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Thermo Scientific™ MuSeek™ Barcode Set 1, Ion Torrent™ Compatible

Produce Ion Torrent™-compatible next-gen sequencing libraries from as little as 100 ng starting DNA. DNA fragmentation and transposon-tagging are accomplished in one step.

Manufacturer:  Thermo Scientific™ K1541

Catalog No. FERK1541



Description

Description

Thermo Scientific MuSeek Library Preparation Kit for Ion Torrent™ is designed for generation of high-quality genomic DNA libraries for sequencing with the Ion Personal Genome Machine™ (PGM™) and Ion Proton™ systems. The fast protocol utilizes MuA transposase enzyme, which catalyzes simultaneous fragmentation of double-stranded target DNA and tagging the fragment ends with transposon DNA. In a subsequent PCR step the platform-specific adaptors are added using a robust and accurate Thermo Scientific Phusion™ High-Fidelity DNA polymerase. Starting with only 100 ng of the sample DNA the same protocol can be used to generate 100-400 bp insert libraries.

Highlights

  • Fast - generates NGS fragment library in less than 80 minutes
  • Convenient - no physical shearing, end-repair and ligation steps
  • Easy to use - same protocol for generating different length DNA fragment libraries
Applications
DNA fragment library preparation for downstream use in next generation sequencing workflow.

Includes
  • MuSeek Enzyme Mix
  • MuSeek Fragmentation Reaction Buffer
  • MuSeek Stop Solution
  • Control DNA
  • MuSeek Adaptor Addition Primer Mix
  • MuSeek Adaptor Addition Reaction Buffer
  • Phusion Hot Start II High-Fidelity DNA Polymerase
  • MuSeek Sequencing Primer
Note: The kit is shipped on dry ice. Upon arrival, open the kit and place MuSeek Enzyme Mix for storage below -70°C, avoid repeated freeze-thaw cycles. The Stop Solution can be stored at room temperature, and the remaining kit components at -20°C.

Specifications

Specifications

MuSeek Barcode Adapter Primer Mix 1 to 16
Barcoded DNA fragment library preparation for downstream use in next generation sequencing workflow.
80 reactions (5 reactions each)
Store at -20deg.C before opening.
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