Enzymes and Inhibitors

EMD Millipore™ Calbiochem™ Lysozyme, Chicken Egg White

Inhibits biological activity of lipopolysaccharides from periodontopathic bacteria

EMD Millipore™ Calbiochem™ Lysozyme, Human Neutrophil

Takes part in antibacterial activity of leukocytes

EMD Millipore™ Calbiochem™ EDTA, Tetrasodium Tetrahydrate Salt

Used in ion exchange, metal chelation, antioxidation procedures, spectrophotometric titration, and other chemical procedures

Hexokinase, yeast, Alfa Aesar™

Used to catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing, ATP as a phosphate source. Used to determine glucose, fructose, mannose and ATP.

EMD Millipore™ Calbiochem™ BQ-788, Sodium Salt

Highly selective ETB-receptor antagonist

Proteinase K, Tritirachium album limber, Alfa Aesar™

Commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of Proteinase K to nucleic acid preparations rapidly inactivates nucleases that might otherwise degrade the DNA or RNA during purification.

Ascorbate oxidase, from Cucurbita sp., Alfa Aesar™

May be used to study oxidative stress and heat stress response and tolerance.

EMD Millipore™ Novagen™ KOD Xtreme™ Hot Start DNA Polymerase

For challenging PCR applications including: crude sample PCR, amplification of long strand and highly GC-rich DNA templates

Peroxidase, horseradish, Alfa Aesar™

Used in the oxidation of luminol to 3-aminophthalate. It is often used in conjugates to determine the presence of a molecular target, especially in the detection of specific protein in a western blot after conjugation with antibody.

Ribonuclease A, bovine pancreas, Molecular Biology Grade, Alfa Aesar™

Used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA.

Pectinase, Aspergillus niger, Alfa Aesar™

Used in plant protoplast preparation to digest cell wall prior to organelle isolation. Pectinase helps facilitate the maceration of plant materials and extraction processes including organelle preparation.

EMD Millipore™ Calbiochem™ Benzamidine, Hydrochloride

Inhibitor of trypsin and trypsin-like enzymes

EMD Millipore™ Calbiochem™ Horseradish Peroxidase

Suitable for conjugation to proteins and antibodies and as cytochemical marker in immunohistochemical staining

Deoxyribonuclease I, bovine pancreas, Purified, Alfa Aesar™

Used in RNA preparations from tissue or bacterial cell cultures. Deoxyribonuclease is used to remove DNA from protein samples, It is also used in a study to compare several procedures for reducing RNase contamination in preparations of DNase.

Collagenase, Type I, Clostridium histolyticum, Alfa Aesar™

Used as a powder and solvent injection kit for the treatment of dupuytren's contracture.

EMD Millipore™ Calbiochem™ MACERASE™ Pectinase, Rhizopus sp.

Useful in isolating intact cells and protoplasts from higher plants

EMD Millipore™ Novagen™ KOD Hot Start DNA Polymerase

For PCR amplification of long strand and GC-rich DNA templates, cloning and cDNA amplification applications

EMD Millipore™ Calbiochem™ Caspase-8 Detection Kit (FITC-IETD-FMK)

Convenient, and sensitive method to measure caspase-8 in living cells

Casein hydrolysate enzymatic, Alfa Aesar™

Used as nutritional source in microbial media. It is derived from the enzymatic digest of casein, often used in culture media. NZ Amine EKC contains a mixture of peptides and amino acids.