Protein Biology

Invitrogen™ Subcloning Efficiency™ DH5α Competent Cells DFS Item

Economical for routine subcloning procedures or applications where starting DNA is not limiting

Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: SDS PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]

Triton™ X-100 (Electrophoresis), Fisher BioReagents™

CAS: 9002-93-1 Molecular Formula: C16H26O2 Molecular Weight (g/mol): 250.382 InChI Key: JYCQQPHGFMYQCF-UHFFFAOYSA-N Synonym: Polyethylene Glycol p-tert-Octylphenyl Ether PubChem CID: 5590 IUPAC Name: 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol SMILES: CC(C)(C)CC(C)(C)C1=CC=C(C=C1)OCCO

MilliporeSigma™ Immobilon™-P PVDF Transfer Membranes

Optimized for binding proteins transferred from a variety of gel matrices. Immobilon™-P PVDF Transfer Membranes have superior handling, staining, and resistance than nitrocellulose. For use with Western blots and protein sequencing. Available in sheets or rolls.

Thermo Scientific™ Pierce™ BCA™ Protein Assay

Green-to-blue (A562nm), precise, detergent-compatible assay reagent to measure total protein concentration vs. protein standard.

Thermo Scientific™ Pierce™ Bovine Serum Albumin Standards

High-quality, calibrated BSA solutions (2mg/mL) to serially dilute to create protein assay standard curves to accurately measure protein concentration.

MilliporeSigma™ BioPak™ Disposable Ultrafilter

Nuclease-free, pyrogen-free water from any MilliporeSigma™ Ultrapure™ water system

Thermo Scientific™ 6X DNA Loading Dye

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.

Invitrogen™ IPTG DFS Item

Inducer of β-galactosidase activity in bacteria and is suitable for use with X-gal or bluo-gal to detect lac gene activity in cloning procedures.

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, without competent cells DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (“TOPO Cloning”) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for subcloning

Thermo Scientific™ Pierce™ LAL Chromogenic Endotoxin Quantitation Kit

Accurately measure endotoxin levels (LPS, lipopolysaccharides) in protein, peptide or antibody samples using our Limulus Amebocyte Lysate (LAL) assay.

Thermo Scientific™ Pierce™ Methanol-free Formaldehyde Ampules

Vials of high-quality, methanol-free formaldehyde, a general IHC fixative and reversible amine-crosslinking agent for protein and nucleic acid samples.

Invitrogen™ One Shot™ Stbl3™ Chemically Competent E. coli DFS Item

Designed especially for cloning direct repeats found in lentiviral expression vectors

Thermo Scientific™ X-Gal Solution, ready-to-use

Effectively screen colonies with X-Gal, an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal, forming an intense blue precipitate.

Invitrogen™ Magnetic Stand-96 DFS Item

Designed for paramagnetic bead precipitation from standard 96-well, U-bottom microplates and 0.2mL PCR plates without additional accessories

Invitrogen™ One Shot™ TOP10 Chemically Competent E. coli, High Copy Plasmid DFS Item

Ideal for high-efficiency cloning and plasmid propagation

Thermo Scientific™ Pierce™ Coomassie (Bradford) Protein Assay

Brown-to-purple (A595nm), ready-to-use, classic reducing agent-compatible assay reagent to measure total protein concentration vs. protein standard.

GE Healthcare PD SpinTrap™ G-25 Desalting Columns

Use for convenient and fast small-scale cleanup of proteins and other large biomolecules (>5000 Mr).  GE Healthcare Life Sciences™ PD SpinTrap™ G-25 Desalting Columns provide the option of running multiple samples in parallel.

Gibco™ Freestyle™ 293 Expression Medium DFS Item

An animal origin-free, serum-free, protein-free formulation, allowing for easier purification

GE Healthcare Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity.  GE Healthcare Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

Thermo Scientific™ SuperSignal™ West Femto Chemiluminescent Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

Thermo Scientific™ Pierce™ TMB Substrates

Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.

Invitrogen™ Molecular Probes™ MitoTracker™ Green FM DFS Item

Green-fluorescent mitochondrial stain

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments

Thermo Scientific™ HisPur™ Ni-NTA Resin

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Invitrogen™ Molecular Probes™ MitoTracker™ Red CMXRos DFS Item

For use with microarray, flow cytometry and fluorescence microscopy

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