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Invitrogen™ Bolt™ 4-12% Bis-Tris Plus Gels, 12-Well DFS Item

Precast polyacrylamide gels designed for optimal separation of small- to medium-sized proteins under denaturing conditions

Manufacturer:  Invitrogen™ LSNW04122BOX

Catalog No. NW04122BOX



Description

Description

Similar to NuPAGE™ Bis-Tris gels, Bolt Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications. Bolt gels are ideal for Western blot transfer and analysis, and any other techniques where protein integrity is crucial. Also use Bolt Bis-Tris Plus gels to obtain optimal results for day-to-day protein separation needs.
Bolt Bis-Tris Plus gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and 4–12% gradient. The gels can be run using Bolt MES or MOPS SDS running buffer to obtain different separation ranges. Bolt Bis-Tris Plus gels consist of a neutral pH-buffered (pH 6.4) polyacrylamide gel and have an operating pH of about 7.0. Bolt Bis-Tris Plus gels also come in four different well formats: 10, 12, 15, and 17 wells.

  • Optimized run conditions—separate proteins using constant voltage in approximately 35 minutes
  • Better protein integrity—neutral pH sample preparation process preserves proteins
  • Improved protein separation—offer sharper bands, high resolution, longer shelf life, and higher accuracy of results than tris-glycine—based gels
  • Wedge-shaped wells—easier sample loading and up to two times higher sample volume than other 1.0mm-thick gels

Specifications

Specifications

Western blot transfer and analysis, Other techniques where protein integrity is crucial, Day-to-day protein separations
4-12% acrylamide
14 to 260 kd (MOPS buffer), 0.3 to 260 kd (MES buffer)
Up to 45μL/well (30μL recommended)
Bis-Tris Plus Gel
12-well
8cm
4° to 25°C
8cm
Documents

For Research Use Only. Not for use in diagnostic procedures.