Biological Buffers

Gibco™ HEPES (1M) DFS Item

A zwitterionic organic chemical buffering agent commonly used in cell culture media

Thermo Scientific™ DNA Gel Loading Dye (6X)

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.

Thermo Scientific™ Blocker™ Casein

Block membranes, plates and other samples with these ready-to-use PBS or TBS solutions of purified casein for Western blot, ELISA and other detection methods.

HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCO)CCS(=O)(=O)O

Invitrogen™ GlycoBlue™ Coprecipitant (15 mg/mL) DFS Item

Blue dye increases pellet visibility

MES (Fine White Crystals), Fisher BioReagents

CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.248 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N Synonym: 2-(4-Morpholino)ethane Sulfonic Acid, 2-(N-Morpholino)ethanesulfonic acid hydrate PubChem CID: 16218417 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid;hydrate SMILES: C1COCCN1CCS(=O)(=O)O.O

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.

Promega Blue/Orange Loading Dye, 6X DFS Item

Convenient marker dye used for loading DNA samples into gel electrophoresis wells and tracking migration during electrophoresis.

Invitrogen™ UltraPure™ Glycerol DFS Item

Low temperature enzyme storage and concentration

Invitrogen™ EDTA (0.5 M), pH 8.0, RNase-free DFS Item

Ready-to-use, certified RNase-free

Invitrogen™ NuPAGE™ Transfer Buffer (20X) DFS Item

Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting

Thermo Scientific™ Blocker™ BSA (10X) in PBS

Block membranes, plates and other samples with these ready-to-use 10X PBS or TBS solutions of bovine serum albumin for Western blot, ELISA and other detection methods.

Invitrogen™ LiCl Precipitation Solution (7.5 M) DFS Item

Will not precipitate DNA, protein, or carbohydrates

Invitrogen™ Bolt™ Antioxidant DFS Item

A proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis

Invitrogen™ Proteinase K Solution (20 mg/mL), RNA grade DFS Item

Remains active under various conditions

Thermo Scientific™ SuperBlock™ (PBS) Blocking Buffer

Block membranes, plates and tissue samples with these optimized solutions containing a protein that provides fast and effective blocking for Western blot, ELISA and IHC.

Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.

Invitrogen™ NaCl (5 M), RNase-free DFS Item

Ready-to-use, certified RNase-free

Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X) DFS Item

Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels

Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (20X) DFS Item

Made with high-purity reagents and are strictly quality controlled

Invitrogen™ NativePAGE™ Running Buffer (20X) DFS Item

Used to make the NativePAGE Cathode and Anode Running Buffers

Alfa Aesar™ Laemmli SDS Sample Buffer, reducing, 6X

For protein sample preparation to be used in the Laemmli SDS-PAGE system

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