Protein Biology

Invitrogen™ Subcloning Efficiency™ DH5α Competent Cells DFS Item

Economical for routine subcloning procedures or applications where starting DNA is not limiting

Thermo Scientific™ Pierce™ BCA Protein Assay Kit

Green-to-blue (A562nm), precise, detergent-compatible assay reagent to measure total protein concentration vs. protein standard.

Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: SDS, Sodium Lauryl Sulfate PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]

Triton™ X-100 (Electrophoresis), Fisher BioReagents™

CAS: 9002-93-1 Molecular Formula: C16H26O2 Molecular Weight (g/mol): 250.382 InChI Key: JYCQQPHGFMYQCF-UHFFFAOYSA-N Synonym: Polyethylene Glycol p-tert-Octylphenyl Ether PubChem CID: 5590 IUPAC Name: 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol SMILES: CC(C)(C)CC(C)(C)C1=CC=C(C=C1)OCCO

MilliporeSigma™ BioPak™ Disposable Ultrafilter

Nuclease-free, pyrogen-free water

Thermo Scientific™ Pierce™ 16% Formaldehyde (w/v), Methanol-free

Vials of high-quality, methanol-free formaldehyde, a general IHC fixative and reversible amine-crosslinking agent for protein and nucleic acid samples.

MilliporeSigma™ Immobilon™-P PVDF Transfer Membranes

Optimized for binding proteins transferred from a variety of gel matrices. Immobilon™-P PVDF Transfer Membranes have superior handling, staining, and resistance than nitrocellulose. For use with Western blots and protein sequencing. Available in sheets or rolls.

Invitrogen™ MitoTracker™ Green FM - Special Packaging DFS Item

Green-fluorescent mitochondrial stain

Thermo Scientific™ Pierce™ Bovine Serum Albumin Standard Ampules, 2 mg/mL

High-quality, calibrated BSA solutions (2mg/mL) to serially dilute to create protein assay standard curves to accurately measure protein concentration.

Thermo Scientific™ DNA Gel Loading Dye (6X)

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.

MilliporeSigma™ Luminata™ Western HRP Chemiluminescence Substrates

Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns

Invitrogen™ eBioscience™ 7-AAD Viability Staining Solution

Ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis. Can be used in combination with PE- and FITC-conjugated antibodies in 2-color analysis. The advantage of 7-AAD over PI is that there is minimal spectral overlap between these emissions.

Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

Thermo Scientific™ Pierce™ Coomassie Plus (Bradford) Assay Kit

Brown-to-purple (A595nm), ready-to-use, fast reducing agent-compatible assay reagent to measure total protein concentration vs. protein standard.

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity.  Cytiva Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

Spectrum™ Spectra/Por™ 4 RC Dialysis Membrane Tubing 12,000 to 14,000 Dalton MWCO

Ideal for molecular separation, protein binding studies, electrolyte removal, desalting.

Thermo Scientific™ Micro BCA™ Protein Assay Kit

Special 3-component version of our popular BCA Reagents to measure total protein concentration of dilute protein solutions (0.5 to 20 micrograms/mL).

Thermo Scientific™ SnakeSkin™ Dialysis Tubing, 3.5K MWCO, 35 mm

Perform desalting and buffer exchange for 10 to 100mL samples with this 3500-MW cut-off (MWCO), ready-to-use form of traditional dialysis membrane tubing.

Thermo Scientific™ Pierce™ TMB Substrate Kit

Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.

Promega ENLITEN™ ATP Assay System DFS Item

The ENLITEN ATP Assay System is used to measure ATP levels for the indirect detection of biocontamination.

Thermo Scientific™ Pierce™ Coomassie (Bradford) Protein Assay Kit

Brown-to-purple (A595nm), ready-to-use, classic reducing agent-compatible assay reagent to measure total protein concentration vs. protein standard.

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ ECL Select™ Western Blotting Detection Reagent

Use as a high sensitivity reagent for chemiluminescent Western blotting detection. Cytiva Life Sciences™ Amersham™ ECL Select™ Western Blotting Detection Reagent  provides highly intense signal output, for detection of medium to very low protein levels.

Invitrogen™ NP40 Cell Lysis Buffer DFS Item

Suitable for preparation of cell extracts

Invitrogen™ NuPAGE™ MES SDS Running Buffer (20X) DFS Item

Made with high-purity reagents and are strictly quality controlled

Cytiva (Formerly GE Healthcare Life Sciences) Disposable PD-10 Desalting Columns

Use for desalting and buffer exchange, as well as removal of low-molecular weight compounds. Cytiva Life Sciences™ Disposable PD-10 Desalting Columns provide fast, high-recovery.

Thermo Scientific™ Low Protein Binding Collection Tubes (1.5 mL)

Minimize protein sample loss during sample manipulation, especially when there are many processing steps, by using Thermo Scientific™ Pierce™ Low Protein Binding Microcentrifuge Tubes (1.5 mL).

Invitrogen™ Magnetic Stand-96 DFS Item

Designed for paramagnetic bead precipitation from standard 96-well, U-bottom microplates and 0.2mL PCR plates without additional accessories

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