Restriction Enzymes

Restriction enzymes cleave DNA into fragments at or near specific recognition sequences known as restriction sites; also known as restriction endonuclease, or restrictase. Products include various restriction enzymes, kits, buffers, etc.

1 – 30 de 33 résultats

OPTIZYME™ NotI, Fisher BioReagents™

Prix et disponibilité
Spécifications

Tampon de stockage	20mM Tris HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol
Format	Liquid
Composants	25 to 50% Water, >50% Glycerin
Type de produit	NotI
Site de coupe	GC.GGCCGC
pH	7.4
Concentration	10 U/μL
Contenu et stockage	Keep container tightly closed
À utiliser avec (application)	>90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Not I

Tampon de stockage	10mM Tris HCl (pH 7.5 at 25°C), 250mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Température de l’incubateur	37°C
Format	Liquid
Composants	25 to 50% Water, >50% Glycerin, 1 to 2.5% Potassium Chloride
Type de produit	HindIII
Site de coupe	A.AGCTT
Concentration	10 U/μL
Contenu et stockage	Keep container tightly closed
À utiliser avec (application)	>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with HaeIII

OPTIZYME™ BamHI, Fisher BioReagents™

Prix et disponibilité
Spécifications

Tampon de stockage	10mM Tris HCl (pH 7.4 at 25°C), 200mM NaCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol
Température de l’incubateur	37°C
Format	Liquid
Composants	25 to 50% Water, >50% Glycerin, 1 to 2.5% Sodium Chloride
Type de produit	BamHI
Site de coupe	G.GATCC
pH	7.4
Concentration	10 U/μL
Contenu et stockage	Keep container tightly closed
À utiliser avec (application)	>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BamHI

OPTIZYME™ EcoRI, Fisher BioReagents™

Prix et disponibilité
Spécifications

Tampon de stockage	10mM Potassium Phosphate (pH 7.4 at 25°C), 300mM NaCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 0.15% Triton™ X-100, 50% Glycerol
Température de l’incubateur	37°C
Format	Liquid
Type de produit	EcoRI
Site de coupe	G.AATTC
pH	7.4
Contenu et stockage	Keep container tightly closed
À utiliser avec (application)	>90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with EcoRI

OPTIZYME™ BglII, Fisher BioReagents™

Prix et disponibilité
Spécifications

Tampon de stockage	10mM Tris HCl (pH 8.2 at 25°C), 200mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol
Température de l’incubateur	37°C
Format	Liquid
Composants	25 to 50% Water, >50% Glycerin, 1 to 2.5% Sodium Chloride
Type de produit	BglII
Site de coupe	A.GATCT
pH	7.4
Concentration	10 U/μL
Contenu et stockage	Keep container tightly closed
À utiliser avec (application)	>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BglII

OPTIZYME™ XbaI, Fisher BioReagents™

Prix et disponibilité
Spécifications

Tampon de stockage	10mM Tris HCl (pH 7.4), 100mM KCl, 7mM 2-mercaptoethano, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Température de l’incubateur	37°C
Format	Liquid
Composants	10X OPTIZYME™ Buffer 4
Type de produit	XbaI
Site de coupe	T.CTAGA
pH	7.4
À utiliser avec (application)	Indicates ligation efficiency of DNA fragments generated by digestion with the Restriction Enzyme

OPTIZYME™ SmaI, Fisher BioReagents™

Prix et disponibilité
Spécifications

Tampon de stockage	10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 50% Glycerol
Température de l’incubateur	30°C
Format	Liquid
Composants	10X OPTIZYME™ Buffer 4
Type de produit	SmaI
Site de coupe	CCC.GGG
pH	7.4
Concentration	10 U/μL
À utiliser avec (application)	>90% of pUC19 DNA fragments can be ligated and re-cut after 50-fold over-digestion with SmaI

OPTIZYME™ DdeI (HpyF31), Fisher BioReagents™

Prix et disponibilité
Spécifications

Tampon de stockage	10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Température de l’incubateur	37°C
Format	Liquid
Composants	25 to 50% Water, >50% Glycerin
Site de coupe	C.TNAG
pH	7.4
Concentration	10 U/μL
Contenu et stockage	Keep container tightly closed
À utiliser avec (application)	>95% of DNA fragments can be ligated and re-cut after 50-fold over-digesiton with DdeI

OPTIZYME™ ClaI (Bsu15I), Fisher BioReagents™

Prix et disponibilité
Spécifications

Tampon de stockage	10mM Tris HCl (pH 8 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol
Température de l’incubateur	37°C
Format	Liquid
Composants	>50% Water, 25 to 50% Glycerin
Type de produit	ClaI (Bsu15I)
Site de coupe	AT.CGAT
pH	7.4
Concentration	10 U/μL
Contenu et stockage	Keep container tightly closed

Thermo Scientific™ KpnI Buffer (10X)

Optimize digestions with KpnI restriction enzyme using this buffer, premixed with BSA for enhanced stability.

Prix et disponibilité

Thermo Scientific™ AarI-AjiI-Bpu10I-ScaI-PasI Buffer (10X)

Optimize digestions with AarI, AjiI, Bpu10I, ScaI, and PasI restriction enzymes using this buffer.

Prix et disponibilité

Thermo Scientific™ PasI (10 U/µL)

Cut at CC^CWGGG sites with PasI restriction enzyme, which performs best at 55°C in its own unique buffer.

Prix et disponibilité

Thermo Scientific™ Ecl136II (EcoICRI) (10 U/µL)

Cut at GAG^CTC sites with Ecl136II (EcoICRI) restriction enzyme, which performs best at 37°C in its own unique buffer (Isoschizomers: Eco53kI, EcoICRI, Psp124BI, SstI).

Prix et disponibilité

Thermo Scientific™ FastDigest AatII

Cut at GACGT^C sites with Thermo Scientific™ FastDigest™ AatII, which performs best at 37°C in 5-15 minutes using universal FastDigest™ Buffer (Isoschizomers: ZraI).

Prix et disponibilité

Outils à oligos et ARNi

Outils à oligos et ARNi

Restriction Enzymes

Restriction Enzymes

Résultats de la recherche filtrée

Filtrer par