Western Blot Products
Western Blot Products
Kits, buffers, blocking agents, reagents, membranes and substrates for use in conducting western blotting and ELISA immunoassays; includes accessories, modules, and replacement parts and solutions. Steps include: sample preparation, gel electrophoresis, membrane transfer, antibody probe, detection, imaging, and analysis.
The western blot (WB) is a common protein detection and analysis method. A protein sample is denatured to prepare it for gel electrophoresis. Following separation by electrophoresis, and then transferred (blotted) from the gel to a membrane for specific visualization. The blot is covered with a buffer that contains an antibody that will bind to specific parts of the protein being studied. A secondary antibody (an antibody to the primary antibody) is then added (to bind to the primary antibody). The secondary antibody is then visualized by staining, immunofluorescence, radioactivity, or other methods.
Search our extensive portfolio of primary antibodies and secondary antibodies specifically designed for western blotting.
MilliporeSigma™ Calbiochem™ MOWIOL™ 4-88 Reagent
Used for mounting cells on slides for detection of cell-surface antigens.
MilliporeSigma™ Novagen™, Biotinylated Thrombin
Removes enzyme from cleavage reactions using immobilized streptavidin.
MilliporeSigma™ Novagen™ Strep·Tag™ II Antibody HRP Conjugate
Peroxidase-conjugated Strep·Tag II monoclonal antibody useful as single detection reagent, eliminating cross-reactivity associated with secondary reagents
MilliporeSigma™ Novagen™ Strep·Tag™ II Monoclonal Antibody
Mouse monoclonal antibody (IgG1) that has high specificity and affinity for 8-aa Strep·Tag II peptide
MilliporeSigma™ Calbiochem™ PBS-TWEEN™ Tablets
Dissolve one tablet in 1 liter of deionized water to yield 140 mM NaCl, 3 mM KCl, 0.05% TWEEN™ 20 Detergent, 10 mM phosphate buffer, pH 7.4 at 25°C
MilliporeSigma™ Calbiochem™ Protein G, Peroxidase Conjugate
Purified recombinant protein G conjugated to peroxidase
MilliporeSigma™ Calbiochem™ SeaBlock™ Reagent, Salmon Plasma
Ready to use blocking reagent for western blotting immunoassays.
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MilliporeSigma™ ReBlot Plus Kit and Reagents
Blots can be stripped of antibodies in approximately 15 minutes at room temperature
MilliporeSigma™ Chemicon™ Choline Acetyltransferase Blocking Peptide
For Blocking AB143, AB144P Antibodies
MilliporeSigma™ Immobilon™ Blotting Filter Paper
Chromatography-grade blotting filter paper precut to the most popular Western blotting sizes
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MilliporeSigma™ Immobilon™-FL PVDF Membranes
Designed specifically for Western blots and protein sequencing. Immobilon™-FL PVDF Transfer Membranes are plain surfaced with 0.45 μm pores and have lower background fluorescence than other membranes. Available in 10 and 20 cm squares and a 3.75 m roll.
MilliporeSigma™ Immobilon™ GO for Simple Immunodetection
Immobilon™ GO applies the principles of lateral flow assays for walk away processing of Western blots. Simply place your miniblot in the device and then add your wash and antibody solutions to the ports in the top. Return later to apply detection substrate and image your Western blot.
MilliporeSigma™ Immobilon-Ny+ Membrane, Charged Nylon, 0.45 μm
For nucleic acid blotting and screening
MilliporeSigma™ Immobilon™-P Blotting Sandwiches
Immobilon-P membrane interleaved with blotting paper, for standard tank transfer procedures.
MilliporeSigma™ Immobilon™-P PVDF Membrane
Hydrophobic PVDF Transfer Membrane for western blotting
MilliporeSigma™ Immobilon-P PVDF Membranes, 0.45 μm
Immobilon Transfer Membranes offer the convenience of pre-cut sheets with the flexibility of membrane rolls
MilliporeSigma™ Immobilon™-PSQ PVDF Membranes
For Western blots and protein sequencing
MilliporeSigma™ Immobilon-PSQ PVDF Membranes
Immobilon Transfer Membranes offer the convenience of pre-cut sheets with the flexibility of membrane rolls
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MilliporeSigma™ Immobilon-E Blotting Sandwich
MilliporeSigma™ Immobilion™-E Blotting Sandwiches are a convenient way to process multiple Western Blots
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MilliporeSigma™ Immobilon NOW Dispenser
Immobilon Transfer Membranes offer the convenience of pre-cut sheets with the flexibility of membrane rolls
MilliporeSigma™ Immobilon™-E Transfer Membrane
Immobilon™-E provides the a PVDF blotting membrane that wets in transfer buffer or water and eliminates the need for the alcohol pre-wet step.
MilliporeSigma™ Luminata Crescendo ELISA HRP Substrate
Used on immunoblots as chemiluminescent substrate
MilliporeSigma™ Calbiochem™ DNP-Albumin Conjugate, Bovine
For production of antibodies specific for DNP and albumin
MilliporeSigma™ Calbiochem™ SignalBoost™ Immunoreaction Enhancer Kit
Designed to enhance desired signal in assays such as immunoblotting, dot blotting, ELISA.
MilliporeSigma™ Calbiochem™ ImmunoPen™
Useful in immunostaining techniques on paraffin, frozen sections cytology preparations.
MilliporeSigma™ HAT Assay Buffer, 5X
For use in the HAT Assay.
MilliporeSigma™ NOW Bundle, Immobilon-P
Bundle includes the Immobilon NOW Dispenser and two 8.5cm x 10m rolls of Immobilon-P transfer membrane
MilliporeSigma™ SNAP i.d.™ 2.0 Protein Detection System: Antibody Collection Tray
Antibody collection tray for SNAP i.d. 2.0 Protein Detection Systems
MilliporeSigma™ SNAP i.d.™ 2.0 Protein Detection System: Blot Roller
Blot roller for SNAP i.d. 2.0 Protein Detection Systems
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