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Invitrogen™ LR Clonase™ II Plus enzyme DFS Item

Mix contains a proprietary blend of Int (Integrase), IHF (Integration Host Factor), and Xis (Excisionase) enzymes.

Supplier:  Invitrogen™ 12538120

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Catalog No. 12538120


Includes:
  • LR Clonase II Plus enzyme and proteinase K solution (2 μg/μL)

Description

Description

Gateway™ LR Clonase™ enzyme mix contains a proprietary blend of Int (Integrase), IHF (Integration Host Factor) and Xis (Excisionase) enzymes that catalyze the in vitro recombination between an Entry clone (containing a gene of interest flanked by attL sites) and a Destination vector (containing attR sites) to generate your expression clone. We offer different formats of LR Clonase enzyme mixtures, depending on your application and desired format.

  • Gateway™ LR Clonase™ enzyme mix contains a proprietary blend of Int (Integrase), IHF (Integration Host Factor) and Xis (Excisionase) enzymes
  • They catalyze the in vitro recombination between an Entry clone (containing a gene of interest flanked by attL sites) and a Destination vector (containing attR sites) to generate an expression clone
  • Different formats of LR Clonase enzyme mixtures are offered depending on the application and desired format
  • LR Clonase II Plus enzyme mix is the latest version that offers the highest recombination efficiency available
  • It is specifically optimized for both single and multiple fragment cloning
  • LR Clonase II Plus enzyme mix is provided in an optimized single mix of enzyme and reaction buffer
  • This ensures enzyme stability and ease of use with few pippetting steps for both single and multiple fragment cloning
  • Guaranteed stable for 6 months when properly stored at -20°C
Specifications

Specifications

Includes LR Clonase II Plus enzyme and proteinase K solution (2 μg/μL). Store at -20°C. Guaranteed stable for 6 months when properly stored.
LR Clonase
20 Reactions
Clonase™, Gateway™
Dry Ice
Reaction Buffer
LR Clonase Enzyme
SDS
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For Research Use Only. Not for use in diagnostic procedures.