Protein Extraction and Purification

EMD Millipore™ BioPak™ Disposable Ultrafilter

Nuclease-free, pyrogen-free water from any EMD Millipore Ultrapure™ water system

Triton™ X-100 (Electrophoresis), Fisher BioReagents™

CAS: 9002-93-1 Molecular Formula: C16H26O2 Molecular Weight (g/mol): 250.382 InChI Key: JYCQQPHGFMYQCF-UHFFFAOYSA-N Synonym: Polyethylene Glycol p-tert-Octylphenyl Ether PubChem CID: 5590 IUPAC Name: 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol SMILES: CC(C)(C)CC(C)(C)C1=CC=C(C=C1)OCCO

Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: Sodium Lauryl Sulfate, SDS PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments

Thermo Scientific™ Pierce™ Sodium Deoxycholate

Water-soluble, ionic detergent used in cell lysis buffers (e.g., RIPA buffer) to disrupt protein interactions for liposome and membrane protein isolation.

Invitrogen™ Magnetic Stand-96 DFS Item

Designed for paramagnetic bead precipitation from standard 96-well, U-bottom microplates and 0.2mL PCR plates without additional accessories

GE Healthcare Thrombin Protease

For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors

Thermo Scientific™ Pierce™ RIPA Buffer

Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent.

SDS Solution, 20% Sodium Dodecyl Sulfate Solution, Molecular Biology/Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: Sodium lauryl sulfate, SDS PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]

Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktails

Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.

Thermo Scientific™ Pierce Protease Inhibitor Tablets

Thermo Scientific™ Pierce Protease Inhibitor Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments

EMD Millipore™ Novagen™ Benzonase™ Nuclease, >99%

Degrades all forms of single strand, double stranded, linear, circular DNA RNA.

GE Healthcare Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. GE Healthcare Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

Gibco™ Expi293™ Expression System Kit DFS Item

Designed with transient expression technology for rapid and ultra high-yield protein production from mammalian cells. Gibco™ Expi293™ Expression System Kit is based on high density culture of Expi293F™ cells in Expi293™ Expression Medium.

Thermo Scientific™ T-PER™ Tissue Protein Extraction Reagent

Extract total protein from tissue samples using this mild cell lysis and extraction reagent developed specifically for tissues.

Thermo Scientific™ Pierce™ Mitochondria Isolation Kit for Cultured Cells

Isolate intact mitochondria from cultured mammalian cell samples in about 40 minutes with this versatile, benchtop microcentrifuge-based fractionation kit.

GE Healthcare Sera-Mag SpeedBeads™ Carboxyl Magnetic Beads, hydrophobic

Excellent for coupling oligos or proteins directly to the beads. Their “cauliflower-like” structure gives Sera-Mag beads high binding capacity. The carboxyl groups on these beads can be activated by standard EDC/EDAC chemistry to react with free amino groups, forming amide bonds.

Sodium Dodecyl Sulfate (SDS), Micropellets, Fisher BioReagents

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]

GE Healthcare Streptavidin Sepharose™ High Performance Medium

Used for convenient, fast, reliable purification of biotinylated compounds. Streptavidin Sepharose™ High Performance Medium is a physically and chemically stable medium.

EMD Millipore™ Prep/Scale Spiral-Wound Ultrafiltration Modules: TFF-2

For easy, reliable preparation of samples ranging from 100L down to 100mL

EMD Millipore™ Upstate Protein G Agarose, Fast Flow

Used for medium low pressure chromatography of IgG from mouse, sheep, rabbit, for immunoprecipitations.

Invitrogen™ eBioscience™ RBC Lysis Buffer, 1x

Formulated for optimal lysis of erythrocytes in single-cell suspensions of mouse hematopoietic tissues such as spleen and human peripheral blood.

EMD Millipore™ Novagen™ Thrombin Cleavage Capture Kit

Designed for cleavage of fusion proteins.

Thermo Scientific™ Pierce™ Tissue Strainers

Rapidly separate cells or cell lysate from tissue debris after mechanical disruption using a 250-micron mesh filter and gravity or gentle centrifugation.

Thermo Scientific™ Pierce™ Protein Refolding Kit

Refold recombinant proteins that have been denatured and solubilized from inclusion bodies by determining optimal buffer conditions using this reagent matrix strategy.

GE Healthcare HisTrap™ FF Columns: 5 x 5mL

Optimize purifications of histidine-tagged proteins directly from homogenized, unclarified cells HisTrap™ FF Columns: 5 x 5mL are prepacked with precharged Ni Sepharose™ 6 Fast Flow media.

Thermo Scientific™ Pierce™ Lysozyme

Increase protein or nucleic acid extraction efficiency with this enzyme that is characterized by the ability to break down the bacterial cell wall.

GE Healthcare SOURCE™ 15Q

Strong anion exchanger with high chemical stability for wide range of working conditions with good resistance to cleaning conditions at high pH

GE Healthcare Superose™ 6 Increase Columns

High-resolution size-exclusion chromatography for studying large proteins and complexes.   GE Healthcare Lifescience™ Superose™ 6 Increase Columns delivers high quality in size exclusion chromatography (SEC).

GE Healthcare Sera-Mag™ Streptavidin Magnetic Beads, high biotin binding capacity

Their “cauliflower-like” structure gives Sera-Mag beads high binding capacity. Sera-Mag Streptavidin beads contain covalently bound streptavidin and are available with low, medium, or high nominal biotin binding capacities. Choose the biotin-binding capacity needed to optimize your application.

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