Enzymes and Inhibitors

Hexokinase, yeast, Alfa Aesar™

Used to catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing, ATP as a phosphate source. Used to determine glucose, fructose, mannose and ATP.

Ribonuclease A, bovine pancreas, Molecular Biology Grade, Alfa Aesar™

Used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA.

EMD Millipore™ Calbiochem™ Horseradish Peroxidase

Suitable for conjugation to proteins and antibodies and as cytochemical marker in immunohistochemical staining

Ribonuclease B, bovine pancreas, Alfa Aesar™

Used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA.

EMD Millipore™ Calbiochem™ MACERASE™ Pectinase, Rhizopus sp.

Useful in isolating intact cells and protoplasts from higher plants

EMD Millipore™ Calbiochem™ Lysozyme, Human Neutrophil

Takes part in antibacterial activity of leukocytes

Pectinase, Aspergillus niger, Alfa Aesar™

Used in plant protoplast preparation to digest cell wall prior to organelle isolation. Pectinase helps facilitate the maceration of plant materials and extraction processes including organelle preparation.

Peroxidase, horseradish, Alfa Aesar™

Used in the oxidation of luminol to 3-aminophthalate. It is often used in conjugates to determine the presence of a molecular target, especially in the detection of specific protein in a western blot after conjugation with antibody.

EMD Millipore™ Calbiochem™ Synthetic N-Acetylneuraminic Acid

Component of mucoproteins, mucopolysaccharides, and mucolipids

EMD Millipore™ Calbiochem™ Lectin, PHA-L, Phaseolus vulgaris

Useful as specific marker in anterograde neuronal tracing

EMD Millipore™ Calbiochem™ Caspase-3 Detection Kit (FITC-DEVD-FMK)

For detection of activated caspase-3 in living cells

Deoxyribonuclease I, bovine pancreas, Purified, Alfa Aesar™

Used in RNA preparations from tissue or bacterial cell cultures. Deoxyribonuclease is used to remove DNA from protein samples, It is also used in a study to compare several procedures for reducing RNase contamination in preparations of DNase.

Proteinase K, Tritirachium album limber, Alfa Aesar™

Commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of Proteinase K to nucleic acid preparations rapidly inactivates nucleases that might otherwise degrade the DNA or RNA during purification.

Casein hydrolysate enzymatic, Alfa Aesar™

Used as nutritional source in microbial media. It is derived from the enzymatic digest of casein, often used in culture media. NZ Amine EKC contains a mixture of peptides and amino acids.

EMD Millipore™ Calbiochem™ N-Acetylneuraminic Acid, 2,3-Dehydro-2-deoxy-, Sodium Salt

Inhibitor of bacterial, viral, and mammalian neuraminidases

EMD Millipore™ Calbiochem™ Caspase Detection Kit (Red-VAD-FMK)

For detection of activated caspases in living cells

Ascorbate oxidase, from Cucurbita sp., Alfa Aesar™

May be used to study oxidative stress and heat stress response and tolerance.

EMD Millipore™ Calbiochem™ Melittin

Inhibits adenylate cyclase activity in synaptic membranes

EMD Millipore™ Novagen™ KOD XL DNA Polymerase

For PCR amplification of long strand and GC-rich DNA templates

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