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Promega™ Griess Reagent System DFS Item

Measures nitrite (NO2-), one of two primary stable and nonvolatile breakdown products of nitric oxide (NO)

Manufacturer:  Promega™ G2930

Catalog No. PR-G2930


 

Description

Description

Nitric oxide is an important physiological messenger and effector molecule in many biological systems, including immunological, neuronal and cardiovascular tissues. This assay relies on a diazotization reaction that was originally described by Griess in 1879.

  • Based on a chemical reaction that uses sulfanilamide and N-1-naphthylethylenediamine dihydrochloride (NED) under acidic (phosphoric acid) conditions
  • Detects NO2- in a variety of biological and experimental liquid matrices such as plasma, serum, urine and tissue culture medium
  • Nitrite sensitivity is dependent on the matrix
  • Limit of detection is 2.5μM (125pmol) nitrite (in ultrapure, deionized, distilled water)

Specifications

Specifications

1000 Assays
Plastic Bottle
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