Invitrogen™ Human IFN-alpha A (alpha 2a) Recombinant Protein

Description

Human IFN alpha A (Alpha 2a) Recombinant Protein, 111001, from Invitrogen., 111001, from Invitrogen. Species Reactivity: Human; Applications: ELISA, Immunoprecipitation, Western Blot.

The protein encoded by this gene is produced by macrophages and has antiviral activity. Interferon was titrated with the use of the cytopathic effect inhibition assay [Budd et al. (1985) Canc. Chem. Pharm. 12:39]. The EC50 for interferon in this assay is ∽1 U/ml. The units are determined by use of a Human IFN Alpha A (Hu IFN- alpha A [2a]) laboratory standard calibrated to the international reference standard for Human Interferon Alpha A provided by the National Institutes of Health [Meager et al. (2001) J. Immunol Methods. 257(1-2):17-33]. Store at -70°C or below for retention of full activity. Thaw product vial by incubation in cold tap water until just thawed. Aliquot and refreeze unused contents to minimize effects of freeze thawing. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of product should be in buffers containing protein such as 0.1% BSA or tissue culture media with serum. Dilution for freezing of material below 2 x 10^5 units/ml is not recommended. One freeze thaw cycle is equivalent to thawing an aliquot prepared from the material received.