Promega™ Sequencing Grade Modified Trypsin, Frozen
Improved by TPCK treatment followed by affinity purification yielding a highly active and stable molecule
Manufacturer: Promega™ V5113
Trypsin specifically hydrolyzes peptide bonds at the carboxylic sides of lysine and arginine residues. Unmodified trypsin is subject to autolysis, generating fragments that can interfere with protein sequencing, HPLC or mass spectrometry analysis of the peptides. In addition, autolysis can result in the generation of pseudotrypsin, which has been shown to exhibit an additional chymotrypsin-like specificity.
- Modified by reductive methylation, rendering it extremely resistant to autolytic digestion
- Retains at least two times as much activity as unmodified trypsin after a three-hour incubation at 37°C in functional stability tests Provided with a stability-optimized dilution buffer
- Format: frozen liquid
- Recommended dilution: protease:protein ratio of 1:100 to 1:20 (w/w) for protein sequencing
- Recommended Reaction Buffer: 50mM NH4HCO3 (pH 7.8)
|'Hydrolyzing the Peptide at the Carboxyl side of Lysine and Arginine residues|
|50mM Acetic Acid Resuspension Buffer|
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