Promega™ Sequencing Grade Modified Trypsin, Lyophilized
Retains at least two times as much activity as unmodified trypsin after a three-hour incubation at 37°C
Manufacturer: Promega™ V5111
Modified by reductive methylation, rendering it extremely resistant to autolytic digestion. Further improved by TPCK treatment followed by affinity purification yielding a highly active and stable molecule.
- Provided in convenient 20μg aliquots with a stability-optimized resuspension buffer
- Recommended dilution: protease:protein ratio of 1:100 to 1:20 (w/w)
- Recommended reaction buffer: 50mM NH4HCO3 (pH 7.8)
|'Hydrolyzing the Peptide at the Carboxyl side of Lysine and Arginine residues|
|Lyophilized powder at -20°C, reconstituted enzyme at -70°C|
|5 x 20μg|
|50mM Acetic Acid Resuspension Buffer|
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