Protein Extraction and Purification

Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagents™

Chemical Name or Material: Sodium Dodecyl Sulfate CAS: 151-21-3 CAS: 7647-14-5 CAS: 7757-82-6 Purity Grade: Electrophoresis Grade Purity Grade Notes: DNase- and RNase-Free Physical Form: Solid Assay: ≥99% (≥98% C12-OH) Assay Percent Range: (as Fatty Alcohol Sulfate) >=99% Assay Percent Notes: (as Fatty Alcohol Sulfate) >=99% Molecular Formula: C12H25NaO4S Linear Formula: CH3(CH2)10CH2OSO3Na Formula Weight: 288.38 Synonym: SDS, Sodium Lauryl Sulfate

Triton™ X-100 (Electrophoresis), Fisher BioReagents™

Chemical Name or Material: Triton™ X-100 CAS: 9002-93-1 Purity Grade: Electrophoresis Grade Physical Form: Liquid Linear Formula: 4-(C8H17) C6H4 (OCH2CH2)n OH (n∽10) Formula Weight: 646.86 Synonym: Polyethylene Glycol p-tert-Octylphenyl Ether

EMD Millipore™ BioPak™ Disposable Ultrafilter

Nuclease-free, pyrogen-free water from any EMD Millipore Ultrapure™ water system

Thermo Scientific™ Pierce™ RIPA Buffer

Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent.

Thermo Scientific™ Pierce™ Anti-HA Magnetic Beads

Perform immunoprecipitation of recombinant HA-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators.

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments

SDS Solution, 20% Sodium Dodecyl Sulfate Solution, Molecular Biology/Electrophoresis, Fisher BioReagents™

Chemical Name or Material: Sodium Dodecyl Sulfate Name Note: 20% Solution CAS: 151-21-3 CAS: 7732-18-5 Purity Grade: Electrophoresis/Molecular Biology-Grade Purity Grade Notes: DNase-, RNase- and Protease-Free Physical Form: Liquid Assay: ≥99% Assay Percent Range: (as Fatty Alcohol Sulfate): ≥99% Molecular Formula: C12H25NaO4S Linear Formula: CH3(CH2)10CH2OSO3Na Formula Weight: 288.38 Synonym: SDS, Sodium lauryl sulfate

Thermo Scientific™ Halt™ Phosphatase Inhibitor Cocktail

Stabilize serine, threonine and tyrosine phosphorylation of proteins in cells and tissues with this cocktail of phosphatase inhibitors.

Thermo Scientific™ T-PER™ Tissue Protein Extraction Reagent

Extract total protein from tissue samples using this mild cell lysis and extraction reagent developed specifically for tissues.

Thermo Scientific™ NE-PER™ Nuclear and Cytoplasmic Extraction Kit

Efficiently lyse cells and extract separate cytoplasmic and nuclear protein fractions in less than two hours with this subcellular fractionation kit.

Thermo Scientific™ HisPur™ Ni-NTA Resin

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Thermo Scientific™ Pierce™ Microcentrifuge Spin Cups and Spin Columns

Perform small-scale affinity methods, such as immunoprecipitation assays with agarose beads using these empty spin columns and cups (5 to 500µL).

Thermo Scientific™ Halt™ Protease Inhibitor Cocktails

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.

Thermo Scientific™ B-PER™ Bacterial Protein Extraction Reagents

Efficiently lyse E.coli and other bacterial cells and gently extract native proteins with these nonionic detergent solutions.

Thermo Scientific™ Pierce™ Sodium Deoxycholate

Water-soluble, ionic detergent used in cell lysis buffers (e.g., RIPA buffer) to disrupt protein interactions for liposome and membrane protein isolation.

GE Healthcare Sera-Mag SpeedBeads™ Carboxyl Magnetic Beads, hydrophilic

Excellent for coupling oligos or proteins directly to the beads. Their “cauliflower-like” structure gives Sera-Mag beads high binding capacity. The carboxyl groups on these beads can be activated by standard EDC/EDAC chemistry to react with free amino groups, forming amide bonds.

Invitrogen™ Magnetic Stand-96 DFS Item

Designed for paramagnetic bead precipitation from standard 96-well, U-bottom microplates and 0.2mL PCR plates without additional accessories

EMD Millipore™ Novagen™ Benzonase™ Nuclease, >99%

Degrades all forms of single strand, double stranded, linear, circular DNA RNA.

GE Healthcare Thrombin Protease

For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors

Thermo Scientific™ Pierce™ LAL Chromogenic Endotoxin Quantitation Kit

Accurately measure endotoxin levels (LPS, lipopolysaccharides) in protein, peptide or antibody samples using our Limulus Amebocyte Lysate (LAL) assay.

Sodium Dodecyl Sulfate (SDS), Micropellets, Fisher BioReagents

Chemical Name or Material: Sodium Dodecyl Sulfate (SDS), Micropellets CAS: 151-21-3 Purity Grade: Molecular Biology Grade Physical Form: Solid Assay: ≥98.0% as C12 alcohol Assay Percent Range: >99%

Promega™ Nano-Glo™ Luciferase Assay System DFS Item

Provides a single-addition reagent that generates a glow-type signal in the presence of NanoLuc™ luciferase

GE Healthcare Life Sciences Protein G GraviTrap™

Includes ten ready-to-use columns prepacked with 1ml of Protein G Sepharose 4 Fast Flow

Thermo Scientific™ Pierce™ Mitochondria Isolation Kit for Cultured Cells

Isolate intact mitochondria from cultured mammalian cell samples in about 40 minutes with this versatile, benchtop microcentrifuge-based fractionation kit.

Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktails

Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.

Thermo Scientific™ Tween-80 Detergent Surfact-Ams™ Solution

Thermo Scientific™ Surfact-Amps 80 Detergent Solution is highly-purified Tween-80 detergent stabilized and supplied as a 10% solution in 10mL glass ampules.

Thermo Scientific™ Pierce Phosphatase Inhibitor Mini Tablets

Thermo Scientific™ Pierce Phosphatase Inhibitor Mini Tablets provide complete cell lysate and biological sample protection from dephosphorylation with a broad spectrum phosphatase inhibitor formulation.

GE Healthcare Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. GE Healthcare Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

GE Healthcare Sera-Mag SpeedBeads™ Carboxyl Magnetic Beads, hydrophobic

Excellent for coupling oligos or proteins directly to the beads. Their “cauliflower-like” structure gives Sera-Mag beads high binding capacity. The carboxyl groups on these beads can be activated by standard EDC/EDAC chemistry to react with free amino groups, forming amide bonds.

Thermo Scientific™ Pierce™ Protease Inhibitors

Customize your protease inhibitor cocktail formulation with these small packages of individual protease inhibitor peptides and compounds.

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