Électrophorèse, Western blot et ELISA

EMD Millipore™ Immobilon™-P PVDF Transfer Membranes

Optimized for binding proteins transferred from a variety of gel matrices. EMD Millipore Immobilon™-P PVDF Transfer Membranes have superior handling, staining, and resistance than nitrocellulose. For use with Western blots and protein sequencing. Available in sheets or rolls.

Thermo Scientific™ PageRuler™ Prestained 10-180kDa Protein Ladder

Mixture of 10 blue-, orange- and green-stained proteins (10 to 180kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

GE Healthcare Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity.  GE Healthcare Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

Agarose (Low-EEO/Multi-Purpose/Molecular Biology Grade), Fisher BioReagents

Chemical Name or Material: Agarose Name Note: Low-EEO/Multi-Purpose CAS: 9012-36-6 Purity Grade: Molecular Biology Grade Purity Grade Notes: DNase- and RNase-Free Physical Form: Powder/Solid Molecular Formula: C12H18O9

Thermo Scientific™ PageRuler™ Plus Prestained 10-250kDa Protein Ladder

Mixture of 9 blue-, orange- and green-stained proteins (10 to 250kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Acrylamide: Bis-Acrylamide 37.5:1 (40% Solution/Electrophoresis), Fisher BioReagents

Chemical Name or Material: Acrylamide: Bis-Acrylamide 37.5:1 Name Note: 40% Solution CAS: 79-06-1 CAS: 110-26-9 CAS: 7732-18-5 Purity Grade: Electrophoresis Grade Purity Grade Notes: DNase-, RNase- and Protease-Free Physical Form: Liquid

Acrylamide: Bis-Acrylamide 29:1 (40% Solution/Electrophoresis), Fisher BioReagents

Chemical Name or Material: Acrylamide: Bis-Acrylamide 29:1 Name Note: 40% Solution CAS: 79-06-1 CAS: 110-26-9 CAS: 7732-18-5 Purity Grade: Electrophoresis Grade Purity Grade Notes: DNase-, RNase- and Protease-Free Physical Form: Liquid

GE Healthcare Amersham™ ECL™ Western Blotting Detection Reagents

Use for all routine Western blotting. GE Healthcare Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world’s first commercially available chemiluminescent detection reagents and still one of the most widely used.

Thermo Scientific™ 6X DNA Loading Dye

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Invitrogen™ Novex™ NuPAGE™ 4-12% Bis-Tris Protein Gels, 1.0mm, 10 well DFS Item

Pre-cast polyacrylamide gels designed to give optimal separation of small- to medium-sized proteins under denaturing conditions

EMD Millipore™ Immobilon™ Western Chemiluminescent HRP Substrate (ECL)

Provides high sensitivity over a broad detection range at an economical price

Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

An enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by western blot analysis

Thermo Scientific™ Restore™ Plus Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.

GE Healthcare Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls

Use for the best results with fluorescence Western blotting detection applications. GE Healthcare Life Sciences™ Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

Thermo Scientific™ Pierce™ Reagent Reservoirs

Rapidly dispense reagents with multi-channel pipettors using these sterile, sturdy and disposable reservoirs molded from high-impact polystyrene.

EMD Millipore™ Luminata™ Western HRP Chemiluminescence Substrates

Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns

Agarose (Broad Separation Range for DNA/RNA/Genetic Analysis Grade), Fisher BioReagents

Chemical Name or Material: Agarose Name Note: Broad Separation Range for DNA/RNA (500bp to 25kb) CAS: 9012-36-6 Purity Grade: Genetic Analysis-Grade Purity Grade Notes: DNase- and RNase-Free Physical Form: Powder/Solid Molecular Formula: C12H18O9 Formula Weight: 306.12

Thermo Scientific™ Spectra™ Multicolor Broad Range Protein Ladder

A 4-color protein standard containing 10 prestained proteins (10 to 260kDa) for use as size standards in gel electrophoresis and Western blotting.

Thermo Scientific™ Pierce™ Silver Stain Kit

Stain proteins in 1D and 2D gels with this rapid, ultra-sensitive and versatile silver stain system that yields consistent and reliable results.

Fisher BioReagents EZ-Run™ Prestained Rec Protein Ladder

Comprised of 10 recombinant proteins covalently coupled to a blue chromophore plus 10 and 72kDa reference bands tagged with green and orange dyes, respectively

GE Healthcare Amersham™ Hyperfilm™ ECL

Use with all blue and green light chemiluminescent systems. GE Healthcare Amersham™ Hyperfilm™ ECL is constructed from a clear base coated on both sides with a photographic emulsion that is protected by an anti-scratch layer.

Invitrogen™ Bolt™ 4-12% Bis-Tris Plus Gels, 10-Well DFS Item

Precast polyacrylamide gels designed for optimal separation of small- to medium-sized proteins under denaturing conditions

Thermo Scientific™ SuperSignal™ West Femto Chemiluminescent Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

Thermo Scientific™ Microplate Sealing Tape

Clear, self-sticking sealing tape for standard microplates.

Invitrogen™ Bolt™ 4-12% Bis-Tris Plus Gels, 12-Well DFS Item

Precast polyacrylamide gels designed for optimal separation of small- to medium-sized proteins under denaturing conditions

Acrylamide (White, Needle-like Crystals/Electrophoresis), Fisher BioReagents

Chemical Name or Material: Acrylamide CAS: 79-06-1 Purity Grade: Electrophoresis Grade Purity Grade Notes: DNase-, RNase- and Protease-Free Physical Form: Solid Assay: ≥99.9% Assay Percent Range: ≥99.9 % Molecular Formula: C3H5NO Linear Formula: CH2:CHCONH2 Formula Weight: 71.08

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