Antibiotics, Buffers and Supplements

Gibco™ Trypan Blue Solution, 0.4% DFS Item

Routinely used as a cell stain to assess cell viability using the dye exclusion test

Gibco™ Trypsin-EDTA (0.25%), Phenol red DFS Item

Made from trypsin powder, irradiated mixture of proteases derived from porcine pancreas

Gibco™ L-Glutamine (200mM) DFS Item

Ready-to-use stock solution that acts as a cell culture supplement

Gibco™ GlutaMAX™ Supplement DFS Item

An alternative to L-glutamine, with increased stability that improves cell health

Gibco™ MEM Non-Essential Amino Acids Solution (100X) DFS Item

Used as a growth supplement for cell culture medium, to increase cell growth and viability

Gibco™ 2-Mercaptoethanol (55mM) DFS Item

A potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals

Corning™ Matrigel™ GFR Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ GFR Basement Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.

Gibco™ ACK Lysing Buffer DFS Item

Used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow

BD Bacto™ Dehydrated Culture Media Additive: Tryptone

Enzymatic digest of casein.  BD Difco™ Bacto™ Dehydrated Culture Media Additive: Tryptone is part of a large selection of dehydrated media.

Corning™ Matrigel™ Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.

Gibco™ Trypsin-EDTA (0.05%), Phenol red DFS Item

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

BD Bacto™ Dehydrated Culture Media Additive: Yeast Extract

Formulation of culture media for cultivation of heterotrophic microorganisms.  BD Difco™ Bacto™ Dehydrated Culture Media Additive: Yeast Extract is supplied in dehydrated form for the convenience of the laboratorian and bioprocessing specialist.

Gibco™ HEPES (1M) DFS Item

A zwitterionic organic chemical buffering agent commonly used in cell culture media

Corning™ Matrigel™ hESC-Qualified Matrix

Compatible with mTeSR™1-devloped by StemCell Technologies. Corning™ Matrigel™ hESC-Qualified Matrix is widely accepted as an alternative substrate for the culture of hES cells as well as human induced pluripotent stem (iPS) cells.

MES (Fine White Crystals), Fisher BioReagents

CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.248 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N Synonym: 2-(4-Morpholino)ethane Sulfonic Acid, 2-(N-Morpholino)ethanesulfonic acid hydrate PubChem CID: 16218417 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid;hydrate SMILES: C1COCCN1CCS(=O)(=O)O.O

Gibco™ TrypLE Express Enzyme (1X), No Phenol Red DFS Item

Unlike porcine trypsin, free of animal-derived components

Gibco™ TrypLE Express Enzyme (1X), Phenol Red DFS Item

Animal origin-free, recombinant enzyme

Gibco™ Trypsin-EDTA (0.5%), No Phenol Red DFS Item

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

Corning™ CellStripper Dissociation Reagent

Non-enzymatic cell dissociation solution gently dislodges adherent cells in culture

Corning™ Cell-Tak Cell and Tissue Adhesive

Attach cells or tissue sections to many types of surfaces, including plastic, glass, metal, FEP Polymer, and biological materials. Corning™ Cell-Tak Cell and Tissue Adhesive is a specially formulated protein solution extracted from marine mussel, Mytilus edulis.

Gibco™ Trypsin (2.5%), No Phenol Red DFS Item

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

Gibco™ Pluronic F-68 Non-ionic Surfactant (100X) DFS Item

A non-ionic surfactant used to control shear forces in suspension cultures

Gibco™ Insulin-Transferrin-Selenium (ITS -G) (100X) DFS Item

A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells

Corning™ Cell Recovery Solution

Allows for the recovery of cells cultured on BD Matrigel™ Basement Membrane Matrix for subsequent biochemical analyses

Corning™ DMSO (Dimethyl Sulfoxide)

For cryopreservation of cells along with glycerol, to prevent damage to cell membrane during freezing

Gibco™ Phytohemagglutinin, M form (PHA-M) DFS Item

This extract is capable of inducing blastogenesis in vitro in various mammalian mononuclear cells when used at a concentration of 1 to 2% v/v

HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCO)CCS(=O)(=O)O

Gibco™ HT Supplement (100X) DFS Item

HT-supplemented medium is suitable for post-selection rescue to overcome the effects of residual intracellular aminopterin.

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