Protein Biology

EMD Millipore™ Immobilon™-P PVDF Transfer Membranes

Optimized for binding proteins transferred from a variety of gel matrices. EMD Millipore Immobilon™-P PVDF Transfer Membranes have superior handling, staining, and resistance than nitrocellulose. For use with Western blots and protein sequencing. Available in sheets or rolls.

Thermo Scientific™ Pierce™ BCA™ Protein Assay

Green-to-blue (A562nm), precise, detergent-compatible assay reagent to measure total protein concentration vs. protein standard.

Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagents™

Chemical Name or Material: Sodium Dodecyl Sulfate CAS: 151-21-3 CAS: 7647-14-5 CAS: 7757-82-6 Purity Grade: Electrophoresis Grade Purity Grade Notes: DNase- and RNase-Free Physical Form: Solid Assay: ≥99% (≥98% C12-OH) Assay Percent Range: (as Fatty Alcohol Sulfate) >=99% Assay Percent Notes: (as Fatty Alcohol Sulfate) >=99% Molecular Formula: C12H25NaO4S Linear Formula: CH3(CH2)10CH2OSO3Na Formula Weight: 288.38 Synonym: SDS, Sodium Lauryl Sulfate

Invitrogen™ Subcloning Efficiency™ DH5α Competent Cells DFS Item

Economical for routine subcloning procedures or applications where starting DNA is not limiting

GE Healthcare Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity.  GE Healthcare Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

Thermo Scientific™ Pierce™ Bovine Serum Albumin Standards

High-quality, calibrated BSA solutions (2mg/mL) to serially dilute to create protein assay standard curves to accurately measure protein concentration.

Triton™ X-100 (Electrophoresis), Fisher BioReagents™

Chemical Name or Material: Triton™ X-100 CAS: 9002-93-1 Purity Grade: Electrophoresis Grade Physical Form: Liquid Linear Formula: 4-(C8H17) C6H4 (OCH2CH2)n OH (n∽10) Formula Weight: 646.86 Synonym: Polyethylene Glycol p-tert-Octylphenyl Ether

GE Healthcare Disposable PD-10 Desalting Columns

Use for desalting and buffer exchange, as well as removal of low-molecular weight compounds. GE Healthcare Life Sciences™ Disposable PD-10 Desalting Columns provide fast, high-recovery.

GE Healthcare Amersham™ ECL™ Prime Western Blotting Detection Reagent: PROMO

Enable the use of highly diluted primary and secondary antibodies without reducing sensitivity with this detection reagent. GE Healthcare Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

GE Healthcare Amersham™ ECL™ Western Blotting Detection Reagents

Use for all routine Western blotting. GE Healthcare Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world’s first commercially available chemiluminescent detection reagents and still one of the most widely used.

Thermo Scientific™ 6X DNA Loading Dye

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

EMD Millipore BioPak™ Disposable Ultrafilter

Nuclease-free, pyrogen-free water from any EMD Millipore Ultrapure™ water system

Thermo Scientific™ Pierce™ Coomassie (Bradford) Protein Assay

Brown-to-purple (A595nm), ready-to-use, classic reducing agent-compatible assay reagent to measure total protein concentration vs. protein standard.

Thermo Scientific™ Pierce™ Methanol-free Formaldehyde Ampules

Vials of high-quality, methanol-free formaldehyde, a general IHC fixative and reversible amine-crosslinking agent for protein and nucleic acid samples.

GE Healthcare Thrombin Protease

For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors

Thermo Scientific™ Pierce™ Micro BCA™ Protein Assay

Special 3-component version of our popular BCA Reagents to measure total protein concentration of dilute protein solutions (0.5 to 20 micrograms/mL).

Thermo Scientific™ SnakeSkin™ Dialysis Tubing Clips

Securely close dialysis tubing up to 50mm flat diameter, including SnakeSkin Dialysis Tubing, with these sturdy, non-floating tube closures.

GE Healthcare Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls

Use for the best results with fluorescence Western blotting detection applications. GE Healthcare Life Sciences™ Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

GE Healthcare Disposable PD-10 Desalting Columns: PROMO

Use for desalting and buffer exchange, as well as removal of low-molecular weight compounds. GE Healthcare Life Sciences™ Disposable PD-10 Desalting Columns provide fast, high-recovery.

Thermo Scientific™ Pierce™ ESI Negative Ion Calibration Solution

Calibrate LTQ mass spectrometer instruments using a mixture of highly purified ionizable molecules designed for negative mode calibration.

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments

EMD Millipore™ Luminata™ Western HRP Chemiluminescence Substrates

Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns

Thermo Scientific™ Pierce™ Silver Stain Kit

Stain proteins in 1D and 2D gels with this rapid, ultra-sensitive and versatile silver stain system that yields consistent and reliable results.

Thermo Scientific™ SuperSignal™ West Femto Chemiluminescent Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

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