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Thermo Scientific™ EpiJET™ 5-hmC Enrichment Kit

Manufacturer:  Thermo Scientific™ K1491BID

Catalog No. FERK1491


 

Specifications

Specifications

Enriched DNA can be used for: qPCR analysis of desired loci, microarray analysis or next generation sequencing
Box 1: 5-hmC Modifying Enzyme (250μL), 4X Enzyme Reaction Buffer (350μL), 10x Biotin Conjugation Buffer (250μL), Biotin Reagent 1.25mL, 5μM unmodified Primer Mix for Control DNA (100μL), 5μM Primer Mix for 5-hmC Control DNA (100μL), 5-hmC Control DNA, 10ng/μL (10μL), unmodified Control DNA, 0.5μg/μL Box 2: Streptavidin Magnetic Beads (500μL), Streptavidin Binding Buffer (1mL), Streptavidin Wash Buffer 1 (10mL), Streptavidin Wash Buffer 2 (10mL), Water, nuclease-free (15mL), Column Binding Buffer (5.5mL), concentrated Column Wash Buffer (2 x 7mL), Elution Buffer (2 x 1.5mL), DNA Purification Micro Columns and Collection Tubes (50)
25 reactions
Store all components of Box 1 at -20deg.C. Upon kit arrival remove Streptavidin Magnetic Beads from Box 2 and store at 4deg.C. Store all other components of Box 2 at room temperature (15deg. To 25deg.C).
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