Enzymes and Inhibitors
Enzymes and Inhibitors
Restriction enzymes, modifying enzymes, buffering solutions, inhibitors, and substrates for use in clinical, research, and general laboratory procedures.
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1
| Tampon de stockage | 20mM Tris HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol |
|---|---|
| Format | Liquid |
| Composants | 25 to 50% Water, >50% Glycerin |
| Type de produit | NotI |
| Site de coupe | GC.GGCCGC |
| pH | 7.4 |
| Concentration | 10 U/μL |
| Contenu et stockage | Keep container tightly closed |
| À utiliser avec (application) | >90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Not I |
2
| Tampon de stockage | 10mM Tris HCl (pH 7.5 at 25°C), 250mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA, 50% Glycerol |
|---|---|
| Température de l’incubateur | 37°C |
| Format | Liquid |
| Composants | 25 to 50% Water, >50% Glycerin, 1 to 2.5% Potassium Chloride |
| Type de produit | HindIII |
| Site de coupe | A.AGCTT |
| Concentration | 10 U/μL |
| Contenu et stockage | Keep container tightly closed |
| À utiliser avec (application) | >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with HaeIII |
3
| Tampon de stockage | 10mM Tris HCl (pH 7.4 at 25°C), 200mM NaCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol |
|---|---|
| Température de l’incubateur | 37°C |
| Format | Liquid |
| Composants | 25 to 50% Water, >50% Glycerin, 1 to 2.5% Sodium Chloride |
| Type de produit | BamHI |
| Site de coupe | G.GATCC |
| pH | 7.4 |
| Concentration | 10 U/μL |
| Contenu et stockage | Keep container tightly closed |
| À utiliser avec (application) | >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BamHI |
4
| Tampon de stockage | 10mM Potassium Phosphate (pH 7.4 at 25°C), 300mM NaCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 0.15% Triton™ X-100, 50% Glycerol |
|---|---|
| Température de l’incubateur | 37°C |
| Format | Liquid |
| Type de produit | EcoRI |
| Site de coupe | G.AATTC |
| pH | 7.4 |
| Contenu et stockage | Keep container tightly closed |
| À utiliser avec (application) | >90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with EcoRI |
5
| Tampon de stockage | 10mM Tris HCl (pH 8.2 at 25°C), 200mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol |
|---|---|
| Température de l’incubateur | 37°C |
| Format | Liquid |
| Composants | 25 to 50% Water, >50% Glycerin, 1 to 2.5% Sodium Chloride |
| Type de produit | BglII |
| Site de coupe | A.GATCT |
| pH | 7.4 |
| Concentration | 10 U/μL |
| Contenu et stockage | Keep container tightly closed |
| À utiliser avec (application) | >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BglII |
6
| Tampon de stockage | 10mM Tris HCl (pH 7.4), 100mM KCl, 7mM 2-mercaptoethano, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol |
|---|---|
| Température de l’incubateur | 37°C |
| Format | Liquid |
| Composants | 10X OPTIZYME™ Buffer 4 |
| Type de produit | XbaI |
| Site de coupe | T.CTAGA |
| pH | 7.4 |
| À utiliser avec (application) | Indicates ligation efficiency of DNA fragments generated by digestion with the Restriction Enzyme |
7
| Tampon de stockage | 10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 50% Glycerol |
|---|---|
| Température de l’incubateur | 30°C |
| Format | Liquid |
| Composants | 10X OPTIZYME™ Buffer 4 |
| Type de produit | SmaI |
| Site de coupe | CCC.GGG |
| pH | 7.4 |
| Concentration | 10 U/μL |
| À utiliser avec (application) | >90% of pUC19 DNA fragments can be ligated and re-cut after 50-fold over-digestion with SmaI |
8
| Tampon de stockage | 10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol |
|---|---|
| Température de l’incubateur | 37°C |
| Format | Liquid |
| Composants | 25 to 50% Water, >50% Glycerin |
| Site de coupe | C.TNAG |
| pH | 7.4 |
| Concentration | 10 U/μL |
| Contenu et stockage | Keep container tightly closed |
| À utiliser avec (application) | >95% of DNA fragments can be ligated and re-cut after 50-fold over-digesiton with DdeI |
9
| Tampon de stockage | 10mM Tris HCl (pH 8 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol |
|---|---|
| Température de l’incubateur | 37°C |
| Format | Liquid |
| Composants | >50% Water, 25 to 50% Glycerin |
| Type de produit | ClaI (Bsu15I) |
| Site de coupe | AT.CGAT |
| pH | 7.4 |
| Concentration | 10 U/μL |
| Contenu et stockage | Keep container tightly closed |