Crosslinking, Labeling and Protein Modification

Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotin No-Weigh™ Format

Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotin No-Weigh™ Format is a short-chain, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

Thermo Scientific™ EZ-Link™ NHS-PEG4-Biotin No-Weigh™ Format

Thermo Scientific™ EZ-Link™ NHS-PEG4-Biotin No-Weigh™ Format is a pegylated, water-soluble reagent for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing macromolecules.

Invitrogen™ eBioscience™ 7-AAD Viability Staining Solution

Ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis. Can be used in combination with PE- and FITC-conjugated antibodies in 2-color analysis. The advantage of 7-AAD over PI is that there is minimal spectral overlap between these emissions.

Thermo Scientific™ Pierce™ Immobilized TCEP Disulfide Reducing Gel

Efficiently reduce protein or peptide disulfide bonds with this stable, odorless, beaded agarose resin on which Tris (2-carboxyethyl) phosphine is immobilized.

Thermo Scientific™ Pierce™ Urea

Pure, crystalline urea (CH4N2O), CAS 57-13-6; denaturant with low UV-absorptivity for protein biology methods (elution, solubilization, refolding, storage).

Thermo Scientific™ Pierce™ DMP Crosslinker

Crosslink amines to amines with dimethyl pimelimidate (CAS 58537-94-3); composed of imidoester groups and a membrane-permeable, 7-atom spacer arm.

Thermo Scientific™ Pierce™ 8M Guanidine·HCl Solution

Ready-to-use, 8M solution of guanidinium hydrochloride (GdnCl, GuHCl), CAS 50-01-1; dilute and pH-adjust to any concentration for protein biology methods.

Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin No-Weigh™ Format

Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin No-Weigh™ Format is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

Thermo Scientific™ Pierce™ Biotin Quantitation Kit

HABA dye reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in antibodies and other proteins.

Thermo Scientific™ Pierce™ In-Solution Tryptic Digestion and Guanidination Kit

Alkylate and digest proteins and then guanidinate the peptide fragments for mass spectrometry (MS) using proteomics-grade trypsin, buffers and reagents.

Thermo Scientific™ Pierce™ DSG Crosslinker

Crosslink amines to amines with disuccinimidyl glutarate with NHS-ester groups at the ends of a 7.7A spacer arm; membrane-permeable for intracellular protein crosslinking.

Thermo Scientific™ Pierce™ EDC Crosslinker

Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.

Thermo Scientific™ Bond-Breaker™ Neutral pH TCEP Solution

Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.

Thermo Scientific™ Pierce™ Sulfo-NHS Crosslinker

Prepare amine-reactive sulfo-NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysulfosuccinimide, CAS 106627-54-7.

Thermo Scientific™ Pierce™ Sulfo-SANPAH Crosslinker

Crosslink amines to anything with this long crosslinker composed of Sulfo-NHS-ester and nitrophenyl azide reactive groups; photoactivates with UV light (Amax 320 to 350nm).

Thermo Scientific™ Pierce™ NHS Crosslinker

Prepare amine-reactive NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysuccinimide, CAS 6066-82-6.

Thermo Scientific™ Pierce™ 2-Mercaptoethylamine·HCl

Pure, crystalline 2-aminoethanethiol (2-MEA-HCl, also called cysteamine-HCl), CAS 156-57-0; selectively reduces antibody hinge-region disulfide bonds.

Thermo Scientific™ Pierce™ MPBH Crosslinker

Crosslink sulfhydryls to periodate-treated glycoprotein sugars with this long (17.9A) crosslinker containing maleimide and hydrazide groups.

Thermo Scientific™ Pierce™ FITC and Fluorescein Dyes and Labeling Kits

Activated derivatives of green-fluorescent fluorescein dye to label antibodies, proteins and other molecules for fluorescence imaging (ex 494; em 518).

Thermo Scientific™ Pierce™ DTBP Crosslinker

Crosslink amines to amines with dithiobispropionimidate; composed of imidoester groups at either end of a reducible (cleavable) disulfide-containing spacer arm.

Thermo Scientific™ Pierce™ Sulfo-SMCC Crosslinker

Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker made of NHS-ester and maleimide groups at ends of a cyclohexane-containing, 8-atom spacer arm.

Thermo Scientific™ EZ-Link™ NHS-LC-Biotin

Biotinylate protein amines (e.g., lysines) with this long-chain (22.4A), NHS-ester biotinylation reagent containing a membrane permeable spacer arm for intracellular labeling.

Thermo Scientific™ Pierce™ Guanidine·HCl

Pure, crystalline guanidium hydrochloride (GdnHCl, GuHCl), CAS 50-01-1; denaturant for protein biology methods (elution, solubilization, refolding, storage).

Thermo Scientific™ Pierce™ TCEP·HCl

Reduce protein and peptide disulfide bonds using this thiol-free, pure, crystalline tris (2-carboxyethyl) phosphine hydrochloride, TCEP (CAS 5961-85-3).

Thermo Scientific™ Pierce™ EGS Crosslinker

Crosslink amines to amines with this membrane permeable crosslinker composed of NHS-ester groups and a 16.1A hydroxylamine-cleavable spacer arm.

Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin and Labeling Kits

Biotinylate protein amines (e.g., lysines) with this very popular mid-length (22.4A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.

Thermo Scientific™ EZ-Link™ BMCC-Biotin

Biotinylate reduced cysteines (-SH groups) with a maleimide-activated, sulfhydryl-reactive biotinylation reagent containing a stabilizing cyclohexane group in the spacer arm.

Molecular Probes™ Alexa Fluor™ 594 Antibody Labeling Kit DFS Item

Alexa Fluor™ 594 Antibody Labeling Kit

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