Antibiotics, Buffers and Supplements

Gibco™ Trypan Blue Solution, 0.4% DFS Item

Routinely used as a cell stain to assess cell viability using the dye exclusion test

Gibco™ Trypsin-EDTA (0.25%), Phenol red DFS Item

Made from trypsin powder, irradiated mixture of proteases derived from porcine pancreas

Corning™ Matrigel™ GFR Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ GFR Basement Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.

Gibco™ GlutaMAX™ Supplement DFS Item

An alternative to L-glutamine, with increased stability that improves cell health

Gibco™ L-Glutamine (200mM) DFS Item

Ready-to-use stock solution that acts as a cell culture supplement

Gibco™ MEM Non-Essential Amino Acids Solution (100X) DFS Item

Used as a growth supplement for cell culture medium, to increase cell growth and viability

Gibco™ Trypsin-EDTA (0.5%), No Phenol Red DFS Item

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

Gibco™ 2-Mercaptoethanol (55mM) DFS Item

A potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals

Gibco™ TrypLE Express Enzyme (1X), No Phenol Red DFS Item

Unlike porcine trypsin, free of animal-derived components

Corning™ Matrigel™ hESC-Qualified Matrix

Compatible with mTeSR™1-devloped by StemCell Technologies. Corning™ Matrigel™ hESC-Qualified Matrix is widely accepted as an alternative substrate for the culture of hES cells as well as human induced pluripotent stem (iPS) cells.

Corning™ CellStripper Dissociation Reagent

Non-enzymatic cell dissociation solution gently dislodges adherent cells in culture

BD Bacto™ Dehydrated Culture Media Additive: Yeast Extract

Formulation of culture media for cultivation of heterotrophic microorganisms.  BD Difco™ Bacto™ Dehydrated Culture Media Additive: Yeast Extract is supplied in dehydrated form for the convenience of the laboratorian and bioprocessing specialist.

Gibco™ ACK Lysing Buffer DFS Item

Used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow

HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCO)CCS(=O)(=O)O

Corning™ Matrigel™ Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.

MES (Fine White Crystals), Fisher BioReagents

CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.248 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N Synonym: 2-(4-Morpholino)ethane Sulfonic Acid, 2-(N-Morpholino)ethanesulfonic acid hydrate PubChem CID: 16218417 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid;hydrate SMILES: C1COCCN1CCS(=O)(=O)O.O

BD Bacto™ Dehydrated Culture Media Additive: Tryptone

Enzymatic digest of casein.  BD Difco™ Bacto™ Dehydrated Culture Media Additive: Tryptone is part of a large selection of dehydrated media.

Gibco™ TrypLE Express Enzyme (1X), Phenol Red DFS Item

Animal origin-free, recombinant enzyme

Corning™ 0.25% Trypsin, 0.1% EDTA in HBSS w/o Calcium, Magnesium and Sodium Bicarbonate

Used to enzymatically release adherent cells from tissue culture plates for passaging.

Corning™ Cell Recovery Solution

Allows for the recovery of cells cultured on BD Matrigel™ Basement Membrane Matrix for subsequent biochemical analyses

Gibco™ Trypsin-EDTA (0.05%), Phenol red DFS Item

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

Gibco™ Insulin-Transferrin-Selenium (ITS -G) (100X) DFS Item

A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells

Corning™ ITS Premix Universal Culture Supplement

Stimulates proliferation of a variety of cells under serum-reduced conditions. Corning™ ITS Premix Universal Culture Supplement contains insulin, human transferrin and selenous acid, the three most universally esential components of defined culture media.

Tricine (White Crystals), Fisher BioReagents

CAS: 5704-04-1 Molecular Formula: C6H13NO5 Molecular Weight (g/mol): 179.17 InChI Key: SEQKRHFRPICQDD-UHFFFAOYSA-N Synonym: N-[Tris-(hydroxymethyl)methyl]glycine PubChem CID: 79784 ChEBI: CHEBI:39063 IUPAC Name: 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid SMILES: C(C(=O)O)NC(CO)(CO)CO

Gibco™ HEPES (1M) DFS Item

A zwitterionic organic chemical buffering agent commonly used in cell culture media

Gibco™ Trypsin (2.5%), No Phenol Red DFS Item

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

  spinner