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Promega™ Sequencing Grade Modified Trypsin, Lyophilized DFS Item

Retains at least two times as much activity as unmodified trypsin after a three-hour incubation at 37°C

Manufacturer:  Promega™ V5111

Catalog No. PR-V5111



Includes: 1 × 1mL Trypsin Resuspension Dilution Buffer, 5 × 20μg Sequencing Grade Modified Trypsin, Porcine

Description

Description

Modified by reductive methylation, rendering it extremely resistant to autolytic digestion. Further improved by TPCK treatment followed by affinity purification yielding a highly active and stable molecule.

  • Provided in convenient 20μg aliquots with a stability-optimized resuspension buffer
  • Recommended dilution: protease:protein ratio of 1:100 to 1:20 (w/w)
  • Recommended reaction buffer: 50mM NH4HCO3 (pH 7.8)

Specifications

Specifications

Trypsin
0.1mg/mL
7.7
'Hydrolyzing the Peptide at the Carboxyl side of Lysine and Arginine residues
Lyophilized powder at -20°C, reconstituted enzyme at -70°C
Lyophilized Powder
White
5 x 20μg
50mM Acetic Acid Resuspension Buffer
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