Invitrogen™ Molecular Probes™ Fluorocillin™ Green 495/525 -Lactamase Substrate, Soluble Product
Reporter for certain ELISA protocols
Manufacturer: Invitrogen™ LSF33952
Fluorocillin™ Green substrate is a robust reporter for ELISA protocols that employ TEM-1 β-lactamase conjugates. Upon cleavage, Fluorocillin™ Green reagent is converted to a green-fluorescent soluble product (emission maxima at approx. 525nm). Fluorocillin Green reagent has a broad dynamic range of fluorescence signal, is more sensitive than common colorimetric substrates, and displays only modest hydrolysis when incubated for extended periods from pH 5.5 to 8.0. Additionally, Fluorocillin Green reagent consistently reports β-lactamase activity in the presence of EDTA, many detergents, salts, and sodium azide. Fluorocillin Green reagent is a component of the SensiFlex™ ELISA Development Kits.
Beta-Lactamase Activity Assays, Cell Analysis, Cell-Based β-Lactamase Assays, Cell-Based Reporter Assays, Cellular Imaging, Enzyme and Protein Activity Assays, Proteins, Expression, Isolation and Analysis, Reporter Assays
Shipping Condition: Room temperature
|5 x 100μg|
|Store in freezer (-5° to -30°C) and protect from light.|
For Research Use Only. Not for use in diagnostic procedures.