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Thermo Scientific™ MuSeek™ Library Preparation Kit for Ion Torrent™

Produce Ion Torrent™-compatible next-gen sequencing libraries from as little as 100 ng starting DNA. DNA fragmentation and transposon-tagging are accomplished in one step.

Manufacturer:  Thermo Scientific™ K1331

Catalog No. FERK1331



Description

Description

Thermo Scientific MuSeek Library Preparation Kit for Ion Torrent™ is designed for generation of high–quality genomic DNA libraries for sequencing with the Ion Personal Genome Machine™ (PGM™) and Ion Proton™ systems. The fast protocol utilizes MuA transposase enzyme, which catalyzes simultaneous fragmentation of double-stranded target DNA and tagging the fragment ends with transposon DNA. In a subsequent PCR step the platform-specific adaptors are added using a robust and accurate Thermo Scientific Phusion™ High-Fidelity DNA polymerase. Starting with only 100 ng of the sample DNA the same protocol can be used to generate 100-400 bp insert libraries.
Highlights

  • Fast - generates NGS fragment library in less than 80 minutes
  • Convenient - no physical shearing, end-repair and ligation steps
  • Easy to use - same protocol for generating different length DNA fragment libraries
Applications
DNA fragment library preparation for downstream use in next generation sequencing workflow.

Includes
  • MuSeek Enzyme Mix
  • MuSeek Fragmentation Reaction Buffer
  • MuSeek Stop Solution
  • Control DNA
  • MuSeek Adaptor Addition Primer Mix
  • MuSeek Adaptor Addition Reaction Buffer
  • Phusion Hot Start II High-Fidelity DNA Polymerase
  • MuSeek Sequencing Primer
    Note: The kit is shipped on dry ice. Upon arrival, open the kit and place MuSeek Enzyme Mix for storage below -70°C, avoid repeated freeze-thaw cycles. The Stop Solution can be stored at room temperature, and the remaining kit components at -20°C.

Hazardous: No
Shelf Life:12 Months
Shipping Condition: Dry Ice

Specifications

Specifications

MuSeek Enzyme Mix, MuSeek Fragmentation Reaction Buffer, MuSeek Stop Solution, Control DNA, MuSeek Adaptor Addition Primer Mix, MuSeek Adaptor Addition Reaction Buffer, Phusion Hot Start II High-Fidelity DNA Polymerase, MuSeek Sequencing Primer
DNA fragment library preparation for downstream use in next generation sequencing workflow.
10 reactions
The kit is shipped on dry ice. Upon arrival, open the kit and place MuSeek Enzyme Mix for storage below -70deg.C, avoid repeated freeze-thaw cycles. The Stop Solution can be stored at room temperature, and the remaining kit components at -20deg.C.
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