Antibiotics, Buffers and Supplements

Gibco™ Trypsin-EDTA (0.25%), Phenol red DFS Item

Made from trypsin powder, irradiated mixture of proteases derived from porcine pancreas

Gibco™ Trypan Blue Solution, 0.4% DFS Item

Routinely used as a cell stain to assess cell viability using the dye exclusion test

Corning™ Matrigel™ hESC-Qualified Matrix

Compatible with mTeSR™1-devloped by StemCell Technologies. Corning™ Matrigel™ hESC-Qualified Matrix is widely accepted as an alternative substrate for the culture of hES cells as well as human induced pluripotent stem (iPS) cells.

Gibco™ MEM Non-Essential Amino Acids Solution (100X) DFS Item

Used as a growth supplement for cell culture medium, to increase cell growth and viability

Gibco™ GlutaMAX™ Supplement DFS Item

An alternative to L-glutamine, with increased stability that improves cell health

BD Bacto™ Dehydrated Culture Media Additive: Yeast Extract

Formulation of culture media for cultivation of heterotrophic microorganisms.  BD Difco™ Bacto™ Dehydrated Culture Media Additive: Yeast Extract is supplied in dehydrated form for the convenience of the laboratorian and bioprocessing specialist.

Gibco™ L-Glutamine (200mM) DFS Item

Ready-to-use stock solution that acts as a cell culture supplement

Corning™ Matrigel™ Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.

BD Bacto™ Dehydrated Culture Media Additive: Tryptone

Enzymatic digest of casein.  BD Difco™ Bacto™ Dehydrated Culture Media Additive: Tryptone is part of a large selection of dehydrated media.

Corning™ Matrigel™ GFR Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ GFR Basement Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.

Gibco™ HEPES (1M) DFS Item

A zwitterionic organic chemical buffering agent commonly used in cell culture media

Gibco™ TrypLE Express Enzyme (1X), No Phenol Red DFS Item

Unlike porcine trypsin, free of animal-derived components

Gibco™ Insulin-Transferrin-Selenium (ITS -G) (100X) DFS Item

A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells

MES (Fine White Crystals), Fisher BioReagents

CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.248 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N Synonym: 2-(4-Morpholino)ethane Sulfonic Acid, 2-(N-Morpholino)ethanesulfonic acid hydrate PubChem CID: 16218417

Corning™ CellStripper Dissociation Reagent

Non-enzymatic cell dissociation solution gently dislodges adherent cells in culture

Gibco™ Trypsin-EDTA (0.05%), Phenol red DFS Item

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

Gibco™ Trypsin-EDTA (0.5%), No Phenol Red DFS Item

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid PubChem CID: 23831 ChEBI: CHEBI:42334

Corning™ DMSO (Dimethyl Sulfoxide)

For cryopreservation of cells along with glycerol, to prevent damage to cell membrane during freezing

Gibco™ 2-Mercaptoethanol (55mM) DFS Item

A potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals

Gibco™ ACK Lysing Buffer DFS Item

Used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow

Gibco™ Pluronic F-68 Non-ionic Surfactant (100X) DFS Item

A non-ionic surfactant used to control shear forces in suspension cultures

Corning™ 0.25% Trypsin, 0.1% EDTA in HBSS w/o Calcium, Magnesium and Sodium Bicarbonate

Used to enzymatically release adherent cells from tissue culture plates for passaging.

Gibco™ HT Supplement (100X) DFS Item

HT-supplemented medium is suitable for post-selection rescue to overcome the effects of residual intracellular aminopterin.

Corning™ Cell-Tak Cell and Tissue Adhesive

Attach cells or tissue sections to many types of surfaces, including plastic, glass, metal, FEP Polymer, and biological materials. Corning™ Cell-Tak Cell and Tissue Adhesive is a specially formulated protein solution extracted from marine mussel, Mytilus edulis.

Corning™ Matrigel™ Membrane Matrix HC

High concentration (HC) formulation is ideal for applications in which a high protein concentration augments the growth of tumors; allows a Matrigel basement membrane matrix plug to maintain its integrity after subcutaneous injection into mice

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