Western Blotting Reagents, Membranes, Supplies

EMD Millipore™ Immobilon™-P PVDF Transfer Membranes

Optimized for binding proteins transferred from a variety of gel matrices. EMD Millipore Immobilon™-P PVDF Transfer Membranes have superior handling, staining, and resistance than nitrocellulose. For use with Western blots and protein sequencing. Available in sheets or rolls.

GE Healthcare Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity.  GE Healthcare Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

GE Healthcare Amersham™ ECL™ Western Blotting Detection Reagents

Use for all routine Western blotting. GE Healthcare Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world’s first commercially available chemiluminescent detection reagents and still one of the most widely used.

GE Healthcare Amersham™ ECL™ Prime Western Blotting Detection Reagent: PROMO

Enable the use of highly diluted primary and secondary antibodies without reducing sensitivity with this detection reagent. GE Healthcare Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

GE Healthcare Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls

Use for the best results with fluorescence Western blotting detection applications. GE Healthcare Life Sciences™ Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

EMD Millipore™ Luminata™ Western HRP Chemiluminescence Substrates

Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns

Thermo Scientific™ SuperSignal™ West Femto Chemiluminescent Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

Thermo Scientific™ Pierce™ Far-Western Blot Kit for Biotinylated Proteins

Detect proteins in gels or on membranes via interaction with biotin-labeled bait proteins as primary probes.

GE Healthcare Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls

Use this versatile nitrocellulose membrane for excellent binding affinity for small proteins and peptides, as well as nucleic acids. GE Healthcare Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

Thermo Scientific™ Pierce™ NBT/BCIP Substrate Solutions

Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.

GE Healthcare Amersham™ ECL™ Western Blotting Detection Reagents: PROMO

Use for all routine Western blotting. GE Healthcare Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world’s first commercially available chemiluminescent detection reagents and still one of the most widely used.

EMD Millipore™ Immobilon™ FL PVDF Transfer Membranes

Designed specifically for Western blots and protein sequencing. EMD Millipore Immobilon™-FL PVDF Transfer Membranes are plain surfaced with 0.45μm pores and have lower background fluorescence than other membranes. Available in 10cm and 20cm squares and a 3.75m roll.

GE Healthcare Amersham™ ECL Select™ Western Blotting Detection Reagent

Use as a high sensitivity reagent for chemiluminescent Western blotting detection. GE Healthcare Life Sciences™ Amersham™ ECL Select™ Western Blotting Detection Reagent  provides highly intense signal output, for detection of medium to very low protein levels.

Thermo Scientific™ PVDF Transfer Membrane, 0.2 µm, 26.5 cm x 3.75 m

Thermo Scientific Pierce PVDF Transfer Membranes are made of high-quality polyvinylidene difluoride and provide high binding capacity for proteins and nucleic acids for Western, Southern, and Northern blotting methods. This PVDF membrane has a 0.2-micron pore size and is ideal for colorimetric and chemiluminescent detection.

Thermo Scientific™ 1-Step™ Ultra TMB-Blotting Solution

Thermo Scientific™ 1-Step Ultra TMB-Blotting Solution is an enhanced single-component horseradish peroxidase (HRP) substrate for Western blotting and immunohistochemistry.

GE Healthcare Amersham™ Hybond™ PVDF Membranes: Rolls: PROMO

Ideal for Western blotting applications where stripping and reprobing are necessary.  GE Healthcare Life Sciences™ Amersham™ Hybond™ PVDF Membranes: Rolls  are hydrophobic PVDF membranes.

GE Healthcare Amersham™ Hybond™-N Membranes

Use for high sensitivity applications in DNA and RNA blotting. GE Healthcare Life Sciences™Amersham™ Hybond™-N Membranes is a strong supported membrane that is inherently hydrophilic and requires no pre-wetting.

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate: PROMO

Buy 3 for the price of 2

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Whatman™ Grade GB003 Blotting Paper: PROMO

Ensures that no contamination will occur during the transfer steps. Whatman™ Grade GB003 Blotting Paper eliminates sheet to sheet variations with precisely cut membrane sizes.

GE Healthcare Amersham™ ECL™ start Western Blotting Detection Reagent: PROMO

Reagent is luminol-based chemiluminescent substrate designed for the detection of proteins immobilized to a membrane

Thermo Scientific™ SuperSignal™ West Dura Chemiluminescent Substrate

Luminol-based enhanced chemiluminescence (ECL) HRP substrate with very stable light output for femtogram-level detection with CCD-based imagers.

GE Healthcare Amersham™ Hybond™ P 0.45μm PVDF Membrane

Use for chemiluminescent and fluorescent detection methods for proteins of > Mr 20 000. GE Healthcare Life Sciences™ Amersham™ Hybond™ P 0.45μm PVDF Membrane is a 0.45 μm pore size hydrophobic membrane, with high physical strength, highly suitable for stripping and reprobing.

Whatman™ Gel Blotting Paper Grade GB003

A thick paper ideal for lysis/denaturation of colony or plaque lifts and Western blots

Whatman™ Grade GB003 Blotting Paper

Ensures that no contamination will occur during the transfer steps. Whatman™ Grade GB003 Blotting Paper eliminates sheet to sheet variations with precisely cut membrane sizes.

EMD Millipore™ Immobilon™ PVDF Transfer Membranes: PSQ

For Western blots and protein sequencing

Thermo Scientific™ SuperSignal™ West Dura Chemiluminescent Substrate: PROMO

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Luminol-based enhanced chemiluminescence (ECL) HRP substrate with very stable light output for femtogram-level detection with CCD-based imagers.

Thermo Scientific™ Pierce™ TMB Substrate Solution

A ready-to-use substrate system containing 3.3', 5.5'-tetramethylbenzidine (TMB)

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