Thermo Scientific™ EZ-Link™ Sulfo NHS-LC-LC-Biotin, No-Weigh™ Format

Description

Thermo Scientific™ EZ-Link Sulfo-NHS-LC-LC-Biotin is a long-chain biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

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N -Hydroxysulfosuccinimide (Sulfo-NHS) esters of biotin are the most popular type of biotinylation reagent. NHS-activated biotins react efficiently with primary amine groups (-NH2) in pH 7-9 buffers to form stable amide bonds. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for labeling with NHS-activated biotin reagents. Several different NHS esters of biotin are available, with varying properties and spacer arm lengths. The sulfo-NHS ester reagents are water soluble, enabling reactions to be performed in the absence of organic solvents such as DMSO or DMF.

Highlights:

• Forms a stable amide bond with primary amines
• Sulfonated form is water-soluble