Thermo Scientific™ RNase A, DNase and protease-free
Specifically degrade single-stranded RNA at C and U residues with the endoribonuclease RNase A.
Manufacturer: Thermo Scientific™ EN0531
RNase A, DNase and protease-free is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues.
It cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide. The resulting 2', 3'-cyclic phosphate is hydrolyzed to the corresponding 3'-nucleoside phosphate (see References 1, 2).
- RNase A is free of DNase activity. It is not necessary to heat it before use.
|the most potent inhibitor is a mamalian ribonuclease inhibitor, e.g., RiboLock RNase Inhibitor. Other inhibitors: uridine 2 ft., 3 ft.-cyclic vanadate, 5 ft.-diphosphoadenosine 3 ft.-phosphate and 5 ft.-diphosphoadenosine 2 ft.phosphate (2), SDS, diethyl|