missing translation for 'onlineSavingsMsg'
Learn More
Please login to your online account to display your discounted pricing

Invitrogen™ Novex™ DYNAL™ Dynabeads™ Antibody Coupling Kit DFS Item

Allows easy coupling of an antibody of your choice to the surface of uniform, 2.8μm superparamagnetic Dynabeads M-270 Epoxy beads

Manufacturer:  Invitrogen™ LS14311D

Catalog No. 14311D



Includes: 60mg Dynabeads M-270 Epoxy and Buffers required for covalent coupling. Dynabeads M-270 Epoxy supplied freeze dried. Multiple buffers provided in various volumes.

Description

Description

Following immobilization of your antibody, the beads can then be used for experiments such as immunoassays, immunoprecipitation (IP), co-immunoprecipitation (co-IP) of protein complexes, co-IP of protein-nucleic acid complexes, as well as many other downstream applications. The Dynabeads Epoxy beads in this kit do not contain Tween; detergent, and so are ideal for use in mass spectrometry (MS) analysis. In addition to Dynabeads, the kit supplies all buffers needed for the coupling and washing steps.

  • Optimized buffers and Epoxy-coated Dynabeads included in kit
  • Easy-to-use protocol with minimal hands-on time
  • Covalent antibody coupling to the Dynabeads avoids co-elution of the antibody with the target protein
  • Final Product: Antibody (or other protein ligand) coupled Dynabeads
  • Sample Type: Intact Proteins

Efficient, easy coupling

  • Antibodies are covalently coupled to Dynabeads M-270 Epoxy beads, minimizing the risk of bound antibody contaminating your final eluate
  • Dynabeads M-270 Epoxy beads exhibit ultra-low background binding and don't require blocking before use
  • Other proteins such as lectins and enzymes may also be coupled using the same beads, buffers, and protocol (depending on the stability and functionality of the protein/ligand you are using)
Magnetic bead-based separation offers easy handling
  • Captured proteins and protein complexes are easily separated, washed and eluted using a DynaMag magnet and magnetic separation properties of Dynabeads
  • Supplied buffers are optimized to give consistent and reliable results and the kit includes five different binding, washing, and elution buffers, in addition to Dynabead M-270 Epoxy

Not high-throughput compatible (Manual)

Immunoprecipitation, Protein Sample Preparation and Protein Purification, Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Room Temperature

Specifications

Specifications

Immunoprecipitation, Protein Sample Preparation and Protein Purification, Proteins, Expression, Isolation and Analysis
60mg Dynabeads M-270 Epoxy and Buffers required for covalent coupling. Dynabeads M-270 Epoxy supplied freeze dried. Multiple buffers provided in various volumes.
1 kit
Intact Proteins
Documents

For Research Use Only. Not for use in diagnostic procedures.