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Invitrogen™ Molecular Probes™ Rhodamine 110, bis-(CBZ-L-Phenylalanyl-L-Arginine Amide), Dihydrochloride DFS Item

Sensitive and selective substrate for assaying proteases

Manufacturer:  Invitrogen™ LSR6502

Catalog No. R6502



Description

Description

This Molecular Probes™ bisamide derivative of rhodamine 110 (R110) is a sensitive and selective substrate for assaying proteases in solution or inside living cells. These fluorogenic substrates contain an amino acid or peptide covalently linked to each of R110's amino groups. Upon enzymatic cleavage, the nonfluorescent bisamide substrate is converted first to the fluorescent monoamide and then to R110, with a further increase in fluorescence.

  • Substrate can be used to continuously measure enzyme activity in cell extracts and purified enzyme preparations using fluorometer or fluorescence microplate reader
  • Substrate name: (CBZ-Phe-Arg)2-R110
  • Useful to measure: Plasmin, cathepsin L
  • Ex/Em of R110 product: (498/521nm)
  • Cell-Permeant

Properties of the R110 Reaction Product

  • Has spectral properties similar to those of fluorescein, making it compatible with standard fluorescein filter sets
  • Signal to background ratios are good because of large extinction coefficient of R110
  • Unlike fluorescein, fluorescence intensity of rhodamine 110 reaction product is constant from pH 3–9

Enzyme and Protein Activity Assays, Protease/Peptidase Activity, Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Room temperature

Specifications

Specifications

5mg
Fluorescent
Substrate, Proteinase, Rhodamine 110 based
Store in freezer (-5° to -30°C)
Flow Cytometer, Fluorescence Microscope
Cathepsin, Plasmin
Documents

For Research Use Only. Not for human or animal therapeutic or diagnostic use.