Réactifs, membranes et fournitures pour Western blot

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity.  Cytiva Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

MilliporeSigma™ Immobilon™-P PVDF Transfer Membranes

Optimized for binding proteins transferred from a variety of gel matrices. Immobilon™-P PVDF Transfer Membranes have superior handling, staining, and resistance than nitrocellulose. For use with Western blots and protein sequencing. Available in sheets or rolls.

MilliporeSigma™ Luminata™ Western HRP Chemiluminescence Substrates

Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns

Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

MilliporeSigma™ Immobilon™-FL PVDF Transfer Membranes

Designed specifically for Western blots and protein sequencing. Immobilon™-FL PVDF Transfer Membranes are plain surfaced with 0.45μm pores and have lower background fluorescence than other membranes. Available in 10cm and 20cm squares and a 3.75m roll.

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls

Use for the best results with fluorescence Western blotting detection applications. Cytiva Life Sciences™ Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ ECL™ Western Blotting Detection Reagents

Use for all routine Western blotting. Cytiva Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world’s first commercially available chemiluminescent detection reagents and still one of the most widely used.

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ ECL Select™ Western Blotting Detection Reagent

Use as a high sensitivity reagent for chemiluminescent Western blotting detection. Cytiva Life Sciences™ Amersham™ ECL Select™ Western Blotting Detection Reagent  provides highly intense signal output, for detection of medium to very low protein levels.

MilliporeSigma™ Immobilon-FL PVDF Membrane

Immobilon-FL PVDF Membrane do not crack or curl, and can be cut without fracturing

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls

Use this versatile nitrocellulose membrane for excellent binding affinity for small proteins and peptides, as well as nucleic acids. Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

Thermo Scientific™ Pierce™ DAB Substrate Kit

Chromogenic horseradish peroxidase (HRP) substrate for Western blot and tissue staining methods.

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate

Luminol-based enhanced chemiluminescence (ECL) HRP substrate with very stable light output for femtogram-level detection with CCD-based imagers.

Invitrogen™ Incubation Tray, 10 x 14 cm DFS Item

Enables convenient and even staining of E-PAGE™ blots

Invitrogen™ PVDF/Filter Paper Sandwich, 0.2 µm, 8.3 x 7.3 cm DFS Item

Pre-cut and pre-assembled—ready for protein blotting/transfer

Thermo Scientific™ SuperSignal™ West Pico PLUS and SuperSignal™ West Femto Sample

Provides chemiluminescent detection substrates for both routine and high sensitivity western blots

Thermo Scientific™ 1-Step™ Ultra TMB-Blotting Solution

Thermo Scientific™ 1-Step Ultra TMB-Blotting Solution is an enhanced single-component horseradish peroxidase (HRP) substrate for Western blotting and immunohistochemistry.

Thermo Scientific™ PVDF Transfer Membrane, 0.2 µm, 26.5 cm x 3.75 m

Thermo Scientific Pierce PVDF Transfer Membranes are made of high-quality polyvinylidene difluoride and provide high binding capacity for proteins and nucleic acids for Western, Southern, and Northern blotting methods. This PVDF membrane has a 0.2-micron pore size and is ideal for colorimetric and chemiluminescent detection.

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate: PROMO

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Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Thermo Scientific™ Owl™ Blotting Paper

Absorb adequate amounts of buffer for semi-dry electroblotting without the loss of mechanical stability with this high-quality blotting paper.

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ Protran™ Supported NC Nitrocellulose Membranes: Rolls

Use for robust protein blotting. Cytiva Life Sciences™ Amersham™ Protran™ Supported NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

Invitrogen™ iBind™ Flex Western Starter Kit DFS Item

Allows better antigen-antibody interaction and solutions flow between the iBind™ Card and membrane for maximum interaction between protein and antibody

MilliporeSigma™ Immobilon-NC Transfer Membrane

Maximum sensitivity with minimal background, suitable for both chemiluminescent and radioactive detection systems

Thermo Scientific™ SuperSignal™ West Femto and SuperSignal™ West Pico PLUS Sample

Provides chemiluminescent detection substrates for both routine and high sensitivity western blots

Invitrogen™ Invitrolon™ PVDF/Filter Paper Sandwiches, 0.45 µm, 8.3 x 7.3 cm (for mini gels) DFS Item

Pre-cut and pre-assembled and ready for protein blotting/transfer

Invitrogen™ Novex™ Reversible Membrane Protein Stain Kit DFS Item

Used for determining protein transfer efficiency after blotting

Cytiva (Formerly GE Healthcare Life Sciences) Whatman™ Gel Blotting Paper Grade GB003

A thick paper ideal for lysis/denaturation of colony or plaque lifts and Western blots

Fisherbrand™ Polypropylene Microtube Support Racks

Excellent for microbiology work or any work using microtubes

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