Crosslinking and Protein Modification

Thermo Scientific™ Bond-Breaker™ TCEP Solution, Neutral pH

Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.

Fisherbrand™ UV Crosslinker with Adjustable Height

Provides a safe, time-efficient solution with a controlled amount of ultraviolet radiation.

Thermo Scientific™ Sulfo-NHS (N-hydroxysulfosuccinimide), No-Weigh™ Format

Prepare amine-reactive sulfo-NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysulfosuccinimide, CAS 106627-54-7.

Thermo Scientific™ Pierce™ DSP, No-Weigh™ Format

Crosslink amines to amines with dithiobis succinimidyl propionate, composed of NHS-ester groups and disulfide-containing, 12.0A spacer arm and cleavable with reducing agents.

Thermo Scientific™ NHS (N-hydroxysuccinimide)

Prepare amine-reactive NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysuccinimide, CAS 6066-82-6.

Thermo Scientific™ Pierce™ EDC, No-Weigh™ Format

Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.

Thermo Scientific™ BS3 (bis(sulfosuccinimidyl)suberate)

Crosslink amines to amines with BS3; composed of sulfo-NHS-ester groups separated by an 11.4A, water-soluble spacer arm that is useful for cell-surface protein crosslinking.

Thermo Scientific™ Sulfo-SIAB (sulfosuccinimidyl (4-iodoacetyl)aminobenzoate)

Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker composed of sulfo-NHS-ester and iodoacetyl (haloacetyl) groups at ends of a 9-atom spacer arm.

Thermo Scientific™ 8M Guanidine-HCl Solution

Ready-to-use, 8M solution of guanidinium hydrochloride (GdnCl, GuHCl), CAS 50-01-1; dilute and pH-adjust to any concentration for protein biology methods.

Thermo Scientific™ Pierce™ TCEP-HCl, No-Weigh™ Format

Reduce protein and peptide disulfide bonds using this thiol-free, pure, crystalline tris (2-carboxyethyl) phosphine hydrochloride, TCEP (CAS 5961-85-3).

Thermo Scientific™ Pierce™ DTT (Dithiothreitol)

Reduce disulfides in biological buffers and sample loading buffers with pure, crystalline dithiothreitol (DTT), the most popular protein disulfide reducing agent.

Thermo Scientific™ SMPH (Succinimidyl 6-((beta-maleimidopropionamido)hexanoate))

Crosslink amines to sulfhydryls with this long crosslinker composed of NHS-ester and maleimide groups at ends of an aliphatic, 12-atom (14.2A) spacer arm.

Thermo Scientific™ SDA (NHS-Diazirine) (succinimidyl 4,4'-azipentanoate)

Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).

Thermo Scientific™ DTSSP (3,3'-dithiobis(sulfosuccinimidyl propionate))

Crosslink amines to amines with Sulfo-DSP; composed of sulfo-NHS-ester groups and disulfide spacer arm (12.0A) that is cleavable with reducing agents.

Spectroline™ Microprocessor-Controlled UV Crosslinkers

Produce publication-quality results every time

Thermo Scientific™ Pierce™ Premium Grade EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride)

Optimize applications where product integrity and risk minimization are paramount with our highest quality formulation of this popular carbodiimide crosslinker.

Thermo Scientific™ Pierce™ Mercaptoethylamine-HCl

Pure, crystalline 2-aminoethanethiol (2-MEA-HCl, also called cysteamine-HCl), CAS 156-57-0; selectively reduces antibody hinge-region disulfide bonds.

Thermo Scientific™ Pierce™ NEM (N-ethylmaleimide)

Small, sulfhydryl-reactive (maleimide) compound to permanently block reduced cysteines of proteins or peptides to prevent disulfide bond formation.

Thermo Scientific™ DSS (disuccinimidyl suberate), No-Weigh™ Format

Crosslink amines to amines with disuccinimidyl suberate composed of NHS-ester groups with a membrane-permeable, mid-length spacer for intracellular protein crosslinking.

Thermo Scientific™ Pierce™ Citraconic Anhydride

Reversibly cap lysines and other primary amines with carboxyl groups using this amine-reactive compound; stable at pH 7 but hydrolyzes (decouples) at pH 3 to 4 (CAS 616-02-4).

Thermo Scientific™ Sulfo-LC-SPDP (sulfosuccinimidyl 6-(3'-(2-pyridyldithio)propionamido)hexanoate)

Crosslink amines to sulfhydryls with this water-soluble sulfo-NHS-ester and pyridyldithiol groups on 10-atom spacer arms to form cleavable disulfide bonds with cysteines.

Thermo Scientific™ EMCH (N-ε-maleimidocaproic acid hydrazide)

Crosslink sulfhydryls to periodate-treated glycoprotein sugars with this mid-length (11.8A) crosslinker containing maleimide and hydrazide groups.

Thermo Scientific™ Pierce™ Premium Grade TCEP-HCl

Optimize applications where product integrity and risk minimization are paramount with our highest quality formulation of this disulfide reducing agent.

Thermo Scientific™ MBS (m-maleimidobenzoyl-N-hydroxysuccinimide ester)

Crosslink amines to sulfhydryls with this short crosslinker composed of NHS-ester and maleimide groups at ends of an aromatic, 6-atom (7.3A) spacer arm.

MilliporeSigma™ Calbiochem™ Trehalose, Dihydrate

Non-reducing disaccharide which yields 2 moles of D-glucose upon acid hydrolysis. Inhibits polyglutamine-mediated protein aggregation both in vivo and in vitro.

Thermo Scientific™ L-Photo-Methionine

Diazirine analogs of leucine and methionine to express proteins in cell culture that will crosslink their protein interactors upon UV-light activation in vivo.

Thermo Scientific™ BS(PEG)9 (PEGylated bis(sulfosuccinimidyl)suberate)

Crosslink amines to amines with these long (21.7A and 35.8A) crosslinkers composed of NHS-ester groups and 5-unit or 9-unit PEG spacer arms that enhance protein solubility.

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