Enzymes and Inhibitors

Thermo Scientific™ RNase A, DNase and protease-free (10 mg/mL)

Specifically degrade single-stranded RNA at C and U residues with the endoribonuclease RNase A.

Thermo Scientific™ Bovine Serum Albumin (BSA)

Stabilize enzymes, increase PCR yields, and prevent adhesion of enzymes with Thermo Scientific™ Bovine Serum Albumin.

Thermo Scientific™ DNase I, RNase-free (1 U/µL)

Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.

Thermo Scientific™ FastDigest BamHI

Cut at G^GATCC sites with Thermo Scientific™ FastDigest™ BamHI restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Promega Trypsin Gold, Mass Spectrometry Grade DFS Item

Trypsin Gold, Mass Spectrometry Grade, provides maximum specificity for mass spectrometry.

Thermo Scientific™ Proteinase K, recombinant, PCR grade

Degrade proteins even in the presence of detergents with Proteinase K, a broad-range endolytic protease widely used for digestion of proteins in nucleic acid preparations.

Thermo Scientific™ AarI (2 U/µL)

Cut at CACCTGC(4/8)^ sites with AarI restriction enzyme, which performs best at 37°C in its own unique (+oligo) buffer.

Thermo Scientific™ Tango Buffer (10X)

Ensure the optimum reaction conditions for restriction enzymes with Thermo Scientific™ 10X Buffer Tango, premixed with BSA for enhanced stability.

Promega RQ1 RNase-Free DNase DFS Item

RQ1 (RNA Qualified) RNase-Free DNase is a DNase I that degrades both double-stranded and signle-stranded DNA.

Promega Proteinase K DFS Item

Serine protease that exhibits a very broad cleavage specificity. Useful in many molecular biology applications.

Invitrogen™ Ambion™ DNase I (RNase-free) DFS Item

Nonspecific endonuclease that degrades double- and single-stranded DNA and chromatin

Thermo Scientific™ Buffer R (10X)

Ensure the optimum reaction conditions for restriction enzymes with Thermo Scientific™ 10X Buffer R, premixed with BSA for enhanced stability.

Invitrogen™ DNase I, Amplification Grade DFS Item

Purified and tested for nondetectable levels of RNase contamination

MP Biomedicals™ alpha-Amylase from Bacillus subtilis

Liquefying diastase supplied as dry, free-flowing powder with high uniformity and storage stability

Invitrogen™ TURBO DNA-free™ Kit DFS Item

Ideal tool for clearing residual DNA

Thermo Scientific™ FastDigest DpnI

Cut at Gm6A^TC sites with Thermo Scientific™ FastDigest™ DpnI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: MalI).

Thermo Scientific™ RiboLock RNase Inhibitor (40 U/µL)

RiboLock RNase Inhibitor specifically inhibits the activity of RNases A, B, and C, protecting RNA under a variety of reaction conditions.

Thermo Scientific™ FastAP Thermosensitive Alkaline Phosphatase (1 U/µL)

Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase .

MP Biomedicals™ Pepsin, MP Biomedicals™

Used to digest proteins and in the preparation of Fab fragments from antibodies. Pepsin, MP Biomedicals is an acid protease that contains a proteolytic enzyme with a &Primelcathepsin&Primel component that has milk curdling activity. It has a broad range of substrate

Promega Proteinase K (PK) Solution DFS Item

Proteinase K, produced by the fungus Tritirachium album Limber, is a serine protease that exhibits broad cleavage activity. Proteinase K (PK) Solution is supplied at a concentration of 20mg/ml in 10mM Tris-HCl (pH 7.5), 1mM calcium chloride, 50% glycerol.

Promega T4 DNA Ligase DFS Item

For ligation of DNA ends.

Promega Sequencing Grade Modified Trypsin, Frozen DFS Item

Sequencing Grade Trypsin has been manufactured to provide maximum specificity. Lysine residues in porcine trypsin have been modified by reductive methylation, yielding a highly active and stable molecule that is extremely resistant to autolytic digestion.

Invitrogen™ TURBO™ DNase (2 U/µL) DFS Item

Efficiently degrades and digests DNA

Promega Sequencing Grade Modified Trypsin, Lyophilized DFS Item

Sequencing Grade Trypsin has been manufactured to provide maximum specificity. Lysine residues in porcine trypsin have been modified by reductive methylation, yielding a highly active and stable molecule that is extremely resistant to autolytic digestion.

MP Biomedicals™ Proteinase K

Digests native proteins, thereby inactivating enzymes without a denaturation process. MP Biomedicals Proteinase K is a serine endopeptidase that is not affected by calcium ions. It is useful in the isolation of highly native, undamaged DNAs or RNAs.

MP Biomedicals™ Alpha-Amylase

From Bacillus subtilis

Promega Protein Deglycosylation Mix DFS Item

Removes Glycans from both O-Linked and N-Linked glycosylation sites. Protein Deglycosylation Mix contains five protein deglycosidases, with fetuin provided as a deglycosylation substrate control.

Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA) Promotion

Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA).

Thermo Scientific™ FastDigest NotI

The FastDigest NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: CciNI).

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