Gene Reporter Systems

Promega ENLITEN™ ATP Assay System DFS Item

The ENLITEN ATP Assay System is used to measure ATP levels for the indirect detection of biocontamination.

Promega Dual-Luciferase™ Reporter (DLR™) Assay Systems DFS Item

The Dual-Luciferase Reporter (DLR) Assay System provides an efficient means of performing two reporter assays. In the DLR Assay, the activities of firefly and Renilla luciferases are measured sequentially from a single sample.

Promega ADP-Glo™ Kinase Assay DFS Item

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is converted into light by Ultra-Glo™ Luciferase. The luminescent signal positively correlates with kinase activity.

Promega Luciferase Assay Systems DFS Item

An extremely sensitive reagent for rapid quantitation of firefly luciferase. Can be used for assays in single-tube luminometers or in multiwell-plate luminometers with auto-injectors.

Promega NAD/NADH-Glo™ Assay DFS Item

The NAD/NADH-Glo™ Assay is a bioluminescent, homogeneous single-reagent-addition assay for detecting total oxidized and reduced nicotinamide adenine dinucleotides and determining their ratio in biological samples or in defined enzyme reactions.

Invitrogen™ S1 Nuclease DFS Item

Single-strand-specific endonuclease hydrolyzes single-stranded RNA or DNA into 5' mononucleotides

Promega Bright-Glo™ Luciferase Assay System DFS Item

Bright-Glo™ Luciferase Assay System is designed for continuous-process systems. The robust, homogeneous assay chemistry gives high assay sensitivity and has a 30-minute signal half life.

Promega Dual-Glo™ Luciferase Assay System DFS Item

A homogeneous reagent system that enables fast, simple quantitation of both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed.

Promega ENLITEN™ Luciferase/Luciferin Reagent DFS Item

The ENLITEN rLuciferase/Luciferin Reagent is intended for the rapid and quantitative detection of ATP in liquid samples.

Promega Luciferase Reporter Assays to Study Nuclear Receptors: pBIND-ERα Vector DFS Item

NR ligand binding domain fused to yeast GAL4 TF DBD in the FN26A (BIND) Flexi Vector or use pBIND-Er∝ and pBIND-GR Vectors. Fusion protein NR activates luc2P reporter controlled by 9X GAL4 UAS in pGL4.35. Use pGL4.36 MMTV LTR for androgen or GC responses.

Invitrogen™ X-Gal (5-Bromo-4-Chloro-3-Indolyl β-D-Galactopyranoside) DFS Item

Widely used chromogenic substrate for β-galactosidase

Promega Pgp-Glo™ Assay System DFS Item

The Pgp-Glo™ Assay Systems provide the necessary reagents for performing luminescent P-glycoprotein (Pgp) ATPase assays.

Promega ONE-Glo™ Luciferase Assay System DFS Item

The ONE-Glo™ Luciferase Assay System provides a sensitive, robust, homogeneous assay for detection of firefly luciferase reporter gene expression in mammalian cells. Ideal for high- and ultrahigh-throughput applications.

Promega Steady-Glo™ Luciferase Assay System DFS Item

Steady-Glo™ Luciferase Assay System is a homogeneous assay with signal half-life of over 5 hours in commonly used cell culture media. Ideal for batch processing.

Promega Coelenterazines DFS Item

We offer Coelenterazine (native) and Coelenterazine-h. Coelenterazine (native) is the luminescent substrate for Renilla luciferase and apoaequorin.

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with One Shot™ TOP10 chemically competent E. coli cells DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (“TOPO Cloning”) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for subcloning

Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, without competent cells DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (“TOPO Cloning”) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing

Invitrogen™ BLOCK-iT™ Lentiviral RNAi Expression System DFS Item

Provides a pLenti6/BLOCK-iT-DEST expression vector to efficiently introduce and stably express short hairpin RNA (shRNA) in vivo from a lentiviral vector

Invitrogen™ Biotin-14-dATP DFS Item

A patented dATP analog with biotin attached at the 6-position of the purine base by a 14-atom linker

Promega Luciferase Assay System Components DFS Item

For use with Promega Luciferase Assay Systems (see cat. no. PRE1500)

Invitrogen™ Biotin-14-dCTP DFS Item

Used for DNA biotinylation, an excellent, and sometimes superior alternative to conventional radioisotopic labeling

Promega β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer DFS Item

The Beta-Galactosidase Enzyme Assay System with Reporter Lysis Buffer is used to assay beta-galactosidase activity in cell lysates.

Promega Dual-Luciferase™ Reporter (DLR™) Assay System Lysis 5X Buffer DFS Item

For use with Promega Dual-Luciferase Reporter (DLR) Assay Systems (Cat. No. PR-E1910)

Invitrogen™ ArrayControl™ RNA Spikes DFS Item

Add to the RNA sample for use as a template for preparing labeled cDNA