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Invitrogen™ Molecular Probes™ CM-H2DCFDA General Oxidative Stress Indicator DFS Item

General oxidative stress indicator

Manufacturer:  Invitrogen™ LSC6827

Catalog No. C6827


 

Description

Description

CM-H2DCFDA is chloromethyl derivative of H2DCFDA useful as indicator for reactive oxygen species (ROS) in cells. This indicator exhibits much better retention in live cells than H2DCFDA. CM-H2DCFDA passively diffuses into cells, where its acetate groups are cleaved by intracellular esterases and its thiol-reactive chloromethyl group reacts with intracellular glutathione and other thiols. Subsequent oxidation yields a fluorescent adduct that is trapped inside the cell, thus facilitating long-term studies.

  • Ex/Em: approx. 492 - 495/517–527nm
  • Product is air sensitive (store under dry argon or nitrogen)
  • Product may be dissolved in DMSO, DMF, or ethanol for use
  • Indicator is cell permeant
  • Fluorescence can be monitored using a flow cytometer, fluorometer, microplate reader, or fluorescence microscope, using excitation sources and filters appropriate for fluorescein

Refer to the Molecular Probes™ Handbook for additional product information.

Apoptosis, Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Free Radical Detection, Nitro-Oxidative Stress, Reactive Oxygen Species

Order Info

Shipping Condition: Room Temperature

Specifications

Specifications

20 x 50μg
Store in freezer (-5° to -30°C).
Fluorescent Dye-Based
Free Radical Sensor
Documents

For Research Use Only. Not for use in diagnostic procedures.