymerase in PowerUp™ SYBR™ Green Master Mix enables reactions to be assembled up to 72 hours prior to cycling without impacting data<\/li>
        This consistency allows users to have confidence and flexibility in the use of PowerUp™ SYBR™ Green Master Mix across numerous workflow scenarios1<\/li><\/ul>
        Broad instrument compatibility:<\/p>
        PowerUp™ SYBR™ Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems™ real-time PCR instruments2<\/li>
        It is also compatible with the Bio-Rad IQ™5 and CFX96™\/CFX384™ systems, Roche LightCycler™ LC480, and Stratagene™ MX3005P™ systems<\/li>
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        A dual hot-start mechanism for excellent specificity<\/li>
        Highly reproducible CTs over a broad dynamic range<\/li>
        Inclusion of UDG to help prevent carryover contamination<\/li>
        Stability of pre-assembled reactions for up to 72 hours<\/li>
        Compatibility with most real-time qPCR instruments<\/li><\/ul>
        Exceptional specificity with dual hot-start mechanism:<\/p>
        Specificity is paramount in obtaining high-quality data in SYBR™ Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding<\/li>
        PowerUp™ SYBR™ Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures<\/li>
        In an experiment evaluating 24 different primer sets, PowerUp™ SYBR™ Green Master Mix generated single melt curves 100% of the time, consistent with highly specific amplification<\/li><\/ul>
        Tight reproducibility in CTs over a wide dynamic range:<\/p>
        PowerUp™ SYBR™ Green Master Mix demonstrates excellent reproducibility over a wide dynamic range for a variety of targets tested<\/li>
        In addition, this master mix provides efficient amplification over 6 logs of sample input<\/li>
        Unlike competitor mixes that can exhibit inhibition at high cDNA inputs and low correlation coefficients over a dynamic range experiment, PowerUp™ SYBR™ Green Master Mix efficiency is maintained over the full dynamic range to help deliver maximal confidence in data quality<\/li><\/ul>
        UDG included for carryover contamination control:<\/p>
        The inclusion of uracil-DNA glycosylase (UDG) and dUTP in the PowerUp™ SYBR™ Green Master Mix enables the degradation of any previously amplified PCR products, helping prevent contamination of subsequent qPCR reactions and possible false positives<\/li><\/ul>
        Stability of pre-assembled reactions:<\/p>
        The tight control of the Dual-Lock™ Taq DNA polymerase in PowerUp™ SYBR™ Green Master Mix enables reactions to be assembled up to 72 hours prior to cycling without impacting data<\/li>
        This consistency allows users to have confidence and flexibility in the use of PowerUp™ SYBR™ Green Master Mix across numerous workflow scenarios1<\/li><\/ul>
        Broad instrument compatibility:<\/p>
        PowerUp™ SYBR™ Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems™ real-time PCR instruments2<\/li>
        It is also compatible with the Bio-Rad IQ™5 and CFX96™\/CFX384™ systems, Roche LightCycler™ LC480, and Stratagene™ MX3005P™ systems<\/li>
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        A dual hot-start mechanism for excellent specificity<\/li>
        Highly reproducible CTs over a broad dynamic range<\/li>
        Inclusion of UDG to help prevent carryover contamination<\/li>
        Stability of pre-assembled reactions for up to 72 hours<\/li>
        Compatibility with most real-time qPCR instruments<\/li><\/ul>
        Exceptional specificity with dual hot-start mechanism:<\/p>
        Specificity is paramount in obtaining high-quality data in SYBR™ Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding<\/li>
        PowerUp™ SYBR™ Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures<\/li>
        In an experiment evaluating 24 different primer sets, PowerUp™ SYBR™ Green Master Mix generated single melt curves 100% of the time, consistent with highly specific amplification<\/li><\/ul>
        Tight reproducibility in CTs over a wide dynamic range:<\/p>
        PowerUp™ SYBR™ Green Master Mix demonstrates excellent reproducibility over a wide dynamic range for a variety of targets tested<\/li>
        In addition, this master mix provides efficient amplification over 6 logs of sample input<\/li>
        Unlike competitor mixes that can exhibit inhibition at high cDNA inputs and low correlation coefficients over a dynamic range experiment, PowerUp™ SYBR™ Green Master Mix efficiency is maintained over the full dynamic range to help deliver maximal confidence in data quality<\/li><\/ul>
        UDG included for carryover contamination control:<\/p>
        The inclusion of uracil-DNA glycosylase (UDG) and dUTP in the PowerUp™ SYBR™ Green Master Mix enables the degradation of any previously amplified PCR products, helping prevent contamination of subsequent qPCR reactions and possible false positives<\/li><\/ul>
        Stability of pre-assembled reactions:<\/p>
        The tight control of the Dual-Lock™ Taq DNA polymerase in PowerUp™ SYBR™ Green Master Mix enables reactions to be assembled up to 72 hours prior to cycling without impacting data<\/li>
        This consistency allows users to have confidence and flexibility in the use of PowerUp™ SYBR™ Green Master Mix across numerous workflow scenarios1<\/li><\/ul>
        Broad instrument compatibility:<\/p>
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