Crosslinking, Labeling and Protein Modification
Crosslinking, Labeling and Protein Modification
MilliporeSigma™ Calbiochem™ Cleland’s Reagent, Molecular Biology Grade
Protective agent for SH groups
MilliporeSigma™ Calbiochem™ EDTA, Disodium Salt, Dihydrate, Molecular Biology Grade
Application in Molecular Biology
MilliporeSigma™ Calbiochem™ EDTA, 0.5 M, pH 8.0, Molecular Biology Grade, DEPC-Treated
Suitable for use in molecular biology applications
Thermo Scientific™ Pierce™ 2-Mercaptoethanol
Pure, liquid (equals 14M), beta-mercaptoethanol (bME, 2BME, 2-ME, b-mer), CAS 60-24-2; thiol reducing agent for cleaving protein disulfide bonds (cystines cysteines).
Thermo Scientific™ Guanidine-HCl
Pure, crystalline guanidium hydrochloride (GdnHCl, GuHCl), CAS 50-01-1; denaturant for protein biology methods (elution, solubilization, refolding, storage).
Molecular Probes™ 1-pyrenebutanoic acid, succinimidyl ester
1-pyrenebutanoic acid, succinimidyl ester
Thermo Scientific™ CT(PEG)12 Carboxy-PEG-Thiol Compound
CT(PEG)n: carboxylic acid and sulfhydryl compound with a 12-unit polyethylene glycol (PEG) spacer; carboxylate and pegylate quantum dots, gold surfaces, silver.
Thermo Scientific™ MT(PEG)4 Methyl-PEG-Thiol Compound
MT(PEG)n: methyl and sulfhydryl compound with a 4-unit polyethylene glycol (PEG) spacer; methylate and pegylate quantum dots, gold surfaces, silver.
Thermo Scientific™ Pierce™ Sulfhydryl Addition Kit
Modify protein primary amines to create free sulfhydryl groups for labeling, crosslinking or immobilization techniques; complete kit with SATA reagent and hydroxylamine.
Thermo Scientific™ WELQut Protease (5 U/µL)
Thermo Scientific™ WELQut Protease is a highly specific serine protease cleaving outside the WELQX recognition motif to remove N-terminal protein fusion tags.
Thermo Scientific™ EZ-Link™ Alkoxyamine-PEG4-Biotin
Biotinylate glycoproteins (aldehydes of oxidized sugars) with a mid-length (27.0A), pegylated biotinylation reagent containing a 4-unit PEG spacer for length and solubility.
Thermo Scientific™ EZ-Link™ Alkoxyamine-PEG12-Biotin
Biotinylate glycoproteins (aldehydes of oxidized sugars) with a very long (55.4A), pegylated biotinylation reagent containing a 12-unit PEG spacer for length and solubility.
Thermo Scientific™ Pierce™ Hydroxylamine-HCl
NH2OH (CAS 7803-49-8) to cleave Asn-Gly peptide bonds or deprotect sulfhydryls following S-acetylthioacetate modification with SATA reagent.
Thermo Scientific™ EZ-Link™ Amine-PEG11-Biotin
Biotinylate proteins or surfaces using EDC or other amine-reactive crosslinkers with this long (53.2A) biotinylation reagent containing an 11-unit polyethylene glycol spacer.
Thermo Scientific™ EZ-Link™ NHS-PEG Solid-Phase Biotinylation Kit - 1mL Column
Biotinylate protein amines with greater control over the amine-labeling reaction and antibody cleanup process using this column-based method of antibody biotinylation.
Thermo Scientific™ Sulfo-SHPP (Water-Soluble Bolton-Hunter Reagent)
Add tyrosine (tyrosyl) groups to proteins to increase the number of iodine-125 labeling (iodination) sites using an amine-reactive, NHS ester compound.
Thermo Scientific™ EZ-Link™ NHS-Desthiobiotin
Label intracellular proteins for affinity purification with streptavidin beads
Thermo Scientific™ Sulfo-GMBS (N-γ-maleimidobutyryl-oxysulfosuccinimide ester)
Crosslink amines to sulfhydryls with this short, water-soluble crosslinker composed of sulfo-NHS-ester and maleimide groups at ends of an aliphatic, 6-atom (7.3A) spacer arm.
Thermo Scientific™ iodoTMTsixplex™ Isobaric Mass Tagging Kit
Perform measurement of protein and peptide cysteine modifications (S-nitrosylation, oxidation and disulfide bridges) by multiplex quantitative mass spectrometry.
Invitrogen™ pHrodo™ iFL Green Microscale Protein Labeling Kit
The pHrodo iFL Microscale Protein Labeling Kit provides the reagents needed to easily label 3 microscale purified protein or antibody samples (20–100 µg) with pHrodo iFL Red or Green dye.
Invitrogen™ pHrodo™ iFL Red Microscale Protein Labeling Kit
The pHrodo iFL Microscale Protein Labeling Kit provides the reagents needed to easily label 3 microscale purified protein or antibody samples (20–100 µg) with pHrodo iFL Red or Green dye.
Thermo Scientific™ Immobilized Anti-TMT™ Antibody Resin
Thermo Scientific™ Pierce™ Anti-TMT Antibody Resin enables selective enrichment of peptides in complex samples that have been labeled with Tandem Mass Tag™ Reagents.
Thermo Scientific™ DTSSP (3,3'-dithiobis(sulfosuccinimidyl propionate))
Crosslink amines to amines with Sulfo-DSP; composed of sulfo-NHS-ester groups and disulfide spacer arm (12.0A) that is cleavable with reducing agents.
Thermo Scientific™ TMT™ Elution Buffer
Thermo Scientific™ TMT™ Elution Buffer is for use with Thermo Scientific™ Immobilized Anti-TMT™ Resin to enrich samples for peptides that have been chemically labeled with Tandem Mass Tag™ (TMT™) Reagents.
Invitrogen™ Click-iT™ Protein Enrichment Kit, for click chemistry capture of azide-modified proteins
Capture of click-chemistry-based azide-modified proteins
Thermo Scientific™ iodoTMTzero™ Label Reagent, 5 x 0.2 mg
Perform measurement of protein and peptide cysteine modifications (S-nitrosylation, oxidation and disulfide bridges) by multiplex quantitative mass spectrometry.
Thermo Scientific™ LanthaScreen™ Thiol Reactive Tb Chelate
LanthaScreen™ Thiol Reactive Tb Chelate
Molecular Probes™ Biotin Alkyne (PEG4 carboxamide-Propargyl Biotin)
biotin alkyne (PEG4 carboxamide-propargyl biotin)
Molecular Probes™ Biotin Azide (PEG4 carboxamide-6-Azidohexanyl Biotin)
biotin azide (PEG4 carboxamide-6-azidohexanyl biotin)