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Invitrogen™ TURBO DNA-free Kit DFS Item

Ideal tool for clearing residual DNA

Manufacturer:  Invitrogen™ LSAM1907

Catalog No. AM1907



Includes: TURBO DNase, 10X TURBO DNase Buffer, DNase Inactivation Reagent, Nuclease-free Water

Description

Description

Kit contains reagents for efficient, complete digestion of DNA along with removal of enzyme and divalent cations post-digestion

  • Hyperactive TURBO DNase is catalytically superior enzyme compared to wild type DNase I
  • Removes trace quantities of DNA that can interfere with RT-PCR
  • Reagent included to completely remove DNase without phenol treatment or heating

  • Method for genomic DNA removal prior to RT-PCR
  • TURBO DNase is recombinant, engineered form of DNase I that is efficient than wild type DNase I in digesting away trace amounts of unwanted DNA
  • TURBO DNase binds DNA substrates 6-fold more tightly than traditional DNase I, making enzyme tool of choice for clearing residual DNA that can generate a false positive signal in RT-PCR applications
  • TURBO DNase now includes an enhancer that increases effectiveness by two orders of magnitude
  • Efficient DNase and divalent cation removal without organic extraction or precipitation
  • Conventional DNase treatment of RNA samples prior to RT-PCR typically call for inactivation of the DNase by phenol:CHCl3 extraction or heating followed by precipitation step to concentrate the RNA
  • Phenol:CHCl3 extractions can be cumbersome and time-consuming
  • Heating the sample to inactivate DNase can lead to chemical degradation of RNA by divalent cations present in DNase buffer
  • TURBO DNA-free Kit circumvents problems using novel DNase Inactivation Reagent
  • For removing the TURBO DNase from reaction, Inactivation Reagent also binds and removes divalent cations from TURBO DNase Reaction Buffer

PCR & Real-Time PCR, Real Time PCR (qPCR), Reverse Transcription

Order Info

Shipping Conditions: Dry ice

Specifications

Specifications

TURBO DNase, 10X TURBO DNase Buffer, DNase Inactivation Reagent, Nuclease-free Water
50 reactions
Store components at -20°C (Nuclease-free water may be stored at room temperature)
Documents

For Research Use Only. Not for use in diagnostic procedures.