Cell Culture Reagents and Kits

Gibco™ ACK Lysing Buffer DFS Item

Used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow

Cytiva (Formerly GE Healthcare Life Sciences) HyClone™ Penicillin Streptomycin 100X Solution

Used to control/destroy destructive microorganisms such as bacteria

Gibco™ Penicillin-Streptomycin (10,000 U/mL) DFS Item

For prevention of bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria

Gibco™ Trypan Blue Solution, 0.4% DFS Item

Routinely used as a cell stain to assess cell viability using the dye exclusion test

Gibco™ Trypsin-EDTA (0.25%), phenol red DFS Item

Made from trypsin powder, irradiated mixture of proteases derived from porcine pancreas

Gibco™ GlutaMAX™ Supplement DFS Item

An alternative to L-glutamine, with increased stability that improves cell health

Corning™ Matrigel™ GFR Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ GFR Basement Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.

Gibco™ MEM Non-Essential Amino Acids Solution (100X) DFS Item

Used as a growth supplement for cell culture medium, to increase cell growth and viability

Gibco™ Antibiotic-Antimycotic (100X) DFS Item

Anti-mycotic, antibiotic for prevention of cell culture contamination

Cytiva (Formerly GE Healthcare Life Sciences) HyClone™ HEPES Solution

Maintains physiological pH in cell culture, despite changes in carbon dioxide concentration (produced by cellular respiration). HyClone™ HEPES Solution is part of high-performance line of nutritional and growth supplements.

Gibco™ Hygromycin B (50 mg/mL) DFS Item

Water-soluble antibiotic purified from bacterium Streptomyces hydroscopicus

Corning™ Matrigel™ hESC-Qualified Matrix

Compatible with mTeSR™1-devloped by StemCell Technologies. Corning™ Matrigel™ hESC-Qualified Matrix is widely accepted as an alternative substrate for the culture of hES cells as well as human induced pluripotent stem (iPS) cells.

Gibco™ TrypLE™ Express Enzyme (1X), no phenol red DFS Item

Unlike porcine trypsin, free of animal-derived components

Gibco™ Gentamicin (50 mg/mL) DFS Item

Water-soluble antibiotic drug originally purified from the fungus Micromonospora purpurea

Gibco™ Geneticin™ Selective Antibiotic (G418 Sulfate) (50 mg/mL) DFS Item

Produced by bacterium Micromonospora rhodorangea and acts by binding ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells

Gibco™ Penicillin-Streptomycin (5,000 U/mL) DFS Item

For prevention from bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria.

Gibco™ Trypsin-EDTA (0.05%), phenol red DFS Item

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

Corning™ Matrigel™ Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.

Gibco™ Trypsin-EDTA (0.5%), no phenol red DFS Item

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

Gibco™ Amphotericin B DFS Item

Formulation of amphotericin B with sodium deoxycholate as an emulsifying agent to maintain the suspension

Corning™ Cell Culture Phosphate Buffered Saline (1X)

With low endotoxin content. Without calcium and magnesium.

Corning™ Cell Recovery Solution

Allows for the recovery of cells cultured on Corning™ Matrigel™ Basement Membrane Matrix for subsequent biochemical analyses

Gibco™ HAT Supplement (50X) DFS Item

HAT-supplemented medium is suitable for post-fusion selection against unfused or self-fused HGPRT-myeloma cells

Corning™ Penicillin-Streptomycin Solution

Effective against Gram-positive and Gram-negative bacteria. Corning™ Penicillin-Streptomycin Solution is a mix of the antibiotics Penicillin (10,000 IU) and Streptomycin (10,000μg/mL) in a 100-fold working concentration.

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