Enzymes and Inhibitors

Thermo Scientific™ Bovine Serum Albumin (BSA)

Stabilize enzymes, increase PCR yields, and prevent adhesion of enzymes with Thermo Scientific™ Bovine Serum Albumin.

Thermo Scientific™ DNase I, RNase-free (1 U/µL)

Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.

Thermo Scientific™ RNase A, DNase and protease-free (10 mg/mL)

Specifically degrade single-stranded RNA at C and U residues with the endoribonuclease RNase A.

Thermo Scientific™ T4 DNA Ligase (5 U/µL)

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

Thermo Scientific™ FastDigest BamHI

Cut at G^GATCC sites with Thermo Scientific™ FastDigest™ BamHI restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Invitrogen™ TURBO DNA-free™ Kit DFS Item

Ideal tool for clearing residual DNA

Invitrogen™ DNase I, Amplification Grade DFS Item

Purified and tested for nondetectable levels of RNase contamination

Promega Trypsin Gold, Mass Spectrometry Grade DFS Item

Trypsin Gold, Mass Spectrometry Grade, provides maximum specificity for mass spectrometry.

Thermo Scientific™ FastDigest DpnI

Cut at Gm6A^TC sites with Thermo Scientific™ FastDigest™ DpnI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: MalI).

Thermo Scientific™ RiboLock RNase Inhibitor (40 U/µL)

RiboLock RNase Inhibitor specifically inhibits the activity of RNases A, B, and C, protecting RNA under a variety of reaction conditions.

Promega RQ1 RNase-Free DNase DFS Item

RQ1 (RNA Qualified) RNase-Free DNase is a DNase I that degrades both double-stranded and signle-stranded DNA.

Invitrogen™ Ambion™ DNase I (RNase-free) DFS Item

Nonspecific endonuclease that degrades double- and single-stranded DNA and chromatin

Thermo Scientific™ FastDigest EcoRI

The FastDigest EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Thermo Scientific™ AarI (2 U/µL)

Cut at CACCTGC(4/8)^ sites with AarI restriction enzyme, which performs best at 37°C in its own unique (+oligo) buffer.

Invitrogen™ DNA-free™ DNA Removal Kit DFS Item

Safely eliminate DNA contamination from RNA samples

Thermo Scientific™ Buffer R (10X)

Ensure the optimum reaction conditions for restriction enzymes with Thermo Scientific™ 10X Buffer R, premixed with BSA for enhanced stability.

Thermo Scientific™ TAE Buffer (Tris-acetate-EDTA) (50X)

Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA).

Thermo Scientific™ Tango Buffer (10X)

Ensure the optimum reaction conditions for restriction enzymes with Thermo Scientific™ 10X Buffer Tango, premixed with BSA for enhanced stability.

Thermo Scientific™ FastAP Thermosensitive Alkaline Phosphatase (1 U/µL)

Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase .

Promega Proteinase K DFS Item

Serine protease that exhibits a very broad cleavage specificity. Useful in many molecular biology applications.

Promega Protein Deglycosylation Mix DFS Item

Removes Glycans from both O-Linked and N-Linked glycosylation sites. Protein Deglycosylation Mix contains five protein deglycosidases, with fetuin provided as a deglycosylation substrate control.

Thermo Scientific™ FastDigest Esp3I (IIs class)

Cut at CGTCTC(1/5)^ sites with Thermo Scientific™ FastDigest™ BsmBI (Esp3I), which peforms best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Thermo Scientific™ FastDigest HindIII

The FastDigest HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Invitrogen™ UltraPure™ Glycogen DFS Item

Used as carrier for nucleic acid precipitation in ethanol

Thermo Scientific™ TBE Buffer (Tris-borate-EDTA) (10X)

Perform electrophoresis of nucleic acids in agarose and polyacrylamide gels using Thermo Scientific™ 10X TBE Buffer (Tris-borate-EDTA).

Thermo Scientific™ RNase T1 (1000 U/µL)

RNase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues.

Promega Sequencing Grade Modified Trypsin, Lyophilized DFS Item

Sequencing Grade Trypsin has been manufactured to provide maximum specificity. Lysine residues in porcine trypsin have been modified by reductive methylation, yielding a highly active and stable molecule that is extremely resistant to autolytic digestion.

Thermo Scientific™ Proteinase K, recombinant, PCR grade

Degrade proteins even in the presence of detergents with Proteinase K, a broad-range endolytic protease widely used for digestion of proteins in nucleic acid preparations.

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