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Gibco™ B-27™ Plus Supplement (50X) DFS Item

B-27 Plus Supplement is the next-generation of the most cited neuronal cell culture product, B-27 Supplement

Manufacturer:  Life Technologies LSA3582801

Catalog No. A3582801



B-27 Plus Supplement is a serum-free supplement that provides the highest rate of in vitro survival of primary rodent and human stem cell-derived neurons when used with Neurobasal Plus Medium. B-27 Plus Supplement features an optimized formulation, upgraded manufacturing process, and more stringent quality control of raw materials and final product. These improvements enable the highest rate of neuronal cell survival, improved electrophysiological activity, and enhanced functional maturity versus other neuronal cell culture media systems.

B-27 Plus Supplement, when combined with Neurobasal Plus Medium, offers:

  • At least 50% higher survival rate of primary rat neurons compared to classic B-27 Supplement and Neurobasal Medium
  • Highest survival rate of primary rat, mouse, and human stem cell-derived neurons compared to other commercially available serum-free neuronal media systems
  •  Improved electrophysiological activity and maturation—more consistent, stable, and synchronized spontaneous activity in primary rodent neurons after 10 days compared to BrainPhys™ Neuronal Medium
  •  Accelerates neurite outgrowth and increases the number of synaptic-positive puncta

Available as a 50X, 10 mL supplement, B-27 Plus Supplement can seamlessly replace other neuronal cell culture supplements, such as classic B-27 Supplement. The B-27 Plus Neuronal Culture System is also available for purchase, which is comprised of B-27 Plus Supplement and Neurobasal Plus Medium, offering a convenient and valuable all-in-one ordering solution.



Neuronal Cells
Maintenance of primary rat, mouse and human PSC-derived and fetal-derived neurons
Store at −5 to −30°C. Protect from light

For Research Use Only. Not for use in diagnostic procedures.