missing translation for 'onlineSavingsMsg'
Learn More
Please login to your online account to display your discounted pricing

Promega™ Sequencing Grade Modified Trypsin, Frozen DFS Item

Improved by TPCK treatment followed by affinity purification yielding a highly active and stable molecule

Manufacturer:  Promega™ V5113

Catalog No. PR-V5113



Description

Description

Trypsin specifically hydrolyzes peptide bonds at the carboxylic sides of lysine and arginine residues. Unmodified trypsin is subject to autolysis, generating fragments that can interfere with protein sequencing, HPLC or mass spectrometry analysis of the peptides. In addition, autolysis can result in the generation of pseudotrypsin, which has been shown to exhibit an additional chymotrypsin-like specificity.

  • Modified by reductive methylation, rendering it extremely resistant to autolytic digestion
  • Retains at least two times as much activity as unmodified trypsin after a three-hour incubation at 37°C in functional stability tests Provided with a stability-optimized dilution buffer
  • Format: frozen liquid
  • Recommended dilution: protease:protein ratio of 1:100 to 1:20 (w/w) for protein sequencing
  • Recommended Reaction Buffer: 50mM NH4HCO3 (pH 7.8)

Specifications

Specifications

Trypsin
0.1mg/mL
7.7
'Hydrolyzing the Peptide at the Carboxyl side of Lysine and Arginine residues
-70°C
Frozen Liquid
Colorless
100μg
50mM Acetic Acid Resuspension Buffer
Documents
Promotions

Promotions