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Thermo Scientific™ North2South™ Biotin Random Prime Labeling Kit

Produce biotinylated DNA templates for DNA hybridization and detection methods using random heptanucleotides, Klenow fragment and biotin-nucleotides.

Manufacturer:  Thermo Scientific™ PI17075

Catalog No. PI17075



Thermo Scientific™ North2South Random Prime DNA Biotinylation Kit uses random heptanucleotides, Klenow fragment and biotin-nucleotides to produce biotinylated DNA templates for DNA hybridization and detection methods.

Random heptanucleotides containing all possible sequences are annealed to a denatured DNA template. These act as primers for complementary strand synthesis by DNA polymerase. Biotinylated dNTPs in the reaction mix are incorporated into the newly synthesized DNA. This protocol yields biotin-labeled DNA of high activity for use as probes in hybridization experiments such as Southern and Northern hybridizations. The resulting hybrids can be detected with streptavidin-horseradish peroxidase (HRP).


  • Ultimate sensitivity - labels with biotinylated dUTP, maximizing streptavidin-HRP binding and increasing sensitivity
  • Works with as little as 100ng starting DNA template
  • Exonuclease-free Klenow fragment produces higher yields
  • Each reaction yields probe sufficient for approximately three (10 x 10cm) blots


Non-radioactive Southern blotting
5X Heptanucleotide Mix, 100µL;5X Deoxynucleotide Mix, 80µL;10X Reaction Buffer, 50µL;Klenow DNA Polymerase, 10µL;Control DNA, 5µL;5 M Ammonium Acetate, 1mL;0.5 M EDTA, pH 8.0, 1mL;Nuclease-free Water, 1mL;Biotin-11-UTP, 20µL
North2South DNA Probe Biotinylation Kit

Multi-component kit with reagents for DNA labeling

10 biotin-labeling reactions (each with 100ng DNA)

10-rxn kit