Chromatography

The Fisher Scientific channel offers a full range of chromatography products to suit your specific needs. We cover each step of your workflow, providing everything from vials and closures, solvents, and standards to liquid and gas chromatography columns and thin layer chromatography paper — all from trusted suppliers and brands.

The term chromatography describes a variety of techniques that are used to separate a substance or mixture into its components. It is based on the principle that the weight or size of the component will affect the speed at which it travels through another medium. When the components move at different rates, they naturally create a separation.

Chromatography can be both preparative and analytical. It can be used to process a substance before it is analyzed or used as an analytic method itself to measure the amount or proportion of a component in the substance.

The choice of chromatography methods depends on the specific application and substance, as well as the polarity of the media and solvent. Commonly used chromatography methods include:

  • Paper chromatography
    • Paper is dotted with a substance
    • One end of the paper is immersed in solvent, which carries the components with it as it travels through the paper
  • Thin layer chromatography (TLC)
    • Similar to paper chromatography, but uses a glass plate coated with adsorbent as a medium
    • Used for drug levels and water purity testing
  • Gas chromatography
    • Requires that the sample be heated to a gaseous state
    • Well-suited for petrochemical, environmental, and industrial chemical research and testing
  • Liquid chromatography
    • Includes High Performance Liquid Chromatography (HPLC), one of the most frequently used research and analytical techniques
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Gas Chromatography

Gas chromatography (GC) is used to separate chemicals based on their volatility, or the ease with which they evaporate into a gas. Samples are injected through a heated injection port into an inert carrier gas such as helium, argon or nitrogen. This vaporized mobile phase moves through either a packed column with a solid support or a capillary column with a liquid (high-boiling polymer) stationary phase; columns are housed in a temperature-controlled oven to facilitate movement of the molecules. Sample components are separated based on interactions with the stationary phase as they pass through the column.

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Sample Preparation

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Sample Handling

gas chromatography columns

High-performance Liquid Chromatography

High-performance liquid chromatography (HPLC) is a technique used to separate a mixture of compounds with the purpose of identifying and analyzing the components. The mixture is injected into a polar solvent, or eluent; this mobile phase is then pumped at high pressure through a column. The column, or stationary phase, is packed with nonpolar or hydrophobic silica particles bonded with alkyl chains. As the mobile phase passes through the column, the differences in travel rate, or retention time, of the components facilitates separation. Components are detected by ultraviolet absorption; the absorption spectrum is captured as a chromatogram, which is then used for analysis.

bottles of chemical solvents
thermo scientific sola spe cartridges
high performance liquid chromatography columns

Sample Prep

Sample preparation is a key component in driving consistent separations, sample protection and ensuring long column and instrument life. Sample prep techniques can be as simple as chromatography specific syringe filters all the way to solid phase extraction, depending on your analysis needs.

bottles of chemical solvents

Sample Preparation

solid phase extraction plates

Sample Handling

liquid chromatography columns
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