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Thermo Scientific™ RNase A, DNase and protease-free

Specifically degrade single-stranded RNA at C and U residues with the endoribonuclease RNase A.

Manufacturer:  Thermo Scientific™ EN0531

Catalog No. FEREN0531



Description

Description

RNase A, DNase and protease-free is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues.

It cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide. The resulting 2', 3'-cyclic phosphate is hydrolyzed to the corresponding 3'-nucleoside phosphate (see References 1, 2).

Highlights

  • RNase A is free of DNase activity. It is not necessary to heat it before use.
Specifications

Specifications

10mg/mL
Bovine pancreas
10mg
the most potent inhibitor is a mamalian ribonuclease inhibitor, e.g., RiboLock RNase Inhibitor. Other inhibitors: uridine 2 ft., 3 ft.-cyclic vanadate, 5 ft.-diphosphoadenosine 3 ft.-phosphate and 5 ft.-diphosphoadenosine 2 ft.phosphate (2), SDS, diethyl
13.7kDa monomer
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