Invitrogen™ Molecular Probes™ CellEvent™ Caspase-3/7 Green Detection Reagent
Optimized reagent for analysis of apoptotic cells
Manufacturer: Invitrogen™ LSC10423
CellEvent™ Caspase-3/7 Green Detection Reagent is a novel fluorogenic substrate for activated caspase-3/7 that is compatible with both live cell and fixed imaging, with absorption/emission maxima approx. 502/530nm. Activation of caspase-3 is considered an essential event during apoptosis, making this an optimized reagent for analysis of apoptotic cells.
- Optimized caspase-3/7 substrate for apoptosis analysis
- Simple, no-wash protocol helps preserve delicate apoptotic cells
- Compatible with both live cell fluorescence-imaging and formaldehyde-based fixation methods
The CellEvent Caspase-3/7 Green Detection Reagent is a four amino acid peptide (DEVD), conjugated to a nucleic acid binding dye with an absorption/emission maxima of approx. 502/530nm. The DEVD peptide sequence is a cleavage site for caspase-3/7 and the conjugated dye is non-fluorescent until cleaved from the peptide and bound to DNA.
Principles of CellEvent Caspase-3/7 Apoptosis Detection Reagent
The CellEvent Caspase-3/7 Green Detection Reagent is intrinsically non-fluorescent as the DEVD peptide inhibits the ability of the dye to bind to DNA. However, after activation of caspase-3/7 in apoptotic cells, the DEVD peptide is cleaved enabling the dye to bind to DNA and produce a bright, fluorogenic response. The fluorescence emission of the dye when bound to DNA is approx. 530nm and can be observed using a standard FITC filter set.
Simple Three Step Protocol
To use CellEvent Caspase 3/7 Detection Reagent simply add substrate to cells, incubate 30 minutes, and visualize. Apoptotic cells with activated caspase-3/7 will have bright green nuclei, while cells without activated caspase 3/7 will have minimal fluorescence signal .
Assay Versatility Allows for Live Cell Detection and Fixation
Robust assay enables researchers to examine caspase-3/7 activation in live cells. Furthermore, since wash steps are not necessary for detection, fragile apoptotic cells typically lost during wash steps are preserved. Importantly, the fluorescent signal from CellEvent Caspase 3/7 Detection Reagent can survive fixation and permeabilization. This allows for the flexibility to perform end-point assays, and probe for other proteins of interest using immunocytochemistrt.
Apoptosis, Apoptosis Related Factors, Caspase Activation, Cell Analysis, Cell Viability, Proliferation and Function, Cellular Imaging, High-Content Screening (HCS)
Shipping Condition: Room temperature
|100μL, 2mM solution in DMSO.|
|Store at ≤-20°C, dessicate, protect from light|
|Fluorescence Microscope, High Content Instrument|
|CellEvent Caspase 3/7 Green|
For Research Use Only. Not for use in diagnostic procedures.