Protein Biology

Thermo Scientific™ Pierce™ BCA™ Protein Assay

Green-to-blue (A562nm), precise, detergent-compatible assay reagent to measure total protein concentration vs. protein standard.

Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: Sodium Lauryl Sulfate, SDS PubChem CID: 3423265 ChEBI: CHEBI:8984

Thermo Scientific™ Pierce™ Bovine Serum Albumin Standards

High-quality, calibrated BSA solutions (2mg/mL) to serially dilute to create protein assay standard curves to accurately measure protein concentration.

EMD Millipore™ Immobilon™-P PVDF Transfer Membranes

Optimized for binding proteins transferred from a variety of gel matrices. EMD Millipore Immobilon™-P PVDF Transfer Membranes have superior handling, staining, and resistance than nitrocellulose. For use with Western blots and protein sequencing. Available in sheets or rolls.

Invitrogen™ Subcloning Efficiency™ DH5α Competent Cells DFS Item

Economical for routine subcloning procedures or applications where starting DNA is not limiting

Thermo Scientific™ Pierce™ Coomassie (Bradford) Protein Assay

Brown-to-purple (A595nm), ready-to-use, classic reducing agent-compatible assay reagent to measure total protein concentration vs. protein standard.

GE Healthcare Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity.  GE Healthcare Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

Triton™ X-100 (Electrophoresis), Fisher BioReagents™

CAS: 9002-93-1 Molecular Formula: C16H26O2 Molecular Weight (g/mol): 250.382 InChI Key: JYCQQPHGFMYQCF-UHFFFAOYSA-N Synonym: Polyethylene Glycol p-tert-Octylphenyl Ether PubChem CID: 5590

EMD Millipore™ BioPak™ Disposable Ultrafilter

Nuclease-free, pyrogen-free water from any EMD Millipore Ultrapure™ water system

GE Healthcare Disposable PD-10 Desalting Columns

Use for desalting and buffer exchange, as well as removal of low-molecular weight compounds. GE Healthcare Life Sciences™ Disposable PD-10 Desalting Columns provide fast, high-recovery.

Thermo Scientific™ 6X DNA Loading Dye

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.

Invitrogen™ One Shot™ Stbl3™ Chemically Competent E. coli DFS Item

Designed especially for cloning direct repeats found in lentiviral expression vectors

GE Healthcare Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls

Use for the best results with fluorescence Western blotting detection applications. GE Healthcare Life Sciences™ Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

Thermo Scientific™ Pierce™ Methanol-free Formaldehyde Ampules

Vials of high-quality, methanol-free formaldehyde, a general IHC fixative and reversible amine-crosslinking agent for protein and nucleic acid samples.

Thermo Scientific™ Pierce™ Micro BCA™ Protein Assay

Special 3-component version of our popular BCA Reagents to measure total protein concentration of dilute protein solutions (0.5 to 20 micrograms/mL).

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments

Invitrogen™ eBioscience™ 7-AAD Viability Staining Solution

Ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis. Can be used in combination with PE- and FITC-conjugated antibodies in 2-color analysis. The advantage of 7-AAD over PI is that there is minimal spectral overlap between these emissions.

GE Healthcare Amersham™ ECL™ Western Blotting Detection Reagents

Use for all routine Western blotting. GE Healthcare Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world’s first commercially available chemiluminescent detection reagents and still one of the most widely used.

EMD Millipore™ Immobilon™ FL PVDF Transfer Membranes

Designed specifically for Western blots and protein sequencing. EMD Millipore Immobilon™-FL PVDF Transfer Membranes are plain surfaced with 0.45μm pores and have lower background fluorescence than other membranes. Available in 10cm and 20cm squares and a 3.75m roll.

Corning™ Laminin, Mouse

Promotes cell adhesion, migration, growth, and differentiation, including neurite outgrowth

Thermo Scientific™ Slide-A-Lyzer™ 10K MWCO G2 Dialysis Cassettes

Remove buffer salts and contaminants while maximizing sample recovery with these second-generation self-floating protein dialysis cassettes (10K MWCO).

Invitrogen™ Novex™ NuPAGE™ MES SDS Running Buffer (20X) DFS Item

Made with high-purity reagents and are strictly quality controlled

EMD Millipore™ Poly-L-Lysine Solution

Used as coating to enhance cell attachment and adhesion to both plasticware and glass surfaces

Thermo Scientific™ Pierce™ RIPA Buffer

Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent.

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