Protein Extraction and Purification

Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.38 MDL Number: MFCD00036175 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: SDS, Sodium Lauryl Sulfate PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: [Na+].CCCCCCCCCCCCOS([O-])(=O)=O

Triton™ X-100 (Electrophoresis), Fisher BioReagents™

CAS: 9002-93-1 Molecular Formula: C16H26O2 Molecular Weight (g/mol): 250.382 InChI Key: JYCQQPHGFMYQCF-UHFFFAOYSA-N Synonym: Polyethylene Glycol p-tert-Octylphenyl Ether PubChem CID: 5590 IUPAC Name: 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol SMILES: CC(C)(C)CC(C)(C)C1=CC=C(C=C1)OCCO

Thermo Scientific™ Low Protein Binding Collection Tubes (1.5 mL)

Minimize protein sample loss during sample manipulation, especially when there are many processing steps, by using Thermo Scientific™ Pierce™ Low Protein Binding Microcentrifuge Tubes (1.5 mL).

Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)

Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.

Invitrogen™ Magnetic Stand-96 DFS Item

Designed for paramagnetic bead precipitation from standard 96-well, U-bottom microplates and 0.2mL PCR plates without additional accessories

SDS Solution, 20% Sodium Dodecyl Sulfate Solution, Molecular Biology/Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.38 MDL Number: MFCD00036175 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: SDS, Sodium lauryl sulfate PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: [Na+].CCCCCCCCCCCCOS([O-])(=O)=O

MilliporeSigma™ BioPak™ Disposable Ultrafilter

Nuclease-free, pyrogen-free water

Thermo Scientific™ RIPA Lysis and Extraction Buffer

Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent.

Thermo Scientific™ Pierce™ Protease Inhibitor Mini Tablets, EDTA-free

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments

Thermo Scientific™ Pierce™ LAL Chromogenic Endotoxin Quantitation Kit

Accurately measure endotoxin levels (LPS, lipopolysaccharides) in protein, peptide or antibody samples using our Limulus Amebocyte Lysate (LAL) assay.

Thermo Scientific™ Halt™ Protease Inhibitor Cocktail (100X)

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.

Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)

Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.

Thermo Scientific™ Pierce™ Spin Columns - Snap Cap

Perform small-scale affinity methods, such as immunoprecipitation assays with agarose beads using these empty spin columns and cups (5 to 500µL).

Thermo Scientific™ Pierce™ Protein Concentrators PES, 10K MWCO, 0.5 mL

Concentrate and desalt biological samples and perform buffer exchange with disposable polyethersulfone (PES)-membrane ultrafiltration centrifugal devices.

Thermo Scientific™ PMSF Protease Inhibitor

Customize your protease inhibitor cocktail formulation with these small packages of individual protease inhibitor peptides and compounds.

Invitrogen™ NanoOrange™ Protein Quantitation Kit, 200-2,000 assays DFS Item

Sensitive and easy assay for protein quantitation, with detection as low as 10 ng/mL of protein in solution

Cytiva Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

Thermo Scientific™ Pierce™ Microcentrifuge Tubes, 2.0 mL

Perform small-scale affinity methods, such as immunoprecipitation assays with agarose beads using these empty spin columns and cups (5 to 500µL).

Cytiva HiTrap™ Protein A HP Prepacked Columns

Use for purification and isolation of monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants. Cytiva Life Sciences™ HiTrap™ Protein A HP Prepacked Columns are specially designed, together with the medium to provide fast, simple and easy separations in a convenient format.

Cytiva HisTrap™ FF Columns: 16 × 25mm

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 × 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

Cytiva HisTrap™ FF Columns: 5 x 5mL

Optimize purifications of histidine-tagged proteins directly from homogenized, unclarified cells HisTrap™ FF Columns: 5 x 5mL are prepacked with precharged Ni Sepharose™ 6 Fast Flow media.

Invitrogen™ 24 Deep-Well RB Block DFS Item

Designed for experimental protocols that require volumes exceeding the capacity of conventional shallow well plates

Thermo Scientific™ HisPur™ Ni-NTA Resin

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Thermo Scientific™ Lysozyme

Degrade unwanted bacterial cell wall in protein or nucleic acid extracts

Invitrogen™ eBioscience™ 10X RBC Lysis Buffer (Multi-species)

Specially formulated for optimal lysis of erythrocytes in single-cell suspensions of peripheral blood and hematopoietic tissues such as spleen. This buffer can be used for lysis of human, mouse, rat, canine, and non-human primate samples.

MilliporeSigma™ Labscale TFF System Reservoir

Easy to use for sample preparation

Thermo Scientific™ Pierce™ Microcentrifuge Tubes, 1.5 mL

Perform small-scale affinity methods, such as immunoprecipitation assays with agarose beads using these empty spin columns and cups (5 to 500µL).

Cytiva Sera-Mag SpeedBeads™ Carboxyl Magnetic Beads, hydrophobic

Excellent for coupling oligos or proteins directly to the beads. Their “cauliflower-like” structure gives Sera-Mag beads high binding capacity. The carboxyl groups on these beads can be activated by standard EDC/EDAC chemistry to react with free amino groups, forming amide bonds.

Thermo Scientific™ M-PER™ Mammalian Protein Extraction Reagent

Isolate total protein from cultured mammalian cells with this highly efficient lysis and extraction reagent.

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