Invitrogen™ Subcloning Efficiency™ DH5α Competent Cells
Economical for routine subcloning procedures or applications where starting DNA is not limiting
Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagents™
CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: SDS PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]
Triton™ X-100 (Electrophoresis), Fisher BioReagents™
CAS: 9002-93-1 Molecular Formula: C16H26O2 Molecular Weight (g/mol): 250.382 InChI Key: JYCQQPHGFMYQCF-UHFFFAOYSA-N Synonym: Polyethylene Glycol p-tert-Octylphenyl Ether PubChem CID: 5590 IUPAC Name: 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol SMILES: CC(C)(C)CC(C)(C)C1=CC=C(C=C1)OCCO
MilliporeSigma™ Immobilon™-P PVDF Transfer Membranes
Optimized for binding proteins transferred from a variety of gel matrices. Immobilon™-P PVDF Transfer Membranes have superior handling, staining, and resistance than nitrocellulose. For use with Western blots and protein sequencing. Available in sheets or rolls.
Thermo Scientific™ Pierce™ BCA™ Protein Assay
Green-to-blue (A562nm), precise, detergent-compatible assay reagent to measure total protein concentration vs. protein standard.
Thermo Scientific™ Pierce™ Bovine Serum Albumin Standards
High-quality, calibrated BSA solutions (2mg/mL) to serially dilute to create protein assay standard curves to accurately measure protein concentration.
MilliporeSigma™ BioPak™ Disposable Ultrafilter
Nuclease-free, pyrogen-free water from any MilliporeSigma™ Ultrapure™ water system
Thermo Scientific™ 6X DNA Loading Dye
Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.
Inducer of β-galactosidase activity in bacteria and is suitable for use with X-gal or bluo-gal to detect lac gene activity in cloning procedures.
Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, without competent cells
Provides a highly efficient, 5-minute, one-step cloning strategy (“TOPO Cloning”) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for subcloning
Thermo Scientific™ Pierce™ LAL Chromogenic Endotoxin Quantitation Kit
Accurately measure endotoxin levels (LPS, lipopolysaccharides) in protein, peptide or antibody samples using our Limulus Amebocyte Lysate (LAL) assay.
Thermo Scientific™ Pierce™ Methanol-free Formaldehyde Ampules
Vials of high-quality, methanol-free formaldehyde, a general IHC fixative and reversible amine-crosslinking agent for protein and nucleic acid samples.
Invitrogen™ One Shot™ Stbl3™ Chemically Competent E. coli
Designed especially for cloning direct repeats found in lentiviral expression vectors
Thermo Scientific™ X-Gal Solution, ready-to-use
Effectively screen colonies with X-Gal, an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal, forming an intense blue precipitate.
Invitrogen™ Magnetic Stand-96
Designed for paramagnetic bead precipitation from standard 96-well, U-bottom microplates and 0.2mL PCR plates without additional accessories
Invitrogen™ One Shot™ TOP10 Chemically Competent E. coli, High Copy Plasmid
Ideal for high-efficiency cloning and plasmid propagation
Thermo Scientific™ Pierce™ Coomassie (Bradford) Protein Assay
Brown-to-purple (A595nm), ready-to-use, classic reducing agent-compatible assay reagent to measure total protein concentration vs. protein standard.
GE Healthcare PD SpinTrap™ G-25 Desalting Columns
Use for convenient and fast small-scale cleanup of proteins and other large biomolecules (>5000 Mr). GE Healthcare Life Sciences™ PD SpinTrap™ G-25 Desalting Columns provide the option of running multiple samples in parallel.
Gibco™ Freestyle™ 293 Expression Medium
An animal origin-free, serum-free, protein-free formulation, allowing for easier purification
GE Healthcare Amersham™ ECL™ Prime Western Blotting Detection Reagent
Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity. GE Healthcare Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.
Thermo Scientific™ SuperSignal™ West Femto Chemiluminescent Substrate
Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.
Thermo Scientific™ Pierce™ TMB Substrates
Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.
Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets
Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments
Thermo Scientific™ HisPur™ Ni-NTA Resin
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
Invitrogen™ Molecular Probes™ DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride)
Nuclear and chromosome counterstain