Protein Extraction and Purification
Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagents™
CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: Sodium Lauryl Sulfate, SDS PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]
EMD Millipore™ BioPak™ Disposable Ultrafilter
Nuclease-free, pyrogen-free water from any EMD Millipore Ultrapure™ water system
Invitrogen™ Magnetic Stand-96
Designed for paramagnetic bead precipitation from standard 96-well, U-bottom microplates and 0.2mL PCR plates without additional accessories
Triton™ X-100 (Electrophoresis), Fisher BioReagents™
CAS: 9002-93-1 Molecular Formula: C16H26O2 Molecular Weight (g/mol): 250.382 InChI Key: JYCQQPHGFMYQCF-UHFFFAOYSA-N Synonym: Polyethylene Glycol p-tert-Octylphenyl Ether PubChem CID: 5590 IUPAC Name: 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol SMILES: CC(C)(C)CC(C)(C)C1=CC=C(C=C1)OCCO
SDS Solution, 20% Sodium Dodecyl Sulfate Solution, Molecular Biology/Electrophoresis, Fisher BioReagents™
CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: Sodium lauryl sulfate, SDS PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]
Thermo Scientific™ Pierce™ Sodium Deoxycholate
Water-soluble, ionic detergent used in cell lysis buffers (e.g., RIPA buffer) to disrupt protein interactions for liposome and membrane protein isolation.
Sodium Dodecyl Sulfate (SDS), Micropellets, Fisher BioReagents
CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]
GE Healthcare Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns
For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. GE Healthcare Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.
GE Healthcare Thrombin Protease
For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors
Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktails
Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.
Thermo Scientific™ Pierce Protease Inhibitor Tablets
Thermo Scientific™ Pierce Protease Inhibitor Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments
Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets
Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments
GE Healthcare Streptavidin Sepharose™ High Performance Medium
Used for convenient, fast, reliable purification of biotinylated compounds. Streptavidin Sepharose™ High Performance Medium is a physically and chemically stable medium.
Thermo Scientific™ Pierce™ RIPA Buffer
Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent.
Thermo Scientific™ T-PER™ Tissue Protein Extraction Reagent
Extract total protein from tissue samples using this mild cell lysis and extraction reagent developed specifically for tissues.
EMD Millipore™ Novagen™ Benzonase™ Nuclease, >99%
Degrades all forms of single strand, double stranded, linear, circular DNA RNA.
GE Healthcare Sera-Mag SpeedBeads™ Carboxyl Magnetic Beads, hydrophobic
Excellent for coupling oligos or proteins directly to the beads. Their “cauliflower-like” structure gives Sera-Mag beads high binding capacity. The carboxyl groups on these beads can be activated by standard EDC/EDAC chemistry to react with free amino groups, forming amide bonds.
GE Healthcare Protein G Sepharose™ 4 Fast Flow Media
Rapid processing of large volumes, recoveries of 70 to 90%. GE Healthcare Protein G Sepharose™ 4 Fast Flow Media is recombinant protein G coupled to Sepharose 4 Fast Flow.
Gibco™ Expi293™ Expression System Kit
Designed with transient expression technology for rapid and ultra high-yield protein production from mammalian cells. Gibco™ Expi293™ Expression System Kit is based on high density culture of Expi293F™ cells in Expi293™ Expression Medium.
EMD Millipore™ Calbiochem™ Protease Inhibitor Cocktail Set III, EDTA-Free
Protease inhibitor cocktail III, EDTA-free for inhibiting aspartic, cysteine, and serine proteases as well as aminopeptidases in mammalian cells and tissues.
GE Healthcare HisTrap™ HP Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cells. GE Healthcare HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Thermo Scientific™ Halt™ Protease Inhibitor Cocktails
Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.
Pierce™ Chromogenic Endotoxin Quant Kit
A highly sensitive endpoint assay that accurately measures and detects endotoxin (lipopolysaccharide) in a protein, peptide, nucleic acids or antibody sample using the Amebocyte Lysate Assay.
EMD Millipore™ Novagen™ Streptavidin Agarose
Consists of cross-linked agarose covalently coupled with pure streptavidin.
Thermo Scientific™ Pierce™ Streptavidin Magnetic Beads
Purify or immunoprecipitate biotinylated proteins or affinity ligands using streptavidin-coated magnetic particles for magnet-based separation.
Thermo Scientific™ Pierce™ Mitochondria Isolation Kit for Cultured Cells
Isolate intact mitochondria from cultured mammalian cell samples in about 40 minutes with this versatile, benchtop microcentrifuge-based fractionation kit.
GE Healthcare LabMate™ PD-10 Buffer Reservoirs
Use for high-recovery desalting, buffer exchange and removal of low-molecular weight compounds packed with Sephadex™ G-25 Medium. GE Healthcare Life Sciences™ LabMate™ PD-10 Buffer Reservoirs provide fast, affordable performance.
GE Healthcare His GraviTrap™ Columns
Use for purification of histidine-tagged proteins using gravity flow. His GraviTrap™ Columns enable fast and simple purification.
Thermo Scientific™ Pierce™ Protein Refolding Kit
Refold recombinant proteins that have been denatured and solubilized from inclusion bodies by determining optimal buffer conditions using this reagent matrix strategy.
GE Healthcare Sera-Mag™ Streptavidin Magnetic Beads, high biotin binding capacity
Their “cauliflower-like” structure gives Sera-Mag beads high binding capacity. Sera-Mag Streptavidin beads contain covalently bound streptavidin and are available with low, medium, or high nominal biotin binding capacities. Choose the biotin-binding capacity needed to optimize your application.