Enzymes and Inhibitors

EMD Millipore™ Calbiochem™ N-Acetyl-α-D-Glucosamine

Acceptor substrate for galactosyltransferases

EMD Millipore™ Calbiochem™ Caspase-3 Detection Kit (FITC-DEVD-FMK)

For detection of activated caspase-3 in living cells

EMD Millipore™ Calbiochem™ MACERASE™ Pectinase, Rhizopus sp.

Useful in isolating intact cells and protoplasts from higher plants

Casein hydrolysate enzymatic, Alfa Aesar™

Used as nutritional source in microbial media. It is derived from the enzymatic digest of casein, often used in culture media. NZ Amine EKC contains a mixture of peptides and amino acids.

EMD Millipore™ Calbiochem™ Caspase Detection Kit (Red-VAD-FMK)

For detection of activated caspases in living cells

EMD Millipore™ Calbiochem™ Melittin

Inhibits adenylate cyclase activity in synaptic membranes

Hexokinase, yeast, Alfa Aesar™

Used to catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing, ATP as a phosphate source. Used to determine glucose, fructose, mannose and ATP.

EMD Millipore™ Calbiochem™ Caspase-3 Detection Kit (Red-DEVD-FMK)

For detection of activated caspase-3 in living cells

Deoxyribonuclease I, bovine pancreas, Alfa Aesar™

Used for the removal of DNA from protein samples.

Ribonuclease A, bovine pancreas, Molecular Biology Grade, Alfa Aesar™

Used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA.

Peroxidase, horseradish, Alfa Aesar™

Used in the oxidation of luminol to 3-aminophthalate. It is often used in conjugates to determine the presence of a molecular target, especially in the detection of specific protein in a western blot after conjugation with antibody.

EMD Millipore™ Novagen™ KOD Xtreme™ Hot Start DNA Polymerase

For challenging PCR applications including: crude sample PCR, amplification of long strand and highly GC-rich DNA templates

EMD Millipore™ Calbiochem™ Horseradish Peroxidase

Suitable for conjugation to proteins and antibodies and as cytochemical marker in immunohistochemical staining

Proteinase K, Tritirachium album limber, Alfa Aesar™

Commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of Proteinase K to nucleic acid preparations rapidly inactivates nucleases that might otherwise degrade the DNA or RNA during purification.

EMD Millipore™ Calbiochem™ Lysozyme, Chicken Egg White

Inhibits biological activity of lipopolysaccharides from periodontopathic bacteria

EMD Millipore™ Calbiochem™ Lysozyme, Human Neutrophil

Takes part in antibacterial activity of leukocytes

Ribonuclease A, bovine pancreas, purified, Alfa Aesar™

Used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA.

EMD Millipore™ Novagen™ Chicken Egg White Lysozyme Solution

For purification of both DNA, protein, and Gram-positive bacterial lysis

Pectinase, Aspergillus niger, Alfa Aesar™

Used in plant protoplast preparation to digest cell wall prior to organelle isolation. Pectinase helps facilitate the maceration of plant materials and extraction processes including organelle preparation.

EMD Millipore™ Novagen™ KOD Hot Start DNA Polymerase

For PCR amplification of long strand and GC-rich DNA templates, cloning and cDNA amplification applications

Deoxyribonuclease I, bovine pancreas, Purified, Alfa Aesar™

Used in RNA preparations from tissue or bacterial cell cultures. Deoxyribonuclease is used to remove DNA from protein samples, It is also used in a study to compare several procedures for reducing RNase contamination in preparations of DNase.

  spinner