Crosslinking, Labeling and Protein Modification

Invitrogen™ eBioscience™ Propidium Iodide Staining Solution

Ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis. Propidium Iodide binds to double-stranded DNA by intercalating between basepairs, but is excluded from cells with intact plasma membranes.

Invitrogen™ eBioscience™ 7-AAD Viability Staining Solution

Ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis. Can be used in combination with PE- and FITC-conjugated antibodies in 2-color analysis. The advantage of 7-AAD over PI is that there is minimal spectral overlap between these emissions.

Thermo Scientific™ Pierce™ DMP Crosslinker

Crosslink amines to amines with dimethyl pimelimidate (CAS 58537-94-3); composed of imidoester groups and a membrane-permeable, 7-atom spacer arm.

Thermo Scientific™ Pierce™ NHS Crosslinker

Prepare amine-reactive NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysuccinimide, CAS 6066-82-6.

Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-LC-Biotin

Biotinylate protein amines (e.g., lysines) with this doubly long (30.5A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.

Thermo Scientific™ Bond-Breaker™ Neutral pH TCEP Solution

Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.

Thermo Scientific™ Pierce™ 8M Guanidine·HCl Solution

Ready-to-use, 8M solution of guanidinium hydrochloride (GdnCl, GuHCl), CAS 50-01-1; dilute and pH-adjust to any concentration for protein biology methods.

Thermo Scientific™ Pierce™ Immobilized TCEP Disulfide Reducing Gel

Efficiently reduce protein or peptide disulfide bonds with this stable, odorless, beaded agarose resin on which Tris (2-carboxyethyl) phosphine is immobilized.

EMD Millipore™ Calbiochem™ SPUTOLYSIN™ Reagent

Intended for isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum

Thermo Scientific™ Pierce™ EDC Crosslinker

Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.

GE Healthcare Amersham™ Streptavidin-Fluor Conjugates

Use for in situ hybridization and flow cytometry. GE Healthcare Life Sciences™ Amersham™ Streptavidin-Fluor Conjugates consist of streptavidin conjugated to a range of fluorescent dyes.

Thermo Scientific™ Pierce™ In-Solution Tryptic Digestion and Guanidination Kit

Alkylate and digest proteins and then guanidinate the peptide fragments for mass spectrometry (MS) using proteomics-grade trypsin, buffers and reagents.

Thermo Scientific™ Pierce™ DSP Crosslinker

Crosslink amines to amines with dithiobis succinimidyl propionate, composed of NHS-ester groups and disulfide-containing, 12.0A spacer arm and cleavable with reducing agents.

EMD Millipore™ Proteinase K, Lyophilized

Highly active serine protease that exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA

EMD Millipore™ Calbiochem™ Carboxypeptidase B, Porcine Pancreas

Native carboxypeptidase B catalyzes the release of C-terminal from basic amino acids, arginine, lysine, or ornithine from polypeptides

Thermo Scientific™ Pierce™ DSG Crosslinker

Crosslink amines to amines with disuccinimidyl glutarate with NHS-ester groups at the ends of a 7.7A spacer arm; membrane-permeable for intracellular protein crosslinking.

Thermo Scientific™ Pierce™ 2-Mercaptoethylamine·HCl

Pure, crystalline 2-aminoethanethiol (2-MEA-HCl, also called cysteamine-HCl), CAS 156-57-0; selectively reduces antibody hinge-region disulfide bonds.

Thermo Scientific™ Pierce™ LC-SMCC Crosslinker

Crosslink amines to sulfhydryls with this long crosslinker composed of NHS-ester and maleimide groups at ends of a stabilizing cyclohexane-containing, 15-atom spacer arm.

Thermo Scientific Pierce™ EDC, No-Weigh™ Format Crosslinker

Thermo Scientific™ Pierce™ EDC is carboxyl- and amine-reactive zero-length crosslinker. EDC reacts with carboxyl group first and forms amine-reactive O-acylisourea intermediate that quickly reacts with amino group to form amide bond with release of isourea by-product.

Thermo Scientific™ Pierce™ Guanidine·HCl

Pure, crystalline guanidium hydrochloride (GdnHCl, GuHCl), CAS 50-01-1; denaturant for protein biology methods (elution, solubilization, refolding, storage).

Thermo Scientific™ Pierce™ EGS Crosslinker

Crosslink amines to amines with this membrane permeable crosslinker composed of NHS-ester groups and a 16.1A hydroxylamine-cleavable spacer arm.

Thermo Scientific™ Pierce™ Sulfo-MBS Crosslinker

Crosslink amines to sulfhydryls with this short, water-soluble crosslinker composed of sulfo-NHS-ester and maleimide groups at ends of an aromatic, 6-atom (7.3A) spacer arm.

Thermo Scientific™ Pierce™ Traut's Reagent

2-Iminothiolane-HCl (CAS 4781-83-3); an amine-reactive thiolation reagent to cap protein primary amines with sulfhydryl groups for immediate crosslinking.

Thermo Scientific™ Pierce™ Cysteine·HCl

Make sulfhydryl assay standards or perform protein refolding experiments using this pure, crystalline cysteine hydrochloride (Cys-HCl) CAS 52-89-1.

Thermo Scientific™ EZ-Link™ BMCC-Biotin

Biotinylate reduced cysteines (-SH groups) with a maleimide-activated, sulfhydryl-reactive biotinylation reagent containing a stabilizing cyclohexane group in the spacer arm.

Molecular Probes™ Fluorescein Cadaverine; 5-((5-Aminopentyl)thioureidyl)Fluorescein, Dihydrobromide Salt DFS Item

5-((5-aminopentyl)thioureidyl)fluorescein, dihydrobromide salt (fluorescein cadaverine)

Thermo Scientific™ Pierce™ BM(PEG)2 Crosslinker

Crosslink sulfhydryls to sulfhydryls (cysteine residues) with this long, pegylated, water-soluble crosslinker composed of maleimide groups and 2-unit (14.7A) PEG spacer arm.

Thermo Scientific™ Pierce™ Proteases for Mass Spectrometry

Improve sequence coverage and protein identification for mass spectrometry using these individual, highly specific proteases in custom combinations.

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