Thermo Scientific™ Imperial™ Protein Stain

Description

Thermo Scientific™ Imperial Protein Stain is a unique, ready-to-use colorimetric stain formulated with coomassie dye R-250 that delivers fast and sensitive total protein staining performance for low nanogram-level detection of proteins in polyacrylamide electrophoresis gels.

Imperial Protein Stain is a coomassie dye reagent for detection of protein bands in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and 2D gels. The stain is a unique formulation of coomassie brilliant blue R-250 that delivers substantial improvements in protein-staining performance compared to homemade or other commercial stains. Staining results in intensely colored protein bands that are easy to photograph and document with gel imagers. This reagent is one of the most sensitive colorimetric stains available, easily detecting 3 to 6 nanograms of protein per band. The Imperial Protein Stain protocol uses simple water-wash steps rather than methanol/acetic acid fixation and destaining, which saves valuable preparation time and minimizes reagent costs.

Highlights:

• Sensitive – detects less than 3ng protein per band with the enhanced protocol (3 hours)

• Fast – ready-to-use reagent detects less than 6ng protein per band in just 20 minutes

• Robust – highly consistent, reproducible protein staining technique

• High contrast – intense purple bands are easier to photograph or scan than typical coomassie blue stains

• Versatile – compatible with downstream mass spectrometry analysis and protein sequencing

• Convenient – water washes only; no acid-fixative or methanol destaining solutions required

• Stable – 1-year room-temperature stability ensures consistent performance and saves refrigerator space

• Flexible – adjust staining and washing times to meet time- and sensitivity-requirements