Crosslinking, Labeling and Protein Modification

Invitrogen™ eBioscience™ 7-AAD Viability Staining Solution

Ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis. Can be used in combination with PE- and FITC-conjugated antibodies in 2-color analysis. The advantage of 7-AAD over PI is that there is minimal spectral overlap between these emissions.

Thermo Scientific™ EZ-Link™ Sulfo-NHS-SS-Biotin and Labeling Kits

Biotinylate protein amines with this cleavable, mid-length (24.3A), water-soluble, NHS-ester biotinylation reagent containing a cleavable disulfide bond in the spacer arm.

Thermo Scientific™ Bond-Breaker™ Neutral pH TCEP Solution

Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.

Thermo Scientific™ Pierce™ DMP Crosslinker

Crosslink amines to amines with dimethyl pimelimidate (CAS 58537-94-3); composed of imidoester groups and a membrane-permeable, 7-atom spacer arm.

Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotin and Labeling Kits

Biotinylate protein amines (e.g., lysines) with our shortest (13.5A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.

Thermo Scientific™ Pierce™ EGS Crosslinker

Crosslink amines to amines with this membrane permeable crosslinker composed of NHS-ester groups and a 16.1A hydroxylamine-cleavable spacer arm.

Thermo Scientific™ Pierce™ 8M Guanidine·HCl Solution

Ready-to-use, 8M solution of guanidinium hydrochloride (GdnCl, GuHCl), CAS 50-01-1; dilute and pH-adjust to any concentration for protein biology methods.

Thermo Scientific™ EZ-Link™ NHS-PEG4-Biotin and Labeling Kits

Biotinylate protein amines and maximize solubility of proteins with this long (29.0A) NHS-ester biotinylation reagent containing a pegylated, water-soluble spacer arm.

Invitrogen™ eBioscience™ Propidium Iodide Staining Solution

Ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis. Propidium Iodide binds to double-stranded DNA by intercalating between basepairs, but is excluded from cells with intact plasma membranes.

Thermo Scientific™ Pierce™ Biotin Quantitation Kit

HABA dye reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in antibodies and other proteins.

Thermo Scientific™ Pierce™ BS3 (Sulfo-DSS) Crosslinker

Crosslink amines to amines with BS3; composed of sulfo-NHS-ester groups separated by an 11.4A, water-soluble spacer arm that is useful for cell-surface protein crosslinking.

Thermo Scientific™ Pierce™ In-Solution Tryptic Digestion and Guanidination Kit

Alkylate and digest proteins and then guanidinate the peptide fragments for mass spectrometry (MS) using proteomics-grade trypsin, buffers and reagents.

Thermo Scientific™ Pierce™ TCEP·HCl

Reduce protein and peptide disulfide bonds using this thiol-free, pure, crystalline tris (2-carboxyethyl) phosphine hydrochloride, TCEP (CAS 5961-85-3).

Thermo Scientific™ Pierce™ DSP Crosslinker

Crosslink amines to amines with dithiobis succinimidyl propionate, composed of NHS-ester groups and disulfide-containing, 12.0A spacer arm and cleavable with reducing agents.

Thermo Scientific™ Pierce™ EDC Crosslinker

Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.

Thermo Scientific™ Pierce™ Human Rinovirus 3C Protease (HRV 3C)

Remove fusion tags from proteins with the HRV 3C cleavage sequence with this recombinant cysteine protease that is dual tagged for easy removal from the sample after cleavage.

Thermo Scientific™ Pierce™ NHS Crosslinker

Prepare amine-reactive NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysuccinimide, CAS 6066-82-6.

Thermo Scientific™ Pierce™ TPCK Trypsin and Resin

Cleave the carboxyl-side of Arg and Lys residues with trypsin that isTPCK-treated to block chymotrypsin activity; its immobilized form allows enzyme removal after digestion.

Thermo Scientific™ Pierce™ HABA Reagent

A colored, biotin assay reagent HABA (4'-hydroxyazobenzene-2-carboxylic acid) to measure biotinylation levels of biotinylated antibodies or proteins.

Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-LC-Biotin

Biotinylate protein amines (e.g., lysines) with this doubly long (30.5A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.

Thermo Scientific™ Pierce™ Sulfo-NHS Crosslinker

Prepare amine-reactive sulfo-NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysulfosuccinimide, CAS 106627-54-7.

Thermo Scientific™ Pierce™ Immobilized Pepsin

Generate and purify Fab and F(ab')2 fragments from antibodies with acidic endopeptidase (pepsin) immobilized onto beaded agarose resin.

Thermo Scientific™ Pierce™ DTT (Dithiothreitol)

Reduce disulfides in biological buffers and sample loading buffers with pure, crystalline dithiothreitol (DTT), the most popular protein disulfide reducing agent.

Thermo Scientific™ Pierce™ In-Gel Tryptic Digestion Kit

Trypsin-digest and recover peptide fragments for mass spectrometry from 150 stained protein bands excised from SDS-PAGE (polyacrylamide gel plugs).

Thermo Scientific™ Pierce™ LC-SMCC Crosslinker

Crosslink amines to sulfhydryls with this long crosslinker composed of NHS-ester and maleimide groups at ends of a stabilizing cyclohexane-containing, 15-atom spacer arm.

Thermo Scientific™ Pierce™ MPBH Crosslinker

Crosslink sulfhydryls to periodate-treated glycoprotein sugars with this long (17.9A) crosslinker containing maleimide and hydrazide groups.

Thermo Scientific Pierce™ Sulfo-SANPAH, No-Weigh™ Format Crosslinker

Thermo Scientific™ Pierce™ Sulfo-SANPAH is hetero-bifunctional crosslinker that contains amine-reactive N-hydroxysuccinimide (NHS) ester and photoactivatable nitrophenyl azide.

Thermo Scientific™ EZ-Link™ Hydrazide-Biotin

Biotinylate glycoproteins (aldehydes of oxidized sugars) with this simplest and shortest (15.7A) hydrazide-activated biotinylation reagent.

Thermo Scientific™ Pierce™ Proteases for Mass Spectrometry

Improve sequence coverage and protein identification for mass spectrometry using these individual, highly specific proteases in custom combinations.

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