Biological Buffers

Thermo Scientific™ 6X DNA Loading Dye

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.

Invitrogen™ GlycoBlue™ Coprecipitant (15mg/mL) DFS Item

Blue dye increases pellet visibility

HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCO)CCS(=O)(=O)O

MES (Fine White Crystals), Fisher BioReagents

CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.248 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N Synonym: 2-(4-Morpholino)ethane Sulfonic Acid, 2-(N-Morpholino)ethanesulfonic acid hydrate PubChem CID: 16218417 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid;hydrate SMILES: C1COCCN1CCS(=O)(=O)O.O

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.

Thermo Scientific™ SuperBlock™ Blocking Buffers

Block membranes, plates and tissue samples with these optimized solutions containing a protein that provides fast and effective blocking for Western blot, ELISA and IHC.

Promega™ Blue/Orange Loading Dye, 6X DFS Item

For loading DNA samples onto nondenaturing gels

Invitrogen™ EDTA (0.5M), pH 8.0 DFS Item

Ready-to-use, certified RNase-free

HEPES Buffer, 1M Solution, Cell Culture Grade, MP Biomedicals

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCO)CCS(=O)(=O)O

Tricine (White Crystals), Fisher BioReagents

CAS: 5704-04-1 Molecular Formula: C6H13NO5 Molecular Weight (g/mol): 179.17 InChI Key: SEQKRHFRPICQDD-UHFFFAOYSA-N Synonym: N-[Tris-(hydroxymethyl)methyl]glycine PubChem CID: 79784 ChEBI: CHEBI:39063 IUPAC Name: 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid SMILES: C(C(=O)O)NC(CO)(CO)CO

Invitrogen™ LiCl Precipitation Solution (7.5M) DFS Item

Will not precipitate DNA, protein, or carbohydrates

Thermo Scientific™ Blocker™ Casein Blocking Buffers

Block membranes, plates and other samples with these ready-to-use PBS or TBS solutions of purified casein for Western blot, ELISA and other detection methods.

Invitrogen™ Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1) DFS Item

Premixed, safer, and easier alternative to preparing crystalline phenol solutions

Laemmli SDS Sample Buffer, reducing, 6X

For protein sample preparation to be used in the Laemmli SDS-PAGE system

Invitrogen™ Novex™ NuPAGE™ Tris-Acetate SDS Running Buffer (20X) DFS Item

Formulated for separation of proteins, in their denatured state, on NuPAGE Novex Tris-Acetate gels

Invitrogen™ Novex™ NuPAGE™ MES SDS Running Buffer (20X) DFS Item

Made with high-purity reagents and are strictly quality controlled

GE Healthcare Ab Buffer Kit

Premade stock buffers optimized for purification of monoclonal and polyclonal IgG using immobilized Protein A or Protein G

Invitrogen™ Proteinase K Solution (20mg/mL) DFS Item

General digestion of proteins

Thermo Scientific™ Pierce™ Binding and Elution Buffers for IgG Purification

Ensure high-yield, nondenaturing antibody purification using IgG affinity purification supports with these gentle buffer solutions.

Invitrogen™ UltraPure BSA (50mg/mL) DFS Item

Excellent PCR stabilizer

Invitrogen™ Novex™ NuPAGE™ Transfer Buffer (20X) DFS Item

Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting

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